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Frozen Syringes II


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#1 MrMycelium

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Posted 31 October 2007 - 02:17 PM

Ok, for all of you following this here it is.

My foaf redid the syringes he put in the freezer last night with better ones today.

1 B+, 1 Pan-Cambo, 1 Azure-Hammond

All of the syringes have 8ml of spore solotion not 10ml because of how much
liquid expands when frozen.

How long should he keep them in the freezer? 1 week, 2 weeks, 1 month.

Attached Thumbnails

  • frozen-002.jpg
  • frozen-001.jpg


#2 prism

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Posted 31 October 2007 - 02:19 PM

1 Month should do the trick! Good Luck!:headbang:

#3 lardnar

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Posted 31 October 2007 - 02:22 PM

1 week then see if they're still viable!

If they aren't after a week they aren't after a month

#4 camMyco

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Posted 31 October 2007 - 02:38 PM

I would think after they are frozen solid all damage if any will have occurred. Try it for a week and see what grows. don't forget to leave room in the syringe for h2o expansion.

#5 cheshire

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Posted 31 October 2007 - 02:47 PM

yeah i froze mine by mistake and they didnt work... :(

#6 MrMycelium

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Posted 31 October 2007 - 02:49 PM

yeah i froze mine by mistake and they didnt work... :(


This is why my foaf used different strains. Woodlovers, Poo lovers, for lovers. lol

#7 darknezz7

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Posted 31 October 2007 - 05:39 PM

I've had syringes ice over a little for a few days, they were not completely solid. They ended up being fine, they were poo lovers.

This will be good to know and yeah I'd say a week is plenty.

#8 koldj

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Posted 31 October 2007 - 05:46 PM

I would think after they are frozen solid all damage if any will have occurred. Try it for a week and see what grows. don't forget to leave room in the syringe for h2o expansion.

I agree. One day or one week at 0 wouldn't change a lot. So IMO you may even try them tomorrow.

#9 dta08

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Posted 31 October 2007 - 11:32 PM

I say a week

#10 gsmith1981

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Posted 02 November 2007 - 07:35 AM

cant wait to see the results hows thoughs pan cambos?
:hookah:

#11 MrMycelium

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Posted 02 November 2007 - 11:21 AM

My foaf said he would take them out on the 7th.

Like everyone says, a week should be good.

Will post pics today sometime of how things are going.

#12 gsmith1981

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Posted 12 November 2007 - 08:38 PM

how'd those go

#13 dta08

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Posted 13 November 2007 - 01:49 AM

yeah how did those go i am curious as well.

#14 MrMycelium

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Posted 13 November 2007 - 11:26 AM

Ok, my foaf let them freeze for 14 days now (2 weeks)

Today they are thawing out. tomarrow my foaf will knock up jars, pc them now.

#15 MrMycelium

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Posted 13 November 2007 - 11:30 AM

Here is a quick pic of the frozen syringes thawing.

http://mycotopia.net...=1&d=1194972611

Attached Thumbnails

  • Froozen-thaw.jpg


#16 prism

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Posted 13 November 2007 - 12:03 PM

I bet they are fine... I wonder if they are viable if this changes their longevity... Could be a huge experiment Mr. Myck. GOOD LUCK!:rasta:

#17 prism

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Posted 13 November 2007 - 12:04 PM

Its really cool how you can see the spores frozen in :amazed:the ice!

#18 MrMycelium

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Posted 13 November 2007 - 12:05 PM

I bet they are fine... I wonder if they are viable if this changes their longevity... Could be a huge experiment Mr. Myck. GOOD LUCK!:rasta:


hope so thanks

#19 EmitFitzhume

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Posted 13 November 2007 - 12:12 PM

The actual damage would occur at the cellular level when the water frozen in the cells bursts the cellular membrane. I wonder how spores react to freezing?

Here's a link with some info about the cryo-storage of spores. While this reference is for Microsporidia, I'm sure some basic principles could be applied to the cryo-storage of fungi spores. The mention of glycerol as a cryo-protectent would most likely prevent the cellular explosion due to the increased size of frozen water.

Storage of Spores

"Most terrestrial species can be stored frozen at -20 to -30° C in a household freezer. The inclusion of 50% glycerol in the suspension as a cryoprotectant is frequently required. Even under the best of conditions, repeated freezing and thawing causes spores to lose viability, therefore, spores should be store in small aliquots."

#20 MrMycelium

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Posted 13 November 2007 - 12:20 PM

Very informative link, what is small aliquots?

If i read it right, they should be ok, but each time you freeze they get less viable




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