52 replies to this topic

[abbr.]

Hello, I was going to wait to start this log,
but Im bored.
I am using Waylitjims Pan Cyan Tek....

http://mycotopia.net...n-cyan-tek.html

The trays were innoc. on 11-20
Pics were taken on 12-6
I used a different type of PP5 container than the tek,
but I think it will be OK. (see pics)
I also doubled the BRF since I was innoculating with
a multispore syringe.
Standard Tyvek filter patch on top of lid.
How do they look so far?
This first one has strong looking mycelium.......
http://mycotopia.net...=1&d=1197314861
http://mycotopia.net...=1&d=1197314861
This one has much weaker looking mycelium.......
http://mycotopia.net...=1&d=1197314861
http://mycotopia.net...=1&d=1197314861
You can see the pattern I used to innoc. in the first 2 pics.
I wonder why some innoc. points took off while
others did nothing?
These pics are of 2 of the 4 trays made.
A friend has the other 2 and says still no growth.
All were made with the same set of syringes from one print.

Attached Thumbnails

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[spacecake]

You can see the pattern I used to innoc. in the first 2 pics.
I wonder why some innoc. points took off while
others did nothing?

That's probably due to the fact that your using multispore...
Or maybe you didn't shake the syringe before inoculation...

Looks good,but I would watch that lower right innoculation spot on the first picture,looks like the beginning of contamination.
I could be wrong ,bcause pan mycelium can look very strange...especially with Cambo's !!!
Also the last picture looks like contamination to me,..but again I could be wrong ,because I can only see the top of the jar,and color could be off.

But lets see what other people think of it.

Good luck !

[abbr.]

The top of the tray is all I can see as well.
Its only about 2 inches deep. Its a tray from
a takeout food place, and the sides are black.
As far as shaking I always do that, I'm thinking
maybe one whole syringe didn't get spores since
the friend that has the other 2 is not getting growth.
And Im praying its not contam. Theres no funky stench,
and when you look real close, it does look like little
rhizomorphic mycelium. I just can't get a close
enough pic. Im using a phone camera.
I thought some of the innoc. points looked a
little weird too.
What part of the second pic
looks contam to you? The whole thing?
The mycelium on that tray is very thin compared to
the other tray. And Im color blind, but the mycelium
looks white to me, and when I look real close I
can see lil baby rhizos.
Thanks for taking a look Space Cake.
Im going to see if I can get a few better/closer pics.

[spacecake]

In the last picture I see only one ring of mycelium,and alot of times that means fast growing contamination.. mostly trich.

But as I said,Cambo is a strange strain ,it has tricked me alot of times,that's why I'm not growing this strain anymore.

So this is why I'm still 70% sure your doing fine..lol ,...well we will see in a few days right..!

[abbr.]

Camera phone sucks, the one tray with the weak mycelium
wouldn't get a better pic. Heres the other tray, still not much better...

http://mycotopia.net...=1&d=1197318953

http://mycotopia.net...=1&d=1197318953

http://mycotopia.net...=1&d=1197318953

oops, sorry bout that middle one

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[VaporizerJon]

kinda lookin like contams to me...sorry bro

[Foster]

Yep, Sorry Man, that is lookin kinda funky, I see green tint and maybe some purple lipstick mold. It is really hard to tell with the pics, but stop sniffin it, you dont wanna be inhaling contaminant spores.

So you innoculated the tray? Why is the lid off? If you cant see below the top layer, how do you know its fully colonized? Seems to me, if the top layer is showing growth in the same points you hit it with, the underside may not be any further along than what your seeing. Thats just an invitation for contams. If you use clear trays or jars, you'll have a better look at whats actually goin on.

peace Foster

[abbr.]

Well the lid was off for pictures, you can't see thru the
condensation. And as I said, there only about 2 inches
deep, I don't see how being clear would change anything
but the side pinning. O well, try again some other time.
Ill have someone who isn't color blind look today, I'm not
saying this tray isn't contam., but the shitty pics aren't helping.
Foster, Im not sure I understand what you are saying.
THe needle was stuck in about an inch when innoc., it
wasn' t just sprayed on top if thats what your asking?
Are you saying the bottom layers not being colonized
is an invitation to contams? I can see down the sides,
it is colonizing under there. I don't use clear trays
for cubes and its pretty easy to tell when its ready
to fruit, are pans that different?
IDK, thanks for your time guys.

[fedshtkpndrk]

If at all possible you should isolate the jar in question from your others, to be safe. Pan Myc can look odd, also whats your Temps? Heat can cause the myc to look odd too. Pan myc usually isn't very rhizo, but like Space said Pan Cambo myc has its own character. The overlay is looking good, has the jar been introduced to fruiting conditions?

