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#21 elgr

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Posted 20 December 2004 - 12:44 AM

Yeah, the actual cultivation of ergot is generally out of the area of the chemists anyway. Its really all about having a source of ET. The general idea is that it comes from overseas, countries where its less controlled. Of course, I'm sure there are exceptions/corruptness closer to home, but who knows. Not to mention that when Pickard was busted, his source of ET was cut off as well. The person who narced him out knew that very well, and even kept a kilo of ET from the authorities he worked with for a long time after. They knew he had it, and eventually he gave in and gave it back.



#22 mycojay

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Posted 21 December 2004 - 12:30 AM

todd skinner did not sem to be his source of ET but rather a unfortuate acquaintance or maybe a small part of the finacial side. He did however provide a front with his supposed spring plant cover.

#23 Guest_pbeester_*

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Posted 21 December 2004 - 01:13 PM

If you're growing ergot, you also need a strain that is high in alkaloids. Used to have a strain at JLF but I don't know if they're still in business. ET from the pills is the best way to go, cause growing ergot would be a pain in the ass.

#24 Guest_burnt_*

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Posted 22 December 2004 - 03:47 PM

i did some reading once on pills with ET most of them had only a few milligrams or less of ET. so youd need lots of pills to get a sizable amount of ET. but i guess it depends what source your getting it from.

#25 elgr

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Posted 24 December 2004 - 01:15 AM

JLF still has it.. but they were busted a few years ago for something. Surely not safe to order from.

No, skinner wasn't his source, but he was fairly involved. But, I believe his provided A LOT on the financial side. Of course, the money wasn't his own. His business 'cover' was not only that, but a lie as to where all these wonderful funds are coming from.


#26 Guest_sweetness_*

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Posted 24 December 2004 - 10:43 AM

http://mycotopia.net...tml ?1103901692

#27 Guest_roo_*

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Posted 24 December 2004 - 01:13 PM

"Hey Roo would you happen to know a link where I could read about this "liquid tek"
thanks"

It is used in the pharmo industry. There was some work done using GM fungi recently.

http://www.world-of-...t_alkaloids.htm

Dont try this at home...

#3: Ergot culture


NOTE: contact with ergot compounds can be dangerous. Only after a
basic understanding of the techniques employed in the handling of
dangerous or poisonous organisms is reached should one proceed with
the culture of ergot.

The need for absolute sterility cannot be overstressed. Consult
any elementary text on bacteriology for the correct equipment and
procedures. Avoid prolonged contact with ergot compounds, as they
are poisonous and can be fatal.


A) Get a source for Claviceps Purpurea fungus


If no source can be found, you can make a field trip to obtain
it from rye or other cereal grasses. Rye grass is the best
choice. The ergot will appear as a blackish growth on the
tops of the rye where the seeds are. They are approximately
the same shape as the seeds and are referred to as "heads" or
"ergot". From these heads or ergot sprout the Claviceps
Purpurea fungi.

They have long stems and bulbous heads when viewed under a
strong glass or microscope. It is these that must be removed
from the ergot, free from contamination, and used to inoculate
the culture material.


B) Make a culture medium


Combine the following ingredients in about 500 ml distilled
water in a 2 L small-neck flask:


Sucrose 100 g

Chick pea meal 50 g
Calcium nitrate 1 g
Ca(NO3)2
Monopotassium phosphate 0.25 g
KH2PO4
Magnesium sulphate 0.25 g
MgSO4
Potassium chloride 0.125 g
KCl
Ferrous sulphate heptahydrate 8.34 mg
FeSO47H20
Zinc sulphate heptahydrate 3.44 mg
ZnSO47H20


Add water to make up one liter

Adjust to pH 4 with ammonia solution and citric acid

Sterilize by autoclaving


C) Make a culture


Inoculate the sterilized medium with Claviceps Purpurea under
sterile conditions, stopper with sterilized cotton and
incubate for two weeks, periodically testing and maintaining
pH 4. After two weeks a surface culture can be seen on the
medium. Large-scale production of the fungus can now begin.


D) Large-scale production


Obtain several ordinary 1 gallon jugs.

Place a two-hole stopper in the necks of the jugs.

