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should i make an agar transfer?


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#1 Nzo

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Posted 04 January 2008 - 06:33 PM

well i have a few dishes of Syzygy although its only a bit
rhizomorphic mostly fluffly mycelium. i previously tried an agar transfer
with a plate like this and it failed..my guess is that its because
it is mostly fluffy and not rhizomorphic..OR
because it was too cold and they werent incubating.
should i give it try to wbs?
this dish photo is kinda old and know the dish in completely white
and some rhizos growing up the jar

#2 Nzo

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Posted 04 January 2008 - 06:41 PM

here we go

should i try it even though its not rhizomorphic?

Attached Thumbnails

  • syzygy 013.JPG


#3 ShroomGuerilla

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Posted 04 January 2008 - 06:54 PM

word:pirate:

#4 Hippie3

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Posted 04 January 2008 - 07:38 PM

just take a few tiny pieces, smaller = better for transfer
right now it's all mixed together,
tiny piece = fewer substrains
which will spred and distinguish over next few transfers

#5 Nzo

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Posted 04 January 2008 - 07:40 PM

wow great idea hippie.
transfer a good amount of small pieces then throw
em in grain.
and i DO need to make some agar2agar transfers
in hope to isolate a more rhizomorphic dish
thanks

#6 Hippie3

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Posted 04 January 2008 - 07:42 PM

i meant transfer each tiny piece into its' own agar plate-
as it grows out it will sector-
split into areas with distinct appearances
each is a substrain, select a rhizo one
and transfer that to new plate to grow.
once properly isolated into a pure rhizo substrain
you should see if it pins well,
this too can be observed on agar
before going big to grain.

#7 Nzo

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Posted 04 January 2008 - 07:42 PM

oh okay,
thanks for the tip hip! :rasta:

EDIT
wow crazy i didnt know different sectors were substrains! thanks for all the info
one thing im not sure of, how do i check
"if it pins well" on agar before going on grain? that would
be really helpful.

#8 BuckarooBanzai

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Posted 04 January 2008 - 11:22 PM

Just an opinion: rhizo doesn't mean instant success and fluffy doesn't mean instant failure.

Personally, I've tended at this point to lean towards whatever sector grows the fastest - rhizomorphic qualities don't really mean that much to me any more.

I've seen "fluff" fruit to beat the band and almost ropey rhizo perform poorly.

Opinion: whatever grows fastest in your environment will probably give you the best chance for good results. Again, personal opinion only.

#9 Hippie3

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Posted 05 January 2008 - 08:27 AM

how do i check
"if it pins well" on agar before going on grain?


same as pinning anwhere else,
you expose the plates to light then wait, watch.



cf. https://mycotopia.ne...7-agar-pinning/


Edited by Sidestreet, 14 August 2015 - 10:42 AM.
Fixed link


#10 vinz

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Posted 05 January 2008 - 09:56 AM

isolating or cloning on h202 agar / peroxide agar helps against contams a lot.. makes it so easy..

#11 aumbrellaforainydays

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Posted 05 January 2008 - 05:30 PM

whats that ratio for h202 / peroxide in agar?

#12 vinz

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Posted 06 January 2008 - 09:43 AM

just squirt it a few drops
make sure the agar is not too hot
let it cool to be touchable..

#13 Myc

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Posted 06 January 2008 - 10:53 AM

whats that ratio for h202 / peroxide in agar?


Counterculture was advising me that 7cc in 1 liter of agar is the right ratio.
I haven't tried this. Any thoughts?

#14 Hippie3

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Posted 06 January 2008 - 11:05 AM

6 or 7 is right,
cf. Mycotopia Web Archive: making peroxidated-agar

peroxidated agar<

#15 Lazlo

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Posted 06 January 2008 - 12:07 PM

You can also use a q-tip dipped in the h202 to apply a layer of it to the dry agar. The dry agar will suck the h202 in. That way you know for a fact it's there and will be effective as well.

Make sure to dip the entire q-tip in h202 so it's good and sterile while working with it over the agar. Don't have a dry end of the q-tip that you're holding in other words. Dip both ends of it so the whole q-tip is saturated.

#16 Hippie3

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Posted 06 January 2008 - 12:14 PM

nice 'tip'
:bow:

#17 Myc

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Posted 06 January 2008 - 12:23 PM

I was wondering about "spot application".
Since h2o2 degrades @ 140*F, one would have to pour the entire liter of agar at once with the 7cc per liter idea. Reheating left-over agar to pour later would destroy the h2o2.

#18 Hippie3

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Posted 06 January 2008 - 12:35 PM

correct. one adds the h2o2 once it's cooled down before it gels up.

#19 Sloppy Joe

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Posted 28 February 2008 - 03:43 AM

me being new to this, i was really impressed to see the mini-pins on the agar. would you ever eat any of the mini-pins considering the rest of the plate was ok?
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