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Rhizo's Lab (Azur, Baeo, Cyan, Friscosa, Ovo...)


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#1 Rhizoo

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Posted 19 January 2008 - 10:43 PM

Hi,

after I started with already colonized woodchips last year - see Woodlover-Regeneration-TEK: Densification of Rhizomorphs by Layering - I got some species as spores respective agar-plates, so finally I had to do sterile work... ;)

Since I'm just following well-known techniques at the moment and I don't have much time either, I'll probably just use this thread to present some nice photos and not so much for a complete documentation of the grows.

At first I used prefabricated agar plates with "Kimmig"-agar:



Ps. azurescens, shortly after inocculation

http://mycotopia.net...=1&d=1200799091



Ps. azurescens, some days later (all 3 pics following were made at the same age with the same scale)

http://mycotopia.net...=1&d=1200799091



Ps. baeocystis

http://mycotopia.net...=1&d=1200799091



Ps. cyanofriscosa

http://mycotopia.net/forums/attachment.php?attachmentid=74136&stc=1&d=1200799091



I also put some spores directly onto popcorn. At first I didn't even have a PC, so I used this TEK http://mycotopia.net...rn-success.html which worked rather well.

Ps. cyanofriscosa

http://mycotopia.net...=1&d=1200799091




http://mycotopia.net...=1&d=1200799091




http://mycotopia.net...=1&d=1200799091




I also got Ps. ovoideocystidata on agar and sucked a little myclium from it in a syringe with sterile water to inocculate popcorn. Unfortunately I got a contam afterwards on the not overgrown parts of petri so I decided to cut out some pices of agar and transfer it to be on the safe side.

http://mycotopia.net...=1&d=1200799885


I dropped the pieces into H2O2 and afterwards on new petris. Since the pieces were so wet I thought I had to move them around in the petri to get rid of the excess H2O2... and got new ovo colonies in their trail:

http://mycotopia.net...=1&d=1200799885


http://mycotopia.net...=1&d=1200799885



The liquid mycelium mixture workerd very well :love:

http://mycotopia.net...=1&d=1200799885




On top of one of the friscosa glass jars I put a layer of sterilized wood. The close-ups show the mycelium growing on the inside wall of the glass:

http://mycotopia.net...=1&d=1200799885




http://mycotopia.net...=1&d=1200799885




http://mycotopia.net...=1&d=1200799885




http://mycotopia.net...=1&d=1200799885

Attached Thumbnails

  • rhizo_cfriscosa_popcorn_4d.jpg
  • rhizo_cfriscosa_popcorn_4b.jpg
  • rhizo_cfriscosa_popcorn_4c.jpg
  • rhizo_cfriscosa_popcorn_4a.jpg
  • rhizo_ovoid_popcorn_1.jpg
  • rhizo_ovoid_petri_2b.jpg
  • rhizo_ovoid_petri_2a.jpg
  • rhizo_ovoid_petri5.jpg
  • rhizo_cfriscosa_popcorn_2c.jpg
  • rhizo_cfriscosa_popcorn_2b.jpg
  • rhizo_cfriscosa_popcorn_2a.jpg
  • rhizo_cfriscosa_petri1_1.jpg
  • rhizo_azur_petri1_1.jpg
  • rhizo_baeo_petri1_1.jpg
  • rhizo_azur_petri3_1.jpg

  • Sidestreet, Zozo, Frequency and 2 others like this

#2 fedshtkpndrk

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Posted 19 January 2008 - 11:19 PM

Posted Image

Pic of the month nominee :eusa_clap That is gorgeous.

-feds

#3 warriorsoul

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Posted 19 January 2008 - 11:50 PM

Great thread!:eusa_clap

#4 Sunstar

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Posted 20 January 2008 - 12:25 AM

Excellent pictures.:kewl:
A question on the jar of frisco you had put the sterilized wood on. Do you hope to fruit directly in the jar?

#5 LocoH

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Posted 20 January 2008 - 04:46 AM

Second that nomination.

Hey Rhizoo, what kind of camera/lens are you using. I have a new rather nice camera with a good large lens. It's 12MP, 20x optical zoom with some kickass image stability software built in. I can see a mole on Enrique Eglises from 3 states away. