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an observation about trich


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#1 Hippie3

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Posted 11 November 2007 - 08:28 AM

i've noticed that, on close examination,
almost all my incidents of trich involve
a hair that has fallen on the casing surface.
so i did an experiment-
i placed some wet coir in a bucket
and placed a few hairs on it.
sure enough, the normally very contam-resistant coir soon developed a patch of trich near one hair
and some cobweb mold near another.
so might i suggest that
folks should wear hair nets or shower caps, etc.
when working with their projects
and keep an eye out for any loose hairs.
:horse:

#2 mrpanda

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Posted 11 November 2007 - 08:32 AM

mmmm maybe thats why my contams are low. I don't have much hair and what I do have is locked in gel most of the time.

#3 camMyco

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Posted 11 November 2007 - 08:32 AM

Thanks, Hippie! I've always followed the belief it was from skin cells (learned from you) so hair would be a great source of Trichoderma.

#4 Hippie3

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Posted 11 November 2007 - 09:34 AM

whiskers, eye-lashes, arm hair, nose, etc.
all bad.
cover as much skin/body area as you can
[clean clothing, gloves, head cover, face mask, etc.].

#5 syvel

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Posted 11 November 2007 - 10:17 AM

Interesting.

#6 spacecake

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Posted 11 November 2007 - 11:22 AM

A shower before working with your mushies helps alot too !

#7 LotRev

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Posted 11 November 2007 - 11:46 AM

That is not good news, as my dog sheds to no end.

My own hair is hard enough to keep up with.

Every project done around this area with this dog around
has gone the way of trich.

#8 Hippie3

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Posted 11 November 2007 - 11:53 AM

cats too

#9 bugs

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Posted 11 November 2007 - 11:58 AM

Makes sense. Hair's hanging out in the breeze, and catches all the stuff flying around.

#10 Foster

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Posted 11 November 2007 - 12:06 PM

Good to know hip:bow:. Maybe i will order that tyvek body suit. Foster

#11 camMyco

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Posted 11 November 2007 - 05:55 PM

I guess ...Derma...should be the first clue.

#12 abbr.

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Posted 11 November 2007 - 10:24 PM

This past summer a friend and I
battled with trich, he has cats and dogs
at his house, and at my house,
Im constantly finding my hairs on
my pillows, hats, shirts, hmmm
Must be getting thin up there:eusa_booh
But stray hairs could def. have
been the source.
We could not narrow down
exactly where the trich was coming from.
Tried to figure out if it was the
straw, the h-poo, the casing materials,
our pasteurizing!
Racked our brains for days!
Hopefully this will make all the difference. :eusa_clap

#13 MrMycelium

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Posted 11 November 2007 - 10:28 PM

My hair is almost down to my ass, I am doomed. lol

#14 greenweanie

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Posted 12 November 2007 - 03:42 PM

thanks for the observation hippie. i should have noticed that right away :( my old house - no pets. the gf's house, dog and soon to be a couple of kittens. guess its time i make a larger glovebox for doing larger projects than basic bread tins :)

#15 twoguysupnorth

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Posted 12 November 2007 - 04:23 PM

thanks for the info. well there is no way around a glove box here i bet. a few pets and a dusty old house. i havnt even tried yet, but i was seriously concerned about the dust already. any tips? because there is no way around it.

#16 NEPHROSIS

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Posted 12 November 2007 - 04:33 PM

Good to know, glad I'm bald hehe.

#17 scientia

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Posted 12 November 2007 - 04:38 PM

I have two cats and I really don't have any problems with contams. The house isn't very clean either, and was probably built in the 1800's. It's all about being clean when you need to.

I use a glove box to do ALL innoculations, agar work, printing, everything. I also don't let my cats into the room where I keep my fruiting chambers, and still ozium it down before spawning anything. Also, a little bit of bleach in everything helps too.

In short, don't get rid of your animals/shave yourself. Those tyvek suits ARE surprisingly affordable though ;).

#18 hour

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Posted 12 November 2007 - 04:58 PM

Good to know, glad I'm bald hehe.


Yeah - atleast we have one reason to be happy about balding.

#19 bear

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Posted 14 November 2007 - 07:53 AM

Yeah.. good observation - it seems to agree with the general issues around here.

#20 myco-curious

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Posted 14 November 2007 - 02:40 PM

When inoculating, those are a lot of the same precautions I take. After setting up the environment and while allowing the dense fog of oust to thoroughly settle in the room undisturbed... I do the follow regime.

Turn off the HVAC unit for the house, throw a freshly laundered pair of scrubs into the dryer/tumbler and head off to take a shower. I shave, brush my teeth, use mouth-wash and get dressed with the still warm scrubs. I then pull my hair back into a tight-fitting cap, put on latex gloves as well as a set of new tyvek wrist sleeves. I then put on a surgical** mask (which is not the same as a dust mask) and a pair of safety glasses. I definitively look like a fool at this point...but I'm ready to enter my now (reasonably) sterilized inoculation environment.

---
That being said, once I throw a colonized tray into the fruiting chamber I've been thoroughly neglectful of any form of sterilization procedure - hell the damn thing sits right next to an old deep-freezer that's died on me several months prior, but have only had one case of trichoderma show up so far and that was out of this grow...and I would be damned if the transport into the chamber wasn't something like a hair or whatnot. :thumbup:

** dust masks are designed to protect you from inhaling rather large particles from the environment, surgical masks are designed to stop your exhaled breath from entering the environment and potentially contaminating a patient. They're not interchangeable...




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