Clone Selection and Propagation of Agar Media for DummiesI started out in this hobby enamored with cloning, curious as to the process and then turned off by the level of detail and sterility that conventional techniques demand. I dislike glove boxes a lot, I cant stand wearing gloves while working either. I wanted to find a cloning tek that was simple and could be done in open air, although it seems like the Holy Grail. I was sent a cloning tek by Livewire420 and was asked if I could check it out, I immediately fell in love with the tek and tried it out. This is a syringe coring tek, just like a biopsy. Slow as we go, I’m going to stuff this thread with detail. I will do my best to document the process in real time, so bear with me :).
Days 1-21 Were spent preparing spawn and substrates Inoculating with multispore LC or Spore Solution and allowing for colonization (many examples can be seen here in the Mycotopia Web Archive: Grow Logs or Mushroom Photo Gallery & Grow Logs - Mycotopia Web Forums . Strains are PF Albino and Penis Envy both from Sporeworks.
Substrates were late cased with a 60/40 Verm/Coir casing layer. In the next few days we will begin to see the MS fruits developing, We will decide which fruits to clone based on several parameters, pinning speed, thickness, length, and overall aesthetics. I will place all Syringe core clones on antibiotic agar in ½ pint jars. All clones will be done open air, I will expect a couple to fail as I will be trying to work the camera at the same time. I have had an 85 percent or so success rate overall with learning curves using this tek.
I hope I can help the peeps out there who cant afford a flowhood, or are simple like me and dont have room for any more equipment :)