
Ball Elite half-pint wide mouth mason jars,Best Jar for Agar & Printing.
#21
Posted 23 February 2008 - 11:53 AM
no, no, no
inject hot agar in the jar on the opposite side of the poly filled syringe.
#22
Posted 23 February 2008 - 11:57 AM
although i've not tried.
i did once try injecting honey/karo thru needles
and gave that up as unfeasible.
#23
Posted 23 February 2008 - 11:57 AM
you could however pour hot with the lid off
seal the lid
THEN sterilize it all over again.
#24
Posted 23 February 2008 - 12:12 PM
Just pour the agar in and then sterilize em. Pull em out let em cool, pop your airport in the siliconed holes, one drop of solution and BAM. Clean culture!
#25
Posted 23 February 2008 - 12:42 PM
You have to work the agar while it's pretty hot. As it cools, it does have a tendency to plug the needle.
Looking @ local vet supply for 12ga. or 14ga. needles for this operation.
I like the idea of making small "plates" with airport lids and sterilizing. How long would the pc time be for such tiny batches?
#26
Posted 23 February 2008 - 12:50 PM
#27
Posted 23 February 2008 - 12:59 PM
I couldn't figure how long to pc 8-10cc's of agar in one jar.
Probably not feasible. I would imagine you would cook such a small amount of agar.
I'm still liking this idea though. With those short jars Hip posted and an airport lid, one could start a culture on the agar, then inject some sterile water and do tissue transfers that way. The culture never gets exposed so long as you flame the needle. Using those short jars and a long enough needle you could scrape the mycel to make an aqueous supspension.
#28
Posted 23 February 2008 - 01:01 PM
#29
Posted 23 February 2008 - 01:02 PM
#30
Posted 23 February 2008 - 01:05 PM
20 minutes...gotcha
#31
Posted 23 February 2008 - 01:09 PM
#32
Posted 23 February 2008 - 01:12 PM
#33
Posted 23 February 2008 - 01:16 PM
#34
Posted 25 February 2008 - 10:42 AM
#35
Posted 25 February 2008 - 10:55 AM
#36
Posted 25 February 2008 - 02:46 PM
well if you use these jars you'd just screw on the lids.
#37
Posted 25 February 2008 - 06:02 PM
#38
Posted 26 February 2008 - 10:05 AM
i'd use UV light i think...
#39
Posted 26 February 2008 - 10:06 AM
btw
i mean UV germicidal, not a 'blacklight'
#40
Posted 26 February 2008 - 10:52 AM