Fit a short (6 inch) tube in one hole, leaving two inches
above the stopper. Fit a short rubber tube to this. Fill a
small (500 ml) Erlenmeyer flask with a dilute solution of
sodium hypochlorite (NaClO). Extend a glass tube from the
rubber so the end is immersed in the hypochlorite.

Fit a long glass tube in the other stopper hole. It must
reach near the bottom of the jug and have about two inches
showing above the stopper. Attach a rubber tube to the glass
tube and fit a short glass tube to the end of the rubber tube.


Fill a large glass tube (1" x 6") with sterile cotton and fit
one-hole stoppers in the ends. Fit the small glass tube in
the end of the rubber tube into one stopper of the large tube.
Fit another small glass tube into the other stopper. A rubber
tube is connected to this and attached to small air pump
(obtained from a tropical fish store).

With this aeration equipment you can assure a supply of clean
air to the Claviceps Purpurea fungus while maintaining a
sterile environment inside the solution.

Dismantle the aerators. Place all the glass tubes, rubber
tubes, stoppers and cotton in a paper bag, seal tightly with
wire staples and sterilize in an autoclave.

Fill the 1-gallon jugs 2/3 to 3/4 full with the culture medium
and autoclave.

While these things are being sterilized, homogenize in a
blender the culture already obtained and use it to inoculate
the material in the gallon jugs. The blender must be sterile.


EVERYTHING must be sterile.


Assemble the aerators. Start the pumps. A slow bubbling in
each jug will provide enough oxygen to the cultures. A single
pump may be connected to several filters.

Let everything sit at room temperature (25 C) in a dark place
(never expose ergot alkaloids to bright light - they will
decompose) for a period of ten days.

After ten days, adjust the culture to 1% ethanol using 95%
ethanol under sterile conditions. Maintain growth for another
two weeks.


E) Extract ergot alkaloids


After a total of 24 days growth period, the culture should be
considered mature. Make the culture acidic with tartaric acid
and homogenize in a blender for one hour.

Adjust to pH 9 with ammonium hydroxide and extract with
benzene or chloroform/iso-butanol mixture.

Extract again with alcoholic tartaric acid and evaporate in a
vacuum to dryness.

The dry material is the salt (the tartaric acid salt, the
tartrate) of the ergot alkaloids, and is stored in this form
because the free basic material is too unstable and decomposes
readily in the presence of light, heat, moisture, and air.

To recover the free base for extraction of the amide or
synthesis to LSD, make the tartrate basic with ammonia to pH
9, extract with chloroform, and evaporate in vacuo.





#4: Synthesis of LSD from ergot alkaloids or LSA

(including sections on isomerization, separation,
purification & crystallization)


NOTE: the chemicals and reactions described below are potentially
dangerous even to an organic chemist in a well-equipped laboratory.

The publishers therefore disclaim responsibility for any damage or
injury resulting from the improper handling of the chemicals and
techniques described, and strongly urge all persons unqualified to
perform the reactions to use instead the comparatively easier,
safer ergot culture and LSA extraction process.


A) Synthesis of LSD
(iso- & dextro-lysergic acid diethylamide)


PREPARATORY: obtain one red and one yellow photographic safety
light and one weak, long-wave ultraviolet light. These are used to
prevent the hydrolysis of lysergic acid compounds.

NOTE: Aluminum foil must be used to cover the chemicals when light
is present. Rubber gloves must be worn; these compounds are
extremely poisonous.

[The source implies but does not state that one may replace
"ergot alkaloid" in the following with the seed-derived semi-
pure LSA concentrate from #2. --Ed.]


USING YELLOW LIGHT:

Place one volume of ergot alkaloid in a small roundbottom
flask. Add 2 volumes of anhydrous hydrazine and reflux for 30
minutes, or the mixture may be heated in a sealed tube at 112
Celsius for 30 minutes. If the reflux technique is used,
maintain atmospheric pressure by using an open container or
fractionating column.

After heating/refluxing, add 1.5 volumes of water to the
mixture and boil gently for 15 minutes. After boiling is
complete, cool the mixture in a refrigerator until
solidification. The solid material obtained is iso-lysergic
acid hydrazide.

USING RED LIGHT:

Chill all chemicals (reagents) to be used to 0 Celsius. Place
an open flask in an ice bath. Add 100 ml concentrated
hydrochloric acid (chilled to 0 C).

