String Bean Agar
Posted 15 March 2008 - 11:58 AM
It simply amazes me the crazy things one can use to prepare agar formulae.
I've always had this mental picture of some scientist in a lab with all these complex chemicals and apparatus. Boy was I wrong!
String bean agar has three ingredients:
Dry powdered agar
Fresh string beans
Take 1/2 lb of string beans and load into blender
Pour in 500 ml of boiling water
Liquify (run blender for about 3-5 min.)
Strain (I use a "permanent" coffee filter made of wire screen)
Take this liquid and add water to make 500 ml again
Place in a pot
Bring to a boil
Add 10 g dry powdered agar
PC and pour.
I use an airport jar (thanks Hippie3), a 60cc syringe w/ a 14 ga needle.
I also have done a "taste test" comparison by placing live mycelial tissue (Pleurotus Ostreatus - Oyster) on the following:
MEA - Malt Extract Agar (Light) from Sporeworks
PDYA - Potato Dextrose Yeast Agar (home made)
SBA - String Bean Agar (home made)
I wanted to see how the various media compared to each other. I am quite happy since this is my first attempt at preparing my own agar formulae rather than using pre-mixed stock. Sorry for the poor pic quality.
Posted 18 March 2008 - 05:32 PM
I noticed the cubes enjoy Wheat Malt Extract Agar.
Thinking of trying some manure agar soon,
I think making my own agar is more fun than pre-mixed.
But I also have pre-mixed PDA, its nice to just weigh and cook if you're pressed for time.
Also, how do you like the sectored plates compared
to a regular? I noticed you have different media in each sector.
Using the airport and syringe method makes that fairly easy I'd assume.
Hope to see more pics in the future!
Posted 18 March 2008 - 08:55 PM
I actually ordered 300 regular plates and the company sent me 500 of these by mistake for the same price. PM me if you wanna try some as it seems I have a lifetime supply. :lol:
Attached is a photo of the same plate taken 4 days after the initial photo. It colonized in three days and I've since made transfers to other plates. Included are photos of the transfers after 24 hours. Oyster mycelium is amazingly aggressive.
Posted 18 March 2008 - 09:12 PM
[Cenmed Brand Lab Petri Dishes -ed.]
The plates were the wrong ones but they were cool about giving them to me for the lesser price that I paid for the single dishes.
I buy sleeves of single plates and other lab supplies from a local surplus dealer.
Edited by Sidestreet, 14 August 2015 - 10:09 AM.
Bad link removed
Posted 18 March 2008 - 10:43 PM