Paradox
©
Fisana

Jump to content


Photo
* * * * * 2 votes

TV Guide to Colonizing a Quart Jar in Three Days


  • Please log in to reply
161 replies to this topic

#21 vinz

vinz

    mycotopiate

  • Expired Member
  • 1,160 posts

Posted 08 January 2008 - 08:59 AM

what an idea TV.. damn :)
we are lucky to have you here with us to share the things you come up with..
3 days is insane!
amazing :bow:

#22 eatyualive

eatyualive

    ExoCannibalist

  • Honorary Former Staff
  • 6,153 posts

Awards Bar:

Posted 08 January 2008 - 09:25 AM

Thanks for the good reviews! So what do y'all think we ought to call this thing?
This setup works well for making isolates, and I've used almost an entire stem as the source material, which I can do thanks to the sharp blades (just mix up the water with your favorite LC formula instead of plain). I peeled the outer layer off and dipped it into a dish of peroxide before dropping it into the blender jar, and the blades turned it to mush. It recovered and grew fast thanks to using the whole stem instead of just a little chunk, and I left the blades attached until it was ready to blend again just before using it to inoculate the next step. For air exchange, I had an airport stuck in the hole I'd drilled in the side of the jar. You can draw the isolate back up through the port or switch the blades for the dispenser lid. When making isolates, it's good to have a few redundant blender jars available as cloning is more challenging than blending the BRF cake.
One thing that's been bugging me for a long time is while I'm certain I'm not the guy who came up with using these blades on mason jars, I can't seem to remember or find the place I'd seen it described before (years ago), and so far I have not read of anyone around here using them. So, what's up with that? Anyone else out there know of someone using blender blades on jars for home mycology?


hey tv, this was used in 9er tek cloning method with an osterizer blender. it attaches a 1/2 pint jar rather easily. makes for good clones and lc's.

Mycotopia Web Archive: Cloning
Mycotopia Web Archive: Blender


you won't have to buy that many blenders. foaf has 4 blender assemblies. only and uses one blender to blend each one. just break your cake into 4. all you do is pc a few at a time if your doing large amounts. so 4 quart jars with blender assemblies attached with foil over them. then you can cut your cake into 4 pieces and use the same amount of water. diluting it will only make your slurry less chunky. i think 1-1/2 pint brf cake would easily do 4 times what your saying. especially in an lc.


none the less. this is a great writeup.

should we make a contest to see what to name it? how about "Tv's Mcelial Blast Tek"

i don't know just throwing something out there. foaf does something similar with lc's like this but doesn't use the brf cakes. and uses syringes
to inject quarts directly. but using brf cakes would pose problems for syringes unless you get the large gauge needles. and even then you sometimes get clogs.
also, foaf does something very similar to this with brf cakes but just uses a sterile fork to break up the cake and transfer it to each jar. however, after seeing this, ahhh foaf is going to use this from now on. 1+1=2. why didn't foaf put this together earlier lol? anyway, it seems to be more work constantly scraping the cake with a fork. however, since the colonized brf cake is already
in 1/2 pints, couldn't you also just cut the cake in half and pour sterile water into the 1/2 pint? i guess either way works, but you have more space with the larger jar attachment and get more liquid culture this way. im really loving this man! can't wait to put it into action tommorow with some pan mycelia. oh yeah eat's got a cool new project to do thanks to the TVcasualty!
:headbang::headbang::headbang:

one more thing id like to add. i see you have a second lid with something bolted into it. this is for pouring?
foaf would actually leave that step out and pour directly from the jar. im betting you tried this and it
was a mess huh? but you would be able to use all the slurry.

what happens is the lid to the blade assembly is just laid flat across the top and then transferred directly into
the jars right after the blending takes place so it is done all in one sitting. poured kinda like a g2g transfer. in
between each liquid to grain transfer, foaf would lay the blender assembly flat across only opening it slightly
per transfer. this can be done with one hand, you just have to get used to it. this makes it one big step and
if you prep everything right, you just did all your work in one sitting. can't beat that!

storage or quick use:
and if you are storing this, how long would a brf cake slurry last in a jar would you say? ive had brf cakes last at
least a year and grow out even if they were gooey or not so solid. and quarts can last even longer. but would one
be skeptical about using brf stored in liquid like this for a longer period of time.? im not really sure how long it would last so this is something to throw into the mix. i would say if you were going to do this, and store it, you may want to keep it only a week or so. but unlike fahts mycelia syringe tek, the jar isn't dried out. i think with grain, the grain would recover once the liquid is removed and last a very long time. but with this tek, i can't see the brf cakes lasting that long pulverized in liquid. so in laymans terms, would this be a week long storage with this lid or would one be able to store it longer.



ah ok i see, its for pouring. neat! and cool idea. foaf is gonna try this with blade assembly lid only and see if it works. im sure there can be a million variations of this. very very nice!

