
Bag is pulled thorugh a polypropylene ring (cut from a big syringe)

Bag is folded back...

...and secured with two rubber bands

The opening is stuffed with polyfill.

And this is covered with aluminium foil to prevent it from getting wet in the pressure cooker.

Bags after autoclaving (2 1/2 hours)

These bags should be inoculated with liquid mycelium since they cannot be shaken.