This basically is the methods i use with adaptivities to it. It incorporates 9er tek cloning method, Slurry in a Hurry Tek, and some simple things to preserve your clone material for later use in a small fridge.
as many 10 cc syringes or 60cc syringes as you want clone material
room size hepa with ionizer built in
large lawn bags
2 exacto knifes
1/2 pint jar
1 quart jar
combination small safe that fits in your fridge
1) First of all, I start this entire cloning process using a shmuvbox as my glovebox.
shmuvbox. all sterile work is done in here.
behold the shmuvbox. best invention of the century! credit to molester. its very portable and you can get a larger liner that will allow more work space. right now you can see 20 quart jars WBS and two BRF jars in this pic. if you zoom in you can see the two hand holes and the eye hole. the more transparent the bag the better. you won't need the eye hole if the bag is transparent.
this is such a great invention. i sold my flowhood . i have poured plates, taken clones, made spore syringes, g2g transfers, you name it, it has been done in this thing. portable, effective, cheap! can't go wrong!
i use a shmuvbox. it is a HEPA with a big clear trash bag over it, you place all your clean tools, fruits for cloning , and benzo torch to sterilize your blades in this bag.
usually i will wipe down every single part of this HEPA with 70-90% rubbing alc. be anal, spray it with disinfectant spray. this thing will pour plates and take clones easily. holmes brand HEPA filters with a small Ionizer built in are a great size. you only need a small room size filter. and it should be around 30$ at walmart.
once you put the bag around the filter, tape it down to the table your working on. this will in turn, allow the bag to stay put.
now blow your bag up that has all your cooled utensils and sterile equipment in it.
IMPORTANT: PUT YOUR HEPA ON THE LOWEST SETTING. YOU WANT SUBTLE AIRFLOW NOT A DRAFT
turn on the HEPA and when it blows up, cut two holes in it for your hands. one for each. and if you can't find 100% clear lawn bags for the bag, you can cut eye holes as well. this produces positive air pressure and forces out the bad stuff. its real simple, clones can be taken and it stores nicely. its efficient and takes up little space, and hides easily.
2)Follow the 9er tek in the archives
The only thing different I do is pc two exacto blades for quick use. Pced for 20 mins at 15psi. Allowed to cool for 30=45 minutes. i use two sterile scalpals or exacto knives. exactos are easier to replace the blades and you don't have to reorder from online mushroom supply places.
i use one to cut the stem open, the other to cut the tissue out and place in the blender. reason being, i sterilize these with a bernzomatic torch. the bernzo torch has a stand so it stands up. it also has a switch that keeps it going the entire time like a flame lamp only this can melt metal.
once the blades are sterile, i cut the stem open. then use the other sterile exacto to cut the inner stem. exactly like 9er tek but in between each flame sterilization, an alcohol swab is used to cool down the blade.
you can do this in about 30 mins time with everything and cleanup.
i get 1 10cc syringe or even as many as you want to store. i suck up the blended solution into the syringe immediately, rub an alcohol swab on the outside of the vacutainer, then squirt it into a vacutainer. i can sit there and make that solution of clone material into 5-6 viles of vacutainers or even more depending on your volume of water and you stem tissue. one half of a half pint jar filled with distilled water is plenty of clone material. enough for a year if you do this correctly.
this is enough for clones for an entire year for me. i store them in the fridge. also distilled water is used for the syringes. you can store them directly in the syringe if that is all you have. i simply just use the metal blade assembly as my temporary lid when im cloning. i lift it up and suck up with the syringe. then place the lid sitting back on top of the jar with solution. not tight just sitting on there.
this is almost open air, except it is in the shmuvbox. otherwise, there is no small hole in the lid. its just ghetto. you lift the lid, then suck up water in the syringe. out of 100 syringes...0 contams so its working well. even though a bit ghetto.
then you can fill up as many syringes as you want. it may be anal but i always place black electrical tape around my syringes where the lid meets the base. its just an ocd thing i guess.
x) Keeping Cultures: Here is a summation of what I do to keep cultures. And it takes up like a small lock box in your fridge that you can keep locked at all times.
these will store for 2 years at the least if its clean. i always do a 1 syringe test run in two 1/2pint pf jars and let them grow in 2 days.
This is where the speed comes into play. Lets look at it this way, if you have your master sryinges stored in the fridge. All you have to do is inject one syringe into 2 1/2 pint jars. This will grow in a few days with a clone. You then combine this with Tv Casualty's slurry in a hurry tek and you have a clone in a week on grain ready for spawning.
Add Tv Casualty's Slurry Tek and.....well there ain't no and. its just fast!
bernzomatic torch home depot 25$. best 25$ you will EVER spend! guarenteed..
what this does is preserves your clone. you go back to your original syringes everytime. but if your doing slurry in a hurry to grain. well, then you can use one colonized quart to knock up 12 more quarts for a 2nd generation grain to grain. id stick with 2 generations tops. i had trouble with 3 on a clone with slurry tek.
this way, if you make lets say 10-10cc syringes. you get enough clone material for a year or so. i can drag one syringe out for months. its quite fast!
the beauty of it, is that it takes up no space for storage in the back of your fridge. also, its very quick and simple. you can make one half pint of mycelia turn into 120 jars in a few days using tvs slurry tek along with this. fastest way to clone! highly suggested!
Edited by eatyualive, 14 October 2009 - 07:21 PM.