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Pan Goliath Invitro


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#1 feralronin

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Posted 11 May 2008 - 04:07 PM

So I have 3 pints of Goliath that just became fully colonized about a few days ago and went to take to FC today when I found this. I didn't know pans could pin at all invitro so I had to share a pic. Sorry for the quality will get a better one up. You can see 2 tiny ones forming.

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#2 HUMBLE STUDENT

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Posted 11 May 2008 - 04:10 PM

what substrate mix did you use? if you put manure in it I wouldn't see why it wouldnt pin invitro but I'm a noob when it comes to pans so get more input

#3 feralronin

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Posted 11 May 2008 - 04:13 PM

the sub is a black cow brand compost/ verm/ brf. I think it was your thread H.S. that you were wondering about it and people wanted you to put up a pick but then said they thought it was a cube not a pan. I just didn't see much of it.

#4 fedshtkpndrk

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Posted 11 May 2008 - 08:19 PM

It is true, aggresive Pan strains will grow invitro. They will do so also on waylit's Pan cyan tek, Enjoy them :)

-feds

#5 feralronin

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Posted 12 May 2008 - 11:24 AM

The thing is that they were not intended to be done invitro. I was using Waylit Pan Tek but forgot to put on the cactus mix on in the beginning. For some reason I thought that when fully colonized I removed the verm layer and then applied the cactus/verm mix.
What I decided to try was to remove the verm layer and case with cactus/verm mix. Covered with foil and put back into the incubator for a few days. This is just my first time working with pans and they are quite different than cubies. Very different mycelium but very cool.
I took another pint that was finished and crumbled it into a small plastic container, then cased with cactus/verm mix. I just hope I can figure this out.

#6 abbr.

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Posted 13 May 2008 - 08:06 PM

The thing is that they were not intended to be done invitro. I was using Waylit Pan Tek but forgot to put on the cactus mix on in the beginning. For some reason I thought that when fully colonized I removed the verm layer and then applied the cactus/verm mix.
What I decided to try was to remove the verm layer and case with cactus/verm mix. Covered with foil and put back into the incubator for a few days.


You were correct in how you did it.
When you say "In the beginning", do you mean
at the time of innoculation?

When your tray is fully colonized, you scrape
the verm. barrier off and case.
Then send it back to the incubator for a few days.

At least that's how I understood the tek when I used it.

I'm glad this strain is working well for you. :thumbup:

#7 waylitjim

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Posted 13 May 2008 - 08:09 PM

Just posted a few photos in another thread.

http://mycotopia.net...html#post528430

#8 Hippie3

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Posted 13 May 2008 - 08:21 PM

http://mycotopia.net...8430-post3.html

#9 feralronin

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Posted 15 May 2008 - 10:22 AM

Ok so I became really confused for some reason using this tek. I ended up doing it correctly. I thought I mixed up the verm barrier step with the casing step. Duh. They are in the FC but look quite unusual for a pan strain. Will have to see how they develop.

#10 Hippie3

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Posted 15 May 2008 - 10:55 AM

another newer pic ?

#11 feralronin

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Posted 15 May 2008 - 10:57 AM

Will get one up later today I hope.

#12 Godflesh

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Posted 15 May 2008 - 01:32 PM

Hmmm, wander where those spores came from?:eusa_thin:eusa_eh:

#13 Workman

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Posted 15 May 2008 - 01:49 PM

The picture of the pins isn't very good but the yellowish coloration of the stem points to cubensis.

#14 abbr.

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Posted 15 May 2008 - 08:50 PM

Hmmm, wander where those spores came from?:eusa_thin:eusa_eh:



From me, why?

#15 feralronin

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Posted 16 May 2008 - 12:18 PM

Here is what I pulled today. No doubt cubes. Veil, tannish stems, and just plain huge. I wonder what strain it is??

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#16 feralronin

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Posted 16 May 2008 - 12:22 PM

Oh and just for clarification, cake was removed from jar and placed into the FC just like a PF cake, except I used a pp5 takeout container and cased the cake on sides and top with cactus/verm mix. Was hoping that the mushies that were pinning would do better, being these were going to be my first pan fruits and I wanted them to be as big as possible and there was almost no room for growth in the jar

#17 Godflesh

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Posted 19 May 2008 - 10:14 AM

The picture of the pins isn't very good but the yellowish coloration of the stem points to cubensis.



BINGO was his name o.

#18 feralronin

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Posted 19 May 2008 - 10:22 AM

Yeah these look a little different. :rasta: Cubes were pinning invitro. http://mycotopia.net...html#post530738




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