Posted 25 March 2008 - 07:44 PM
Hopefully this will serve as a meaningful contribution to this site. I have learned so much here I'm hoping I can give something practical and useful back.
- Tenderfoot likes this
Posted 25 March 2008 - 07:48 PM
Live tissue was transferred to Red Winter Wheat Berries and allowed to colonize.
G2G transfers were done to Wild Bird Seed and finally to Rye Berries.
Incubation temps. were between 67*F and 84*F.
Posted 25 March 2008 - 07:52 PM
Are you going to fruit them on rye? AFAIK it's a wood lover.
Posted 25 March 2008 - 08:02 PM
Fresh cut cottonwood log
The hardwood log was run through a jointer to produce a combination of wood shavings and sawdust.
The dry wood shavings were weighed to determine the amount of wheat bran to add for supplementation.
Supplementation was done with a ratio of 4:1 woodshavings: wheat bran
The block consists of 640g of woodshavings and 160g of wheat bran.
The wood shavings were hydrated to field capacity with a short soak. When using fresh cut lumber, the shavings are very near field capacity to begin with. I found soaking may have leached out important nutrients so I started another block which I misted with the water left over from the initial soak rather than soaking it.
Mix the dry wheat bran with the soaked woodshavings.
Load into autoclaveable spawn bags.
Secure top with binder clip.
Protect bag with old t-shirt (may be unnecessary?).
Load bag into PC with filter patch facing up and unobstructed yet protected from taking on moisture.
PC for 90 min. @ 15 PSI
Spawn with one quart of colonized grain spawn in glovebox or flowhood.
Seal bag with tape or impulse sealer.
Knead grain spawn into sterilized, supplemented woodshavings.
Shape substrate into the form of a block.
Posted 25 March 2008 - 08:05 PM
Posted 25 March 2008 - 08:26 PM
Since I'm growing from a tissue clone I suppose that the end result will prove which method is better.
The block on the left was soaked and the block on the right was misted and spawned at a later date.
Posted 25 March 2008 - 08:30 PM
I remember you brilliant Ps. Weilii grow and I'm sure you're gonna score jackpot yet again!
Sounds and looks really good!
Posted 25 March 2008 - 08:42 PM
I've started cultivating edibles and novelty species exclusively. Since edibles teks are kinda scarce I thought I'd post my experiment here.
In the works are:
Ganoderma Lucidum (Reishi)
Pleurotus Ostreatus (Oyster)
and Portabello (sp?)
They are all currently on agar and I hope to post growlogs for each one this year.
Posted 25 March 2008 - 10:47 PM
The blocks seem to go through distinct stages. Fully colonized, then with age they form a bubble like armor that is biege at first then darker brown. You watch for boil like primordia to form, perhaps a dozen then you cold water soak it for 24 hours and take it in to the fruiter, cut the bag etc.
Looking forward to seeing your results.
Here's a couple pics from my first ever block.
Posted 26 March 2008 - 01:10 PM
Posted 22 April 2008 - 08:05 PM
Ironically, the bag wich was spawned latest (3/16/08 - four days after the first bag) is showing the primordia formation.
Both blocks appear as though they've been rolled in popcorn.
The block which is forming primordia still has some areas which aren't covered with "bubble armor" - (to use Hyphae's description).
In all, I'm very happy with the results of this experiment.
I suppose I'll let several more primordia form accross the surface of the block before I dunk and induce fruiting.
This experiment gives me great optimism toward my other wood-loving gourmet and novelty species grows which are currently on agar plates just waiting to be tested.
Hoping for Panelus Stipticus "glow-in-the-dark" sawdust blocks for Halloween. I imagine the kids
will love them.
Just went back and looked @ your photos carefully. Was that block brown colored prior to dunking or after? I may be pre-mature in my presumption that what I'm looking at are primordia.
LOL Like a kid at Christmas......
Posted 23 April 2008 - 09:02 PM
Look carefully on those "bubbles" as more of them are likely primordia. Yours will go brown with age also in time. Maybe you have a very fast fruiting strain , and due to a bit of neglect tech and being slower mine got browner before the soak.
Best of luck
Posted 24 April 2008 - 09:16 AM
I'm satisfied that they are pins.
Don't know what strain this is since I got the tissue culture syringes as part of a trade. Labeling on the syringe looks consistent with Sporeworks methods so I'm assuming that's where they're from.
May wait a few days to dunk just to let some more pop out.
I'm hoping you can direct me to a tek you posted here awhile back.
It involves a piping system for adapting an ultra-sonic humidifier to a Martha's closet style greenhouse setup.
Can't remember the thread name.
Much appreciated if you could save me some scrounging.
WooHoo! Shiitakes with dinner soon!!
Posted 04 May 2008 - 05:21 PM
I waited until May 1st to soak the blocks.
They were immersed in a 10% peroxide solution bath and lots of ice cubes in a cooler which I cleaned and sanitized for the purpose.
I chose to dunk the blocks for 72 hours with the bags left on adding ice daily in order to keep the temps in the cooler as low as possible.
Water entered through the filter patches and served to further hydrate the blocks.
They were pretty heavy when the bags were cut away before placement into the greenhouse.
RH is being maintained between 85% - 90%
Temps. are around 69*-71*F
FAE 15 min. cycles four times a day
24 hours later and things are already getting started!
The soaked block now appears to be outperforming the "misted" block since it has more large pins. I suppose the proof will be in the final tally of the yeilds per block.
Posted 05 May 2008 - 10:54 AM
love, peace, compassion and respect
Posted 05 May 2008 - 11:19 AM
120v. You'll need to supply your own cord.
Plugged right into a standard 120v lamp timer.
Greenhouse volume is 31.5 cu. ft. (L x W x H)
So @ 65cfm (rating of the fan), air can be totally exchanged in less than one minute.
This fan is overkill for this setup. I'm famous for using leftover parts from other projects. Just something I've had lying around for a few years.....finally put to good use.
BTW, fan was mounted to a piece of leftover plexi-glass. The hole was cut using a roto-zip tool which is about the only effective way to work with plexi-glass.