Quickly add 2.82 g of the lysergic acid hydrazide to the
hydrochloric acid, being careful to maintain a temperature of
0 Celsius.

Add 100 ml of a 0.1 N (1/10th Normal) solution of sodium
nitrite (chilled to 0 C) and stir vigorously for 3 minutes.

Continue stirring at 0 Celsius and add dropwise 130 ml of the
hydrochloric acid.

When the acid addition is complete, continue stirring for 5
minutes, then neutralize the solution with sodium bicarbonate,
using a saturated water solution of the bicarbonate.

Extract the solution with ether, remove the water layer, and
dissolve the gummy substance in ether. Add this to the ether
layer.

Add 3 g of diethylamine for every 30 ml of the ether extract.

Let this stand in the dark, and gradually warm up to 20
Celsius for at least 24 hours.

Evaporate this solution in a vacuum.

The material remaining is a mixture of the inactive
iso-lysergic acid diethylamide and the active lysergic acid
diethylamide (LSD-25). The inactive isomer must now be
converted (isomerized) to the active isomer to greatly
increase the yield, since the inactive compound predominates
in this synthesis.



B) Isomerization of iso-LSD into the active LSD-25


USING THE RED LIGHT:

Dissolve the synthesized material into the minimum amount of
ethyl alcohol.

Mix a 4 Normal solution of potassium hydroxide in ethanol.
The amount of solution needed is twice the volume of the
iso-LSD/ethanol solution.

Add the two solutions together and let the mixture sit for 4
hours at room temperature.

Neutralize the mixture with dilute hydrochloric acid, then
make it slightly basic with ammonium hydroxide.

Extract the mixture with chloroform, sparate the chloroform
layer, and extract this four times with a 25% volume of water.

Evaporate the chloroform in a vacuum. Discard the water
extracts. The material left after evaporation a mixture of
iso-LSD and LSD-25, the active LSD predominating.

The mixture may now be separated by chromatography and the
iso-LSD again isomerized by the above process.



C) Separation, purification & crystallization of LSD-25


USING A DARKROOM:

The material obtained from the isomerization process is now
dissolved in a solution prepared from 3 parts benzene/1 part
chloroform. Use 50 ml solvent per 1 gram LSD material.

Mix a slurry basic alumina in benzene. Pack it into a 1 inch
chromatoghraphy column until it fills 6 inches.

When the slurry settles, drain the benzene/chloroform down to
the level of the basic alumina, and carefully add an equal
amount of the LSD/solvent solution.


USING A WEAK, LONG-WAVE ULTRAVIOLET LIGHT:
(to follow the blue band only)

Drain the solution through the column. The fastest-moving,
blue fluorescent band contains the LSD-25. Collect this
fraction and evaporate in a vacuum. The syrup remaining will
crystallize spontaneously, but slowly. Do not heat.

Use the UV light only whe necessary to follow the blue band in
order to avoid decomposition of the compounds.

Dissolve the syrup or crystal in tartaric acid solution and
recrystallize to form the stable end-product (dextro lysergic
acid diethylamide tartrate).

The material remaining in the column may be removed with
methanol, evaporated in a vacuum, and recycled through the
isomerization and subsequent procedures by itself or combined
with fresh material.
Also, all leftover solutions and residues may be neutralized
with socium bicarbonate, evaporated in vacuo, and extracted
with ammoniacal chloroform, the extract evaporated to dryness,
and the residue reused.





#5: Preparation of lysergic acid from the amide


NOTE: this synthesis is as difficult and dangerous as the rest, and
is of use only if using one of the following two LSD synthesis
methods, which require lysergic acid as the starting compound. The
lysergic acid amide obtained from the extract of ergot or seeds
need not be converted to the acid prior to its use in the synthesis
of LSD providing that the synthesis used is #4 given above, and
giving the starting material "ergot alkaloid".


Dissolve 10 g lysergic acid amide in 200 ml methanolic
potassium hydroxide solution.

Remove the methanol by vacuum as soon as the amide is
dissolved.

Dissolve the residue which is left into 200 ml of an 8%
solution of potassium hydroxide in water.

Heat this mixture on a steam bath for 1 hour.

Pass a steam of nitrogen gas through the flask during the
heating process. (The ammonia which is evolved in the gas
stream may be titrated with hydrochloric acid in order to
follow the reaction.)

