hello mycos, i thought i'd take a minute to share my progress with agar. At first I tried germinating plates from syringes and got no success. Then i found myself with a B+ print (:bow:), which i cut a tiny corner off of with some alcohol rubbed scissors and placed spores-down on a plate. This grew out in about a week, though there were two spots of contamination. I didn't get any pics of this first plate, and now it's been tossed, but the contaminated spots were small and pretty far from the myc. I transfered to two new plates, and both looked clean and healthy. I'm now on the third transfer, and finally took a minute to get some pics. So, here they are:
I think i'm starting to be able to see some sectoring in the third xfer dishes, though it's kind of hard to tell where the sector boundaries are. Any tips on that would be sweet. The dish with three cultures is neat, as you can clearly see the zones of inhibition, where incompatible strains meet. The idea is to take a small piece from the center of each strain, at the edge of the myc so as to get the most vigorous growth. Right? Put each on its own dish and grow out, repeat until there's no more sectoring. Then transfer to grain and fruit each isolate until i get a keeper.
I have decided that agar is really fun! I like being able to watch the cultures spread and interact, and I feel like I'm developing a more intimate understanding of the mycelium. I'll try to keep this thread updated as things develop.
Edited by Sidestreet, 14 August 2015 - 10:45 AM.