prevent condensation in petri dishes
Posted 19 March 2006 - 12:29 AM
Posted 19 March 2006 - 02:43 PM
Posted 19 March 2006 - 02:50 PM
place extra dish on top
wait a few days
most will go away
Posted 19 March 2006 - 09:31 PM
Posted 19 March 2006 - 10:36 PM
Posted 19 March 2006 - 10:55 PM
Posted 19 March 2006 - 11:07 PM
Posted 19 March 2006 - 11:47 PM
Just always keep them upside down. Helps keep the agar moist too. slp/fmrc
This is the only way I've experienced no condensation.
Posted 20 March 2006 - 12:23 AM
Posted 04 January 2007 - 03:09 PM
:rasta:I red somewhere that if you expose mycelia to water directly they die... So Im asking, first is there a way to not have the condensation??? and secondly did I just basically annihilate one of my first dishes???
Posted 04 January 2007 - 05:57 PM
Stable temps while incubating help to keep condensation to a minimum..
i believe some peeps turn their plates upside down to keep water off the expanding myc as a last resort...
Posted 04 January 2007 - 09:18 PM
It's also a good vehicle for getting contams in dishes.
I've had bad luck at pouring at lower temps. In my case the dishes looked great until they went into the fridge after cooling completely. After being put in, they pretty much gave up half their moisture to condensation. Now the agar looks all granular. Which sucks, because pouring more plates sucks.
The best luck I have is to pour hot and stack your dishes 15 or so high while pouring. The added heat to the lid form the bottom of the new dish will keep the condensation from forming and also help push some of the moisture laden air out of the dish. Let them cool completely in the stack. After cooling, use the para film, which is porous to allow for gas exchange.
If you stick them in the fridge they always seem to condense, but with the hot pour and stack, once they warm back up, the condensation should be minimal.
In my experience at least...
Posted 04 January 2007 - 11:05 PM
Posted 05 January 2007 - 08:56 AM
Posted 14 May 2008 - 10:49 AM
it gets TOO wet in side and the pieces of mushroom tissue dont
grow because its way too wet. I cant open it and pour the water out that will just make it contam.
I have some good PE specimens that NEED to be cloned but i cant do it
with all this excess water!
Posted 14 May 2008 - 11:28 AM
Posted 14 May 2008 - 12:00 PM
stack them all vertically then place the plastic sleeve back over them all. Stacking them vertically keeps the top and bottom surfaces of touching dishes the same temperature, so condensation only forms on the sides, and the top dish of each stack. This amount of condensation is easily absorbed back into the agar over the first week.
It’s best to leave the flowhood running until the petri dishes have cooled, at which time they should be wrapped with parafilm unless you’re going to inoculate them right away. Be sure to wrap all dishes in parafilm as soon as you inoculate. Be sure to pour out any leftover agar right away, and wash the bottle very well. If it becomes solid in your bottle, you’ll be looking for a new one.
Posted 14 May 2008 - 01:20 PM
Posted 14 May 2008 - 01:47 PM