#23 Guest_floppypeter_*

Guest_floppypeter_*
  • Guest

Posted 08 January 2008 - 11:39 AM

very nice write up !

You make it look easy !

#24 Godflesh

Godflesh

    Mycotopiate

  • OG VIP
  • 628 posts

Posted 08 January 2008 - 12:58 PM

:loveeyes::bow::loveeyes:

#25 hyphaenation

hyphaenation

    Former Staff

  • Honorary Former Staff
  • 13,977 posts

Donator


Awards Bar:

Posted 08 January 2008 - 01:34 PM

This is plain out fantastic !

:bow:

I can't wait to try this with "edible" species.

Thanks TV-C

:heart:

#26 TVCasualty

TVCasualty

    Embrace Your Damage

  • Moderator
  • 13,971 posts

Awards Bar:

Posted 08 January 2008 - 08:00 PM

Wow, thanks for digging up the links and for your thoughts. I have to shut my computer down in a minute (tornadoes approaching, lightning, etc.) but wanted to say that the nozzle dispenser allows a precise metering of the exact same amount of slurry into each quart, which is how to get them all to finish at the same time (that's more important when you're only incubating for three days).

I'll be back when the coast is clear to discuss some of the other points y'all have brought up, and I really appreciate the feedback from everyone, thanks!

P.S.- Use the metal mason jar band, not the original plastic one that came with the blender. Mine melted.

#27 eatyualive

eatyualive

    ExoCannibalist

  • Honorary Former Staff
  • 6,153 posts

Awards Bar:

Posted 09 January 2008 - 09:58 AM

Wow, thanks for digging up the links and for your thoughts. I have to shut my computer down in a minute (tornadoes approaching, lightning, etc.) but wanted to say that the nozzle dispenser allows a precise metering of the exact same amount of slurry into each quart, which is how to get them all to finish at the same time (that's more important when you're only incubating for three days).

I'll be back when the coast is clear to discuss some of the other points y'all have brought up, and I really appreciate the feedback from everyone, thanks!

P.S.- Use the metal mason jar band, not the original plastic one that came with the blender. Mine melted.


tv, easy way to solve this problem. foaf only pcs the metal blade assembly and the rubber ring. it is laid flat on top of a regular mouth 1/2 pint or quart. foil is then wrapped over that and pced. when everything is cool and sterile, the plastic assembly is rubbed down heavily with rubbing alcohol and that is all. this is because the plastic mount for the pc is not touching anything sterile. it will only touch the exterior of your jar. you then blend and remove the assembly. when you g2g you would then just slightly open up the metal blade assembly and pour.

i love the idea of the measurements, its great when you have a certain # of jars you need to knock up. as for just colonizing quickly, me thinks the pouring method should workout if your only doing a few jars just for speed. and i like the statement above that you directed at the jars that the jars were ready, but you weren't ready.:headbang: always a good thing!

want to hear a funny story? foaf did that and pced the plastic assembly with the blade assembly. well when it was cooled and removed from the pc, it was melted to the jar. foaf ended up tossing the jar about 2 stories down to the concrete and broke the glass jar to get the plastic lid off. It actually worked, and the assembly was reused. But the assembly was only pced for 15 psi for 20 minutes. foaf had to learn the hard way, and we won't even go into the blowing up the microwave story....

luckily, it works. any longer and it may have been permanently attached.

that was one of those "doh" moments!

good luck and hope your safe with those tornados.

#28 kocos

kocos

    The PsYhead

  • Expired Member
  • 2,641 posts

Posted 09 January 2008 - 10:12 AM

Great wrightup man!!:bow:

#29 TVCasualty

TVCasualty

    Embrace Your Damage

  • Moderator
  • 13,971 posts

Awards Bar:

Posted 09 January 2008 - 11:13 AM

tv, easy way to solve this problem. foaf only pcs the metal blade assembly and the rubber ring. it is laid flat on top of a regular mouth 1/2 pint or quart. foil is then wrapped over that and pced. when everything is cool and sterile, the plastic assembly is rubbed down heavily with rubbing alcohol and that is all. this is because the plastic mount for the pc is not touching anything sterile. it will only touch the exterior of your jar. you then blend and remove the assembly. when you g2g you would then just slightly open up the metal blade assembly and pour.


