Innoculating LC (Liquid Culture)
Posted 26 February 2008 - 07:55 AM
He also has one extra syringe of mulitspore and one more jar of Hippies dextrose water and wonders if they would be best stored separately, or should he innoculate the second LC jar. He will have so much LC from the first jar he just wonders the best way to store the rest and keep it good as long as possible. thank you
Posted 26 February 2008 - 08:47 AM
- Heirloom likes this
Posted 26 February 2008 - 04:41 PM
Posted 26 February 2008 - 07:39 PM
what about the storage of the extra jar and extra syringe of multispoire? Better to innoculate the second jar and store like that ( 2nd jar wont be needed for a LONG time with the frist jar of LC ) or to keep them stored separate and innoculate the LC jar further down the road when it is needed?
Posted 26 February 2008 - 08:30 PM
Posted 26 February 2008 - 11:06 PM
Try cutting a piece of a brand new sponge about 3/4 inch square and a little longer than the length of your needle. Sterilize the syringe and needle with the sponge on it. After being sterilized, wet the sponge with alcohol so its damp. You have to have some sort of port on your LC jar to push the sponge and needle against to draw up the LC. Leave the sponge on to inoculate your PF style jars, the verm barrier will catch any alcohol, but this shouldn't be an issue if you haven't saturated the sponge. Grain jars need to have the sponge pulled back before inoculating since there is no verm barrier. This works really well if you have had a lot of contam issues and no glove box. Your jars have to have holes drilled in them however if you do not have a glove box..
So I just want to clarify, because I am trying to picture this in my head. My FOAF can have this sheath made from a sponge he keeps over the needle thoughout the ENTIRE injection process? I mean he withdraws the LC with this needle that is encased in an alcohol soaked sponge, then for shooting PF jars, he keeps this same sponge over the needle and then does NOT flame in between jars?
Posted 26 February 2008 - 11:20 PM
Posted 27 February 2008 - 06:35 AM
well that is an awesome idea1 Thank you! I wonder why this idea isn't in the archives or something???
Yep, thats your ticket hon. Keep in mind you only want it damp, not saturated. If you get too much alcohol in the sponge, squeeze out the excess. No flaming the needle required.
Posted 15 March 2008 - 01:35 AM