I dug for this one in the archives and didn't see it. Thought it had been posted before. Anyway, take note this thing is oldskool. So, there are better methods now but this was what was being done a while back for cakes. Foaf, was running like 80-100 of these a week. they work. but grain to grain with bulk is better. however, if you just can't get the bulk down or grain, just give this a try. and make sure you keep them incubating in a very clean environment.
These were also called fat bags. it is no different than using any other bag but your using brf. downside, brf contams easy so sometimes if your not clean enough you may have problems. and if you transfer more than a few times. you ware out your mycelia.
Fat Bag Tek(Lazy Mofo Bag Tek)
first of all i want to say this tek is for newbs who have problems with grain or are too lazy. this is also for people who only have one pf jars worth of mycelia. 1 jar can easily turn into 100 in 2 weeks time. this basically is spawning with brf using ziplocs and no flowhood!!!!!!!
a foaf had 1 pf jar and turned it into 25 bags in 2 weeks. that is 100 jars worth of substrate from 1 pf jar. now, take into consideration g2g(grain to grain transfers) with the limitations of this tek. a friend did this for 2 months straight with no contamination, however the foaf would highly suggest after 2-3 transfers or 1 month renewing the mycelia from either multispore inoc or agar transfer to wbs or rye berry and then doing it again. now that or just use brf with multispore.
1. 1 colonized pf jar
2. 4 uncolonized but pced sterile pf jars.
3. 1 ziplock bag
4. 1 needle
1. clean your work area using alcohol and lysol. typically, wear a mask and gloves and sterilize the air with lysol as well as a hepa filter. take note this tek requires no touching of the substrate!
2. get 4 uncolonized jars and 1 colonized jar and turn them upside down and pat them until they are loose from the jar.
3. open lids and dump off excess verm layer then dump 4 uncolonized cakes into ziploc with colonized bag.( this needs to be done very quickly, less airtime the less chance for contams)
4. now seal ziploc and crunch up all cakes, making sure the colonized cake gets spread evenly throughout the bag. this way colonization takes place quickly.
5.LASTLY AND MOST IMPORTANTLY POKE HOLES USING A NEEDLE! use a small sewing needle so that a few holes are poked along the top of the ziploc. and MAKE SURE your incubation room is very sterile.
6. let incubate for 3-6 days and full colonization occurs. no shaking necessary.
included below are some pics. the first is 1 day after incubation, the second is 3 days after incubation. this is an incredible discovery in brf. it is a simple newb way of multiplying spawn ten fold in 2 weeks. all you have to do is keep making brf jars. and then renew inoculations once a month or every few weeks.
here is a pic a the next day after spawning
here are some pictures of the bags in effect! this is not a clone. just multispore.
Edited by eatyualive, 16 August 2014 - 08:08 PM.