I think the way I do it requires the metal bands, since I like to make a few blenders and stack them in the PC. The band helps hold it all together, and then each blender jar has it's own, sterile band. In a residential context, my philosophy is the more sterility the better! And it's not like I don't have an endless supply of metal bands laying around...

i love the idea of the measurements, its great when you have a certain # of jars you need to knock up. as for just colonizing quickly, me thinks the pouring method should workout if your only doing a few jars just for speed.


Yes, that's a good point. For a couple of jars, it's not that critical. In fact, this might not be the best way to do only a couple of jars (more effort than necessary). The method I posted took me awhile to develop, and I got it to the point where I thought it was ready to share when I'd managed to go from 12cc's of spores to 6 BRF half pints to 120 quarts of colonized grain in 19 days (I was trying to get a huge amount of spawn to make an outdoor bed of edibles; the armadillos had other ideas however so now I'm building an armadillo blender :nunchuck:). The nozzle allowed the liquid to be precisely estimated so I never ran out too early or under-spawned some jars, and I used a separate blender/nozzle assembly for each of the 6 half-pints for cleanliness.

It took me slightly less than two hours to dump the half-pints into the blenders, blend, and inoculate all 120 quarts (and that includes the time to shake the hell out of them).

As to storage, I really don't know how long the liquid would keep; might be worth some experiments. I'd imagine it would last longer by adding a small amount of peroxide to it before putting it in the fridge or whatever (extra oxygen), and I suppose freezing it might kill it (but is that certain??) though the thought of frozen spawncubes is appealing. Maybe propylene glycol (the less-toxic version of antifreeze found in Mountain Dew and other "food" products) could be added to or in lieu of water, and then it could be frozen without killing it (preventing ice crystal formation). Ahh, more experiments...


I'm intrigued about your pressure dispensing idea, and it occurred to me that once you plunge the air syringe to pour a shot, you'd need to pull the tubing off and suck more air into the syringe to push the next shot out. This air would need to be filtered or else it's a contamination vector. What if, instead of air, you used a mini co2 injector, like this one.


I was thinking about using a rather large syringe to minimize or eliminate the need to refill it with air, but ultimately I'm leaning towards an air compressor. The CO2 things might work if there was a way to precisely meter the flow, since for filling tires and staying affordable I bet the inflator would still blast way too much CO2 out even if set to the minimum. I'm also not absolutely certain that they are sterile, or what the effect might be of introducing 100% bone-dry CO2 to a liquid culture. I suppose you could make one with a piece of dry ice in a plastic bottle (though NOT sealed!!) and a regulator that switches between venting to the air and feeding to the dispenser; as the ice sublimates it creates a positive-pressure flow of CO2.

Since air compressor accessories are common and compatible, I'd been looking into using one of those "air carry" tanks that you can fill at gas stations. By attaching a hose with a pressure regulator and a foot switch, then adding an in-line micron filter, I think I can make a setup that will allow me to dispense one precise shot each time I hit the foot pedal (freeing a hand). That would be the decadent luxury model, of course. It would also allow use of a dispensing needle on the end of some silicone tubing (autoclavable) since the air pressure would likely blow out chunks that would normally clog the needle. Then, you can just use rubber or silicone ports to inject the slurry instead of pouring it. That is a major part of my idea for developing a spore-to-fruiting method that requires no glovebox or flow hood and produces large quantities of spawn very quickly, but no time and low funds are slowing me down.

#30 the jesus

the jesus

    Mycotopiate

  • Expired Member
  • 2,583 posts

Posted 09 January 2008 - 11:47 AM

Phenomenal TV. :bow:

#31 hyphaenation

hyphaenation

    Former Staff

  • Honorary Former Staff
  • 13,977 posts

Donator


Awards Bar:

Posted 09 January 2008 - 12:02 PM

TVC presents :

Slurry-In-A-Hurry

I made my rig yesterday as soon as I read this thread ! I'm trying it out this morning with King Trumpet Oyster. If it goes well I have another batch of WBS ready that I want to try falbino slurry on.

Can 100 % colonized grain be used to make S-I-A-H ? If so yeeeehaw :rasta:

thanks TVC

much appreciated

H

#32 Dank Side Of The Shroom

Dank Side Of The Shroom

    VIP Member

  • Expired Member
  • 3,447 posts

Posted 09 January 2008 - 01:58 PM

holy shit that is awesome.

