HELP! Melted syringes in PC!
Posted 31 May 2005 - 08:06 PM
I knew that the syringes were for printer use, but I heard you could sterilize them in a PC (brand new PC from walmart $40 down the drain) . Well I put all 10 of them in there and filled it up about 1 inch with water. I then put the rocker thing on the top and left it until it did a gradual rocking. I came back an hour and a half later. I smelt a really funny smell and I thought they might be burned. Well I immediately put cold water on it to release the pressure lock and opened it up. The plastic had melted and dried all over the bottom. I took a knife to it and its hard as a rock and doesnt look like it'll be coming off anytime soon. This is really dissappointing, considering I'm almost broke and $40 is a lot of money.
Now the thing is, that also means I have no syringes. Sort of. You see, I happen to also have a lot of 10cc syringes with a needle about a cm. long. I guess this is far too short. I also forgot to put in one printer syringe with full needle into the PC. I was playing around with it and sucked in some air with it, so it's probally dirty now. Anyway I can use it? Maybe just the needle? BTW, There are no supply stores near where I live so I guess I'm out of luck.
And the worst part is I'm going on vacation. I had been planning to innoculate so that they would be fully colonized for when I got back two weeks later. I only have prints, and prints were very difficult to acquire. If these prints dry out, i'm pretty much screwed. They are on foil, and prtty fresh, so I don't know about their life expectancy.
Well, anyway I can salvage my project? Today is tuesday and I leave for my vacation on saturday. Please help. Thanks
1. Melted syringes in PC
2. brand new PC messed up with plastic all over bottom
3. No syringes now except for 1 unsterile
4. Worried about prints on foil turning bad
5. Project salvageable?
EDIT: I forgot to put in 1 syringe with full needle, but I sucked in air already so its probally dirty, can I just use the needle or is that dirty too?
Posted 31 May 2005 - 08:18 PM
For the record, syringes don't harbor endospores so there is no need to put them in the PC, contrary to lots of advice otherwise. As far as this batch of syringes goes, and your PC, I hate to say you're screwed. If you can clean out the PC, fine, but bear in mind it's only $40 instead of $200 or more for an All American, so it was a cheap lesson. When I use syringes, I drop them into rapidly boiling water for a few minutes, then scoop them out with a fork. I then draw the rapidly boiling water into the syringe and squirt it out several times. As a final cleaning, I draw boiling water into the syringe until it's full, then leave it there. When I'm ready to use the syringe, I squirt all the water out on the floor(mrs rabbit screams when I do this, but I kinda like it when she screams), then flame sterilize the tip before drawing in my sterile water to mix with the spores. This procedure keeps the syringe from melting.
If you use medical syringes, they can withstand autoclaving(PC).
Posted 31 May 2005 - 08:48 PM
PCs are no required and 1 10cc syringe can do as many jars as you want 10 at a time, you just have to keep repeating the cleaning steps rodger suggested and use the one syringe over and over.
PCs aren't required for pf jars.
Posted 31 May 2005 - 09:14 PM
Posted 31 May 2005 - 10:18 PM
Put some water in your pc and bring it to a boil without the top on. Try scraping the plastic off. If that doesn't work I don't know what else to do but I'd be trying something cause I just can't afford to throw away 40 bucks. Good Luck!
Posted 31 May 2005 - 10:32 PM
Posted 31 May 2005 - 11:43 PM
Anyway so, I tried rodger's boiling tek for sterilizing my single syringe and I'm just gonna try and work with that.
One thing though; why would I have to resterilize it after innoculating 10 jars? I realize I would run out of suspension by then, but couldn't I just reflame the needle and I just fill it back up? I guess it wouldn't be too much of a hassle to resterilize it all.
Posted 01 June 2005 - 05:49 AM
Posted 01 June 2005 - 05:50 AM
Posted 01 June 2005 - 06:05 AM
i just wrap my empty syringes in tinfoil and toss them in the
pc when i do jars/bags.
never had any melt.
Posted 01 June 2005 - 10:09 AM
You still have one syringe, clean it and use it, shouldn't be a problem. Exposing things to the air we live in and breathe everyday DOES NOT mean instant contamination! And I don't know what your worry is about your prints on foil. The edges are folded over, right? Should be fine....
Posted 01 June 2005 - 10:31 AM
Posted 01 June 2005 - 10:46 AM
Posted 01 June 2005 - 10:50 AM
Posted 01 June 2005 - 11:03 AM
Danger there is.
Posted 01 June 2005 - 11:47 AM
Posted 01 June 2005 - 08:48 PM
I'm going to go ahead and do some jars anyway with my single syringe. I REALLY hope they don't come out contaminated. Thanks everyone for all your input, really appreciated.
Posted 01 June 2005 - 08:53 PM
maybe my pc is super quality =]