44 replies to this topic

[CoyoteMesc]

:lol:
no, no, no

inject hot agar in the jar on the opposite side of the poly filled syringe.

[Hippie3]

you'd need a very wide guage needle methinks
although i've not tried.
i did once try injecting honey/karo thru needles
and gave that up as unfeasible.

[Hippie3]

afterthought-
you could however pour hot with the lid off
seal the lid
THEN sterilize it all over again.

[CoyoteMesc]

yeah, I think even if one has a needle that is a big enough guage it would still be best to do what you just mentioned.
Just pour the agar in and then sterilize em. Pull em out let em cool, pop your airport in the siliconed holes, one drop of solution and BAM. Clean culture!

[Myc]

I've been pouring agar as you suggested. I pc the agar in an airport style jar, draw it into a 60cc syringe, and "pour" my plates like that. Been using an 18ga. needle.
You have to work the agar while it's pretty hot. As it cools, it does have a tendency to plug the needle.
Looking @ local vet supply for 12ga. or 14ga. needles for this operation.

I like the idea of making small "plates" with airport lids and sterilizing. How long would the pc time be for such tiny batches?

[CoyoteMesc]

I dunno, maybe fill one jar and have the others empty. Then unload the pc and draw out the agar and fill the others one at a time? Airport style for all of em of course.

[Myc]

That's what I've been doing....only with petri dishes and using a glovebox. I thought you guys were suggesting "pre-pouring" 1/2 pt. jars and then pc'ing.
I couldn't figure how long to pc 8-10cc's of agar in one jar.

Probably not feasible. I would imagine you would cook such a small amount of agar.

I'm still liking this idea though. With those short jars Hip posted and an airport lid, one could start a culture on the agar, then inject some sterile water and do tissue transfers that way. The culture never gets exposed so long as you flame the needle. Using those short jars and a long enough needle you could scrape the mycel to make an aqueous supspension.

[CoyoteMesc]

yeah, just talking, but that was implied. I have some extra agar. I may do a few test runs and see. The advantage of having them in the jars is you can drip a drop of slurry in( or ms) then like Hip said , after the culture grows out you can squirt some sterile water in and suck it back up. Like the faht jars in a sense.

[Hippie3]

right, i'd make a batch in a flask, stuff the neck with polyfil to store once cooled. then re-heat as needed to liquify, pour as many jar-petris as you need then pc those 20 [email protected] psi .

[CoyoteMesc]

well no need to test now...lol
20 minutes...gotcha

[Myc]

:lol:It would appear as though great minds think alike. I'm just a slow typist. LOL

[CoyoteMesc]

Funny too, we both went back to edit that in :lol:

[Hippie3]

if no pc then just steam for 40 minutes instead.

[joystik]

how would you sterilize prepoured petris and avoid getting water inside them at the same time?

[Hippie3]

well if you use these jars you'd just screw on the lids.

[joystik]

I dont have the jars. I got Glass petri dishes.

well if you use these jars you'd just screw on the lids.

[Hippie3]

i'd use UV light i think...

[joystik]

Never thought of that. Does UV kill spores too?

i'd use UV light i think...

[Hippie3]

yep
btw
i mean UV germicidal, not a 'blacklight'

[kocos]

Great that you brought this up Hip, i was thinking of constructing a small plywood box with such a germicidal UV light mounted inside to disinfect already poured and cooled dishes. However i was unsure of the "glass penetrating" properties of this light. Would dishes have to be laid side by side, or could they be stacked, would the parafilm on the sides have an effect as in casting a shadow and creating "blind spots" etc. Any insight on this would be great.