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Frozen Syringes II


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35 replies to this topic

#21 EmitFitzhume

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Posted 13 November 2007 - 01:12 PM

I think "Small aliquots" means don't freeze a gallon worth of solution. 8ml syringes should be fine.

#22 MrMycelium

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Posted 13 November 2007 - 01:24 PM

I think "Small aliquots" means don't freeze a gallon worth of solution. 8ml syringes should be fine.



cool. thanks

#23 MrMycelium

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Posted 15 November 2007 - 12:53 PM

foaf knocked up 6 jars today.

2qt popcorn = b+
2qt popcorn/hpoo = pan cambo
2qt verm/brf/sawdust/wood chips = Azures

Will post pics later.

#24 prism

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Posted 15 November 2007 - 01:11 PM

Good vibes your way homie!!

#25 oeisa

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Posted 24 November 2007 - 10:40 PM

any updates?

#26 MrMycelium

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Posted 24 November 2007 - 10:43 PM

Several updates to post.

Sorry i have been out if town for the holidays.
Now that i am back i can get back on track.

I will post pix and more in the morning.

#27 Hippie3

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Posted 24 November 2007 - 10:57 PM

:cool:

#28 WookieSapian

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Posted 25 November 2007 - 10:49 AM

I would think just like anything else.

the spores are hydrated, they freeze, the cellular walls burst. which renders them useless.

#29 gsmith1981

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Posted 25 November 2007 - 11:02 AM

can't wait to see results hope this prolongs shelf life

#30 Hippie3

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Posted 25 November 2007 - 11:13 AM

well we already know that cubie spores cannot survive long freezes , the question is how long can they last.

#31 Doctor D

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Posted 25 November 2007 - 11:41 AM

Might not the spores fare better if they were frozen dry, rather than allowed to hydrate prior to freezing? The frozen water will be like shards of glass ripping through the spore.

If they were allowed to dry, perhaps with dessicant to sap out all bits of moisture, I posit that they would have a much greater chance of survival at freezing temperatures.

#32 Hippie3

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Posted 25 November 2007 - 12:05 PM

a plausible hypothesis
but then
those would not be syringes eh ?
that is the question before us...

#33 Doctor D

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Posted 25 November 2007 - 12:17 PM

a plausible hypothesis
but then
those would not be syringes eh ?
that is the question before us...


:lol: I never thought of the obvious. You're right, it wouldn't be a syringe.

#34 EmitFitzhume

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Posted 27 November 2007 - 11:21 AM

The actual damage would occur at the cellular level when the water frozen in the cells bursts the cellular membrane. I wonder how spores react to freezing?

Here's a link with some info about the cryo-storage of spores. While this reference is for Microsporidia, I'm sure some basic principles could be applied to the cryo-storage of fungi spores. The mention of glycerol as a cryo-protectent would most likely prevent the cellular explosion due to the increased size of frozen water.

Storage of Spores

"Most terrestrial species can be stored frozen at -20 to -30° C in a household freezer. The inclusion of 50% glycerol in the suspension as a cryoprotectant is frequently required. Even under the best of conditions, repeated freezing and thawing causes spores to lose viability, therefore, spores should be store in small aliquots."


Perhaps the freezing should be done again with a fresh specimen and a buffer solution. "The inclusion of 50% glycerol in the suspension as a cryoprotectant is frequently required."

#35 MrMycelium

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Posted 27 November 2007 - 01:11 PM

A update on the jars. All cooking at a humid 80F degrees.

2qt Popcorn = b+ (no growth yet. :cry:)

2qt popcorn/HPoo = pan cambo (Just starting to show mycelium in one jar. :eusa_pray)

2qt verm/brf/sawdust/wood chips = Azures (Got lots of mycelium growing, very strong. :eusa_danc)

Waiting on sony to send me a new usb cord for my camera. Puppy eat the old one.

Sorry all this is coming so slow, i dropped way behind on work because the holiday. Will be back on track soon.
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#36 hyphaenation

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Posted 27 November 2007 - 02:15 PM

Keep up the good works Mr. M :eusa_clap




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