#33 hyphaenation

hyphaenation

    Former Staff

  • Honorary Former Staff
  • 13,977 posts

Donator


Awards Bar:

Posted 10 January 2008 - 03:42 PM

I've been thinking about this thread for a couple of days since I first saw it. Last night I did all the above with a king trumpet oyster cake and made a nice slurry. I used the slurry to inoculate jars of WBS. To compensate for the extra moisture I put a good layer of verm on the bottom of each jar.

So far so good. Its really easy to use this tek.

Also I tried to do this with PF albino on rye grain. Problem was that even with a small amount of water the grains don't blend well, they shoot up and stay basically whole. After a few grinding whirls the water is covered in mycelial strands. This made the liquid quite runny but it still worked good.

Can't wait to see how these go in the next few days.

#34 eatyualive

eatyualive

    ExoCannibalist

  • Honorary Former Staff
  • 6,153 posts

Awards Bar:

Posted 10 January 2008 - 07:53 PM

Slurry-In-A-Hurry


now that is a good one!

Can 100 % colonized grain be used to make S-I-A-H ? If so yeeeehaw


i bet you can and i don't see why not! hehe something new to try.

#35 TVCasualty

TVCasualty

    Embrace Your Damage

  • Moderator
  • 13,971 posts

Awards Bar:

Posted 11 January 2008 - 11:01 AM

I've been thinking about this thread for a couple of days since I first saw it. Last night I did all the above with a king trumpet oyster cake and made a nice slurry. I used the slurry to inoculate jars of WBS...

Also I tried to do this with PF albino on rye grain.


That's awesome, I'm looking forward to hearing about both, especially the whole grain since I've not tried that before. I'd guess that 100% millet would be a great whole-grain spawn for blending, since even if the grain didn't break up it's still small enough to fit through the dispenser, though poring directly from the jar is always an option. I try to avoid open pouring because of the vastly increased surface area of the slurry exposed to air vs. the exposure from pouring through the nozzle.

Slurry-In-A-Hurry


Nice one! I should've thought of that since there's an Indian restaurant in my town called "Curry in a Hurry." Of course, eating there can sometimes result in an entirely different kind of "slurry in a hurry," so I'd hate for anyone to get confused... :lol:

#36 eatyualive

eatyualive

    ExoCannibalist

  • Honorary Former Staff
  • 6,153 posts

Awards Bar:

Posted 12 January 2008 - 01:31 PM

tv,

this was done using basically the same steps as above but with the use of a shmuvbox instead of a flowhood. and there was no drilling done. the blade assembly was used as a lid and 1/10th of a pint of solution was used per jar. the rest of the leftover solution was placed in the fridge to see what happens....(betting its going to turn colors).

all of the slurry was poured even the chunks. everything was just poured like a glass of water poured into other glasses of water only they were quarts filled with wbs grain.

it was shaken and it appeared to be a bit gooey but the chunks of the brf/verm slurry were sticking to the sides of the grain jars.


this was going to be done with pan mycelia but some cubes needed to be done atm. some more pans will be tried very soon and will keep you posted.

the slurry was used liberally and hopefully this will make it colonize even faster. if not, then we may see contams. this was all done very casually in semi sterile conditions without the use of a flowhood. lets hope for the best.

total prep time for materials before g2g. 20 mins
total time it took to transfer 10 mins
very quick!

Attached Thumbnails

  • IMG_4477.JPG
  • IMG_4477.JPG


#37 mycobri

mycobri

    Stained Blue

  • Honorary Former Staff
  • 3,508 posts

Posted 12 January 2008 - 01:55 PM

Most Amazing Work TV :bow:
gonna have to try this out sometime

#38 waylitjim

waylitjim

    A Mirror Image

  • Honorary Former Staff
  • 4,706 posts

Awards Bar:

Posted 12 January 2008 - 02:20 PM

Very creative TV.
I'm glad you shared this one with us.
The evolution of cultivation just took another step forward.
  • shakid likes this

#39 hyphaenation

hyphaenation

    Former Staff

  • Honorary Former Staff
  • 13,977 posts

Donator


Awards Bar:

Posted 12 January 2008 - 06:00 PM

Would albino be a racial slur?

#40 eatyualive

eatyualive

    ExoCannibalist

  • Honorary Former Staff
  • 6,153 posts

Awards Bar:

Posted 12 January 2008 - 06:09 PM

lol.....

Would albino be a racial slur?






Like Mycotopia? Become a member today!