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H2O2 dunk?


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#1 Digi7ech

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Posted 27 April 2005 - 09:27 PM

Hi,
Been learning a lot from the forum.

My FOAF is on their second flush of BRF cakes and they've noticed a very small gold colored patch on one cake. The little patch had little hair like structures with a little bulb like end. Their assuming it's a yeast or some contam.

They was wondering if doing a H2O2 dunk would have any beneft.
I was trying to search and read that H2O2 would only kill spores.
So at the least it might have kiled the spores and left hopefully a dying patch.

My foaf doesn't have bleach so they're trying to get as good as they can with what they have.

Would a 10% dunk for 30-1hour do anything? Maybe a %20 mix? Extend time length?

Would an over night dunk in this hurt the mycelium?

Thx

#2 shocktherapy

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Posted 27 April 2005 - 09:36 PM

I'm not sure if what your describing is contamination or possibly pin development, your description of a "gold colored patch with a bulb like end" sounds alot like primordia formation and not contams, i'd have to see a pic to be sure though...how far along are your jars, like what percentage colonization, if your at 95-100% i'd say your having pins develop usually contams don't attack the mycellium directly they attack the uncolonized substrate thats why sterilization before innoculation is so important.

post a pic and then i'm sure someone will be able to give you an accurate prognosis of your situation.

#3 shocktherapy

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Posted 27 April 2005 - 09:37 PM

and if it is a contam then an h202 dunk wouldn't do much good because h202 only kills the spores and if it is a mould the spores will have already germinated making a dunk useless.

#4 Digi7ech

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Posted 27 April 2005 - 09:39 PM

The spot which is possibly a contam is the side of an eraser tip.

The little bulbs are almost microscopic. The bulbs on the ends are a little bigger than a pin tip.

The cakes are 100% colonized and just finished their second flush. They have some blue bruising but otherwise are healthy and seem to be contam free.

#5 taoistshredder

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Posted 27 April 2005 - 09:43 PM

"Would an over night dunk in this hurt the mycelium?"

No, but it may hurt any pins if it knocks them around too much. What you describe could be a contam, a pinset, or "mycellial piss;" hard to tell without pictures. I would isolate it and see what develops.

#6 shocktherapy

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Posted 27 April 2005 - 09:50 PM

is the "contam" a liquid or a solid?

#7 Digi7ech

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Posted 27 April 2005 - 10:33 PM

It was a solid. I isolated it and touched it and it wiped off like a powder.

#8 shocktherapy

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Posted 27 April 2005 - 11:09 PM

sounds like metobolic waste, or what taoistshredder calls "mycellial piss"

i can't be sure without pics though, try to get some and then we'll be able to tell ya.

#9 altered_states

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Posted 28 April 2005 - 09:55 AM

mycellial piss


lol
That's what I call it too; I'm very easily amused.

Peace
AS

BTW...
IMHO... Spend 50 cents on bleach or ask a neighbor/friend... Whatever.
I bet you have enough change in your couch to buy a gallon.




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