I also doubled the BRF since I was innoculating with
a multispore syringe.

Normal 5:5:1 Waylit ratio works great for MS, I would hate for this to be the reason for the introduction of contams (If it is a contam). Im sending good fruiting vibes your way for fat stemmed Cambos. :loveeyes:

Best of luck,

feds

[fedshtkpndrk]

THe needle was stuck in about an inch when innoc., it
wasn' t just sprayed on top if thats what your asking?
Are you saying the bottom layers not being colonized
is an invitation to contams? I can see down the sides,
it is colonizing under there. I don't use clear trays
for cubes and its pretty easy to tell when its ready
to fruit, are pans that different?

I think Foster is trying to say that there is more benefit in verifying that the Spore Solution made its way to the bottom of the PP5 container. I think your bottom layers should be fine. If that is a verm layer I would scrape it off with a clean fork, and case it with 50/50 Cactus Soil/Verm. Mist really really well, and incubate for a couple more days. Pans aren't difficult, Yes they are different in that they require more attention. You'll nail it bro.

-feds

[abbr.]

"I remove the lid and use 3 or 4 inoculation points, with 6 cc's of LC.
The jars are fully colonized in 2 weeks time, and ready for phase II.
If you plan on using spores, I recommend adding a bit more BRF to
the formula. I believe Golly is trying equal parts manure, verm and BRF."

That is from waylits thread on Pan Cyan. I can't remember exactly how much
brf we used, but, I do think we doubled it.
You say the overlay looks good heh?
I thought that was the odd part!
These are at about 82* in an inc. dub tub thing.
They have not been introduced to fruiting conditions.
I have some Goliath coming, so Ill follow the tek exact for that one.
I prob. will get the jars like waylit used.
BTW these arent cased yet either, still waiting on sub. colo..
Thanks for the fat stem vibes Feds! :rasta:

[abbr.]

I think Foster is trying to say that there is more benefit in verifying that the Spore Solution made its way to the bottom of the PP5 container. I think your bottom layers should be fine. If that is a verm layer I would scrape it off with a clean fork, and case it with 50/50 Cactus Soil/Verm. Mist really really well, and incubate for a couple more days. Pans aren't difficult, Yes they are different in that they require more attention. You'll nail it bro.

-feds

what about the uncolonized spots, scrape down to colonized sub. then case?

[fedshtkpndrk]

If the cake is not completely colonized, then I'd leave it be until it is. If the cake is colonized I would scrape off the verm layer completely and apply the casing. Then follow the rest of Waylit's Tek.:)

There is a major benefit in having a see through container, Pans usually wont pin on the side if there is an area with good fae and rh to fruit from (i.e. the top) even if its exposed to light.

Cheers,

-feds

[fedshtkpndrk]

Overlay generally = Good Pinset with Pans

-feds

[abbr.]

Alright, Ill take a look at em after and see
whats going on in there.
Thanks, Feds

[Foster]

Right on feds. Sorry abbr, i wasnt clear on how you nocced em. Didnt know you removed the lid. Hopefully in a GB? Also, my mistake, the verm on top should be dry verm, not fc. I assumed it was already colonized, and you were casing with it because you said trays. Pans myc is very wispy, sometimes looks like cobweb mold, very strange stuff indeed. best o luck man

[abbr.]

Sorry for all the Q's, but do you think
using FC verm. is slowing the mycelium
from properly spreading?
Waylits tek is great, but theres a few
things in there that aren't specific.
The verm being dry, how much gypsum
and hydrated lime for the casing layer.
Maybe these things are common knowledge
and Im just a donkey.

[fedshtkpndrk]

I wouldn't worry about the gypsum. The lime can be added during pasteurization in small amounts, 1 Tsp is plenty. Waylit Pastuerizes in a waterbath inside a pillowcase, this is the method I use as well. Most Cactus Soil has a small amount of calcium carbonate anywho, so If you want to do without the lime you should be fine. :)

Field Capacity Verm is not recommended, as it has a tendency to harbor contams. It may be causing colonization to slow, air exchange isnt as good as dry verm to the sub. It may be beneficial to replace the verm layer in a GB. :thumbup:

-feds

[fedshtkpndrk]

Correction, Cactus Soil usually has Calcium Rock Phosphate among other things.

http://mycotopia.net...ns-img_1334.jpg

[abbr.]

I was wondering how I was going to
pasteurize the casing materials.
I will use the bath style.
Would mixing the verm , cactus mix
and lime before pasteurizing be good?
Or do you past. seperate?
Also, when it's done pasteurizing, cooling,
do you just squeeze it out to FC ?
And would FC be the same for exotics casings
as it is for cubes?

Sry for the noob Q's.
Thank you for your help.