Neutralize the mixture with tartaric acid (neutral to congo
red) and run it through a filter paper.

Extract the mixture with ether in a separatory funnel. Save
the water layer, discard the ether layer.

Filter the solution through a filter paper and evaporate.

Upon evaporation, dry crystals of lysergic acid will be
obtained.




#6: Synthesis of LSD
using lysergic acid
the quickest way to make pure LSD-25
PREPARATORY: see #4

NOTE: The chemicals and techniques described are potentially
dangerous. It is highly recommended that the physical and chemical
properties of the reagents used be studied by those persons
unfamiliar with them before the synthesis is attempted.



USING THE YELLOW LIGHT:

5.36 g of d-lysergic acid are suspended in 125 ml
acetonitrile, and the suspension is cooled to about -20
Celsius in a bath of acetone cooled with dry ice.

To the suspension is added a cold (-20 C) solution of 8.82 g
of trifluoracetic anhydride in 75 ml acetonitrile. The
mixture is allowed to stand at -20 C for about 1 1/2 (one and
one-half) hours.

(During this time the suspended material dissolves and the
d-lysergic acid id converted to the mixed anhydride of
lysergic and trifluoracetic acids.)

The mixed anhydride can be separated in the form of an oil by
evaporating the solvent in vacuo at a temperature below about
0 Celsius.

Everything must be kept anhydrous.


USING THE RED LIGHT:

The solution of mixed anhydrides in acetonitrile from above is
added to 150 ml of acetonitrile containing 7.6 g of
diethylamine.

The mixture is held in the dark at room temperature for about
2 hours.

The acetonitrile is evaporated in vacuo, leaving a residue of
LSD-25 plus impurities.

The residue is dissolved in 150 ml of chloroform and 20 ml of
ice water.

The chloroform layer is removed and the aqueous layer is
extracted with several portions of chloroform. The chloroform
portions are are combined and, in turn, washed with four 50 ml
portions of ice-cold water.

The chloroform solution is then dried over anhydrous sodium
sulfate and evaporated in vacuo.

NOTE: following the completion of this synthesis, follow the
procedures described for separation, purification, and
crystallization of LSD-25. If a higher yield is desired, follow
the procedure on isomerization after doing the separation,
purification, and crystallization.




#7: Synthesis of LSD
using lysergic acid
high-yielding and fast


PREPARATORY: see #4

NOTE: The chemicals and techniques described are potentially
dangerous. It is highly recommended that the physical and chemical
properties of the reagents used be studied by those persons
unfamiliar with them before the synthesis is attempted.

NOTE: the following procedure gives good yield and is very fast,
with little iso-lysergic acid being produced. However, the
stoichiometry must be exact or yields will drop


USING WHITE LIGHT:

Sulfur trioxide is produced in an anhydrous state by carefully
decomposing anhydrous ferric sulfate at approximately 480
Celsius. Store under anhydrous conditions.

USING WHITE LIGHT:

A carefully-dried 22 liter RB flask fitted with an ice bath,
dropping funnel, and mechanical stirrer is charged with 10 to
11 liters of dimethylformamide (freshly distilled under
reduced pressure).

The condenser and dropping funnel are both protected against
atmospheric moisture.

2 lb. of sulfur trioxide (Sulfan B) are introduced dropwise,
very cautiously with stirring, during 4 to 5 hours. The
temperature is kept at 0-5 Celsius throughout the addition.

After the addition is complete, the mixture is stirred for 1
to 2 hours until some separated crystalline sulfur trioxide-
dimethylformamide complex has dissolved.

The reagent is transferred to an air-tight automatic pipette
for convenient dispensing, and kept in the cold. Although the
reagent, which is colorless, may change to yellow and red, its
efficiency remains unimpaired for three to four months in cold
storage.

An aliquot is dissolved in water and titrated with standard
NaOH to a phenolphthalein end point.


USING RED LIGHT:

A solution of 7.15 g of d-lysergic acid monohydrate (25 mmol)
and 1.06 g of lithium hydroxide hydrate (25 mmol) in 200 L of
MeOH is prepared.

The solvent is distilled on the steam bath under reduced
pressure.

The residue of glass-like lithium lysergate is dissolved in
400 ml of anhydrous dimethyl formamide.

From this solution, about 200 ml of the dimethyl formamide is
distilled off at 15mm pressure through a 12-inch helices
packed column.

The resulting anhydrous solution of lithium lysergate left
behind is cooled to 0 Celsius and, with stirring, treated
rapidly with 500 ml of SO3DMF solution (1.00 Molar).

The mixture is stirred in the cold for 10 minutes and then
9.14 g (125.0 mmol) of diethylamine is added.

The stirring and cooling are continued for 10 minutes longer,
when 400 ml of water is added to decompose the reaction
complex.

After mixing thoroughly, 200 ml of saturated aqueous saline
solution is added. The amide product is isolated by repeated
extraction with 500 ml portions of ethylene dichloride.

The combined extract is dried and then concentrated to a syrup
under reduced pressure. Do not heat the syrup during
concentration. The LSD may crystallize out, but the crystals
and the mother liquor may be chromatographed according to the
instructions in the synthesis of LSD #4.


#28 Guest_roo_*

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Posted 24 December 2004 - 01:17 PM

This is just for information!! These things WILL kill you if you are not carefull. These compounds can be very easly absorbed by the skin, and lungs. If you try this and have no experiance dealing with such things you will die...

#29 Guest_JT_*

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Posted 18 January 2005 - 08:22 PM

i might get crackin on that someday

#30 Guest_filamentous_*

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Posted 27 January 2005 - 12:42 PM

It seems to me that the old peptide coupling link on the-hive.ws would be relevant to this. Morning glory and HBWR are easy extractions like the dmt extraction(identical - the anhydrous nature is different. If not going by this method then by fungi. There are liquid medias to grow fungi but there are some small things to consider. Cook the sugars seperately, and the micros seperately. The basification of ergot to retrieve the LSmonohydrate wouldn't be hard to do(look at the dmt extraction). The DEA can be gotten from hydrolizing DEET bugspray and a still would purify that. Other than the solvent to be used i.e. POCl3 those precursores are easy to acquire. Led's under red spectrum are photo compatible so you have your light. They also have blacklights in led. On to the glass. EBAY is the way it has been done before(pickard used it to send to a friend who covered as saying they were extracting essential oils from plants) Anhydrous means No water meaning that you need nitrogen or argon(colored) preferrable, to keep the reaction from exploding. From there you need a sep funnel and blacklight for the post reflux condensation procedure. Get the faster flourescing zone, take it and pruify with methanol and activated charcoal. Wash and repeat. Dry the extract. LSD. Basify the other bands in the sep to revert racemers back. Did I forget anything? This if for the bees...We miss the-hivePosted Image

#31 Guest_JT_*

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Posted 27 January 2005 - 02:09 PM

damn it i need a partner and a book.

#32 meihoe

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Posted 07 December 2004 - 10:17 PM

Does anyone have any cool pics of LSD "fluff" or any other interesting LSD pics? Man, do I miss that stuff, it used to be SOOOOOO plentiful now nothing.Posted Image

#33 Guest_ferry_*

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Posted 08 December 2004 - 02:16 PM

Yes,I'm missing it too !
I had some big pieces of blotter in my collection,but I don't now were I put the pictures of it !
The cheapest and greatest drug I had ever had !!!
Posted Image

#34 perrch01

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Posted 08 December 2004 - 04:15 PM

I only found good lsd once, and have been on a never ending quest for it since, and so far have gotten bunk product more than oncePosted Image

#35 Guest_grasshopper_*

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Posted 08 December 2004 - 06:27 PM

heres some pics.
http://www.erowid.or...d_images.sht ml

#36 bertha

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Posted 08 December 2004 - 06:59 PM

https://www.tripatourium.com/

#37 elgr

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Posted 08 December 2004 - 10:31 PM

Its making a pretty significant comeback. Especially within the past month or so. Many say its the best they've seen in over 2 years.

#38 perrch01

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Posted 08 December 2004 - 11:09 PM

How come there can't be any in my neck of the woods? Back in my old college town I found it once but its been nothing since, even at Dead and Phish shows!Posted Image

#39 Guest_texasbob_*

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Posted 08 December 2004 - 11:23 PM

I have seen some in my area lately,
Liquid

#40 perrch01

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Posted 09 December 2004 - 12:03 AM

I must live in the black hole of LSD it seems to be everywhere except for where I happen to be at any given timePosted Image

lol




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