Frozen Syringes II
Posted 31 October 2007 - 02:17 PM
My foaf redid the syringes he put in the freezer last night with better ones today.
1 B+, 1 Pan-Cambo, 1 Azure-Hammond
All of the syringes have 8ml of spore solotion not 10ml because of how much
liquid expands when frozen.
How long should he keep them in the freezer? 1 week, 2 weeks, 1 month.
Posted 31 October 2007 - 02:22 PM
If they aren't after a week they aren't after a month
Posted 31 October 2007 - 02:38 PM
Posted 31 October 2007 - 02:47 PM
Posted 31 October 2007 - 02:49 PM
yeah i froze mine by mistake and they didnt work... :(
This is why my foaf used different strains. Woodlovers, Poo lovers, for lovers. lol
Posted 31 October 2007 - 05:39 PM
This will be good to know and yeah I'd say a week is plenty.
Posted 31 October 2007 - 05:46 PM
I agree. One day or one week at 0 wouldn't change a lot. So IMO you may even try them tomorrow.
I would think after they are frozen solid all damage if any will have occurred. Try it for a week and see what grows. don't forget to leave room in the syringe for h2o expansion.
Posted 02 November 2007 - 07:35 AM
Posted 02 November 2007 - 11:21 AM
Like everyone says, a week should be good.
Will post pics today sometime of how things are going.
Posted 13 November 2007 - 11:26 AM
Today they are thawing out. tomarrow my foaf will knock up jars, pc them now.
Posted 13 November 2007 - 12:03 PM
Posted 13 November 2007 - 12:04 PM
Posted 13 November 2007 - 12:05 PM
I bet they are fine... I wonder if they are viable if this changes their longevity... Could be a huge experiment Mr. Myck. GOOD LUCK!:rasta:
hope so thanks
Posted 13 November 2007 - 12:12 PM
Here's a link with some info about the cryo-storage of spores. While this reference is for Microsporidia, I'm sure some basic principles could be applied to the cryo-storage of fungi spores. The mention of glycerol as a cryo-protectent would most likely prevent the cellular explosion due to the increased size of frozen water.
Storage of Spores
"Most terrestrial species can be stored frozen at -20 to -30° C in a household freezer. The inclusion of 50% glycerol in the suspension as a cryoprotectant is frequently required. Even under the best of conditions, repeated freezing and thawing causes spores to lose viability, therefore, spores should be store in small aliquots."
Posted 13 November 2007 - 12:20 PM
If i read it right, they should be ok, but each time you freeze they get less viable