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how to mix antibiotic agar?


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#1 teesus

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Posted 06 April 2008 - 08:05 AM

hello mycologists!

i have some dirty prints from the wild and want to clean them up on antibiotical agar. i got a few gram of streptomycin-sulfat, but i dont know witch ratio i should use in the agar and i wonder if i could pc the agar with the antibiotica or if i have to sterilize the streptomycin-solution with a syringefilter and put it on the plates after they where pc-ed.
if so do you know if i can pc syringe-filters that were used before? thanks everybody!

#2 Lazlo

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Posted 06 April 2008 - 09:18 AM

900ml's agar/100mg's of streptomycin sulfate. It is rather sensitive to heat from what I gather, so you'll have to filter it and add it right before the agar starts to harden.

#3 teesus

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Posted 06 April 2008 - 09:30 AM

thanks lazlo! i will do so. :bow:hope i can reuse the filters.

#4 Hippie3

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Posted 06 April 2008 - 09:32 AM

https://mycotopia.ne...96-antibiotics/


Edited by Sidestreet, 14 August 2015 - 10:26 AM.
fixed link


#5 teesus

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Posted 06 April 2008 - 10:04 AM

thank you hippie! interesting thread. i´ll try to organize some gentamycin next week, sounds less risky to put that stuff into the agar before sterilisation,
but i´ll try the streptomycin as well.

#6 teesus

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Posted 06 April 2008 - 10:22 AM

this are the syringe-filters i want to use. someone ripped open the packing , not sterile anymore.....

IMG_0706.jpg

do you know if these filters can survive the PC ?




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