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psilocybe cyanescens closeup


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#21 flipp diggity

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Posted 26 December 2007 - 09:06 AM

these shots are progressive
one of each layer removed
notice the air holes on pic one done with sewing need
one of the bins had trichoderma but it was removed and surrounding areas were spritzed with peroxide/water mix
http://mycotopia.net...=1&d=1198677560
http://mycotopia.net...=1&d=1198677560
http://mycotopia.net...=1&d=1198677560
http://mycotopia.net...=1&d=1198677560

#22 flipp diggity

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Posted 26 December 2007 - 09:12 AM

peeling yours apart can be risky because of contam but cardboard doesnt grow much contam directly
not that ive seen anyway
but if you do and want to accelerate growth you can flip the center layer to face new mycelium upward and it still will quickly reconnect to the already established network on the bottom
this will speed the colonization of the top layer giving me a few days

#23 flipp diggity

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Posted 26 December 2007 - 09:35 AM

primordial groth of other bins
check out this knot[ATTACH]71499[/ATTACH]

#24 abbr.

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Posted 26 December 2007 - 10:37 AM

Not sure if its my comp, but I can't view your attatchments.
Cool pics up top.

#25 flipp diggity

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Posted 26 December 2007 - 05:31 PM

:amazed::loveeyes:1.THIS IS A HYPHAL KNOT WERE TWO COMPATIBLE GROWTHS HAVE INTERTWINED
2.THIS IS ANOTHER PIN
3+4.THIS IS A KNOT THAT IS TRANSITIONING

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#26 flipp diggity

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Posted 26 December 2007 - 05:40 PM

im reposting the previous post wich had no thumbs

these shots are progressive
one of each layer removed
notice the air holes on pic one done with sewing need
one of the bins had trichoderma but it was removed and surrounding areas were spritzed with peroxide/water mix
check out the little droplets on the mycelium in last picture

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#27 Nzo

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Posted 26 December 2007 - 06:10 PM

please post end results.


is there a way to sterilize the cardboard after soaking it to
ensure its sterile?

#28 flipp diggity

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Posted 26 December 2007 - 06:35 PM

yes the water i soak it in is a peroxide/bleach/water mixture witch is active and bubbling when mixed 1part each bleach and peroxide per 15 parts water this is the same mixture i soak wood in and spritz on dry anything to maintain high humidity. this is nice because not only can most contaims enzymes utilize it, the h202 is pumpin extra oxygen wich the fungus thrives on
actually for one of my next projects im interested to see what adding pure oxygen to the fruiting chamber does

#29 flipp diggity

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Posted 26 December 2007 - 06:38 PM

also this treated cardboard method is a good way to work out contam
ive taken contam mycelium and transfered to cardboard which is cleaned and treated then when it transfers back to wood i had no problems

#30 flipp diggity

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Posted 26 December 2007 - 06:57 PM

there are two things
first once youre established
you will have byproduct
like substrate brooth (in this case alder chips, finch seed, and maple sawdust)the soup creating during soaking and straining substrate. which is soaked in the same mixture.
and also like old substrate which i just incubate
buth of these are below in thumbs

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#31 flipp diggity

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Posted 26 December 2007 - 07:15 PM

second you can treat and utilize both these products to boost growth
this works well when used to boost chips for cardboard to chip transfer
.first the broth is sterilized then allowed to cool
then i add a touch of peroxide to the brooth just for kicks
then i add that mixture to wood chips


then you will need to prepare your composted/myceliated mess
it now has a nice earthy/fungus odor
on a clean cutting board you chop muck into mush and spritz with treatment repeatedly while mixing
finally tip cutting board to drain using knife to keep mush

you then mix the chip blend with you super mush
and you have created your fruiting layer
note: only a small amount of muck was used this is a great nutrient/ interconnector.
by theory the more that the mycelium is rejoined the more knots, the more pins, well you get the point
but ive always used this sparingly because the chips are key and should make up most of the mix.
check out thumb link looking very closely you can actually see that those specks are atcually finely chopped mycelium
ps the liginous brooth is awesome and super pumped whith nutrient for fungus
great way to make the fungus well fed give it wood juice

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#32 flipp diggity

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Posted 26 December 2007 - 07:22 PM

this is what the fruiting substrate layer will look like
feel free to ask questions im here to share

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#33 flipp diggity

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Posted 26 December 2007 - 08:22 PM

high density mycelium just before adding chips
also there is a before and after wait period of corrugated cardboard
the last pic is of the composted material before chopping
by the way, just to clear things up when i say composted material i just mean old post fruited substrate that was stored in a 75`f room with some light and allowed to decompose for about 3 weeks adding excess water helps the material to decompose to a muck like state

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#34 flipp diggity

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Posted 26 December 2007 - 08:38 PM

this is moved below

#35 Nzo

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Posted 26 December 2007 - 08:43 PM

this is what the fruiting substrate layer will look like
feel free to ask questions im here to share


sorry im a bit confused..so did you inoculate those chips with the
soupy broth, or is that the same substrate with the cardboard.
so you used an old spent fruiting substrate (made of chips, wood,old wood mycelium, ect.)
chopped it up and made the broth? have you used this slime before, did it have some sort of results? it seems like a good idea..

#36 eatyualive

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Posted 26 December 2007 - 08:57 PM

very nice shots!:eusa_clap

#37 flipp diggity

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Posted 26 December 2007 - 09:00 PM

the broth comes from pre soaking chips/seeds/sawdust in water /peroxide/ bleach
the remaining water after straining is pored into jars and boiled 25 min
it then cools and is used for re soaking chips and spritzing existing

#38 Nzo

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Posted 26 December 2007 - 09:06 PM

i see what you mean, recycling water. cool.

#39 flipp diggity

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Posted 26 December 2007 - 09:13 PM

you lose a lot of nutrient thats water soluble
so reuse it
they love it

#40 flipp diggity

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Posted 26 December 2007 - 09:31 PM

the first pic
i took the fruiting substrate out of fruiting chamber
because it had pin formation but not enough and that run had no cardboard foundation.
i now have used it to top a cardboard foundation which was innoculated sandwich style
then an equal amount of fresh broth soaked super chips
and mixed vigorously breaking up myceliated material
close ups
there are some close ups of the chip surface so you can see the amount of broken mycelia that is present
this is good because network will become very dense
and is multi layered
cardboard/myc/cardboard/myc+chipsmix
last pic
the grass seed has sprouted it has survived the treatment
im interested to see how the symbiotic relationship between the grass and the mushrooms affect the characteristics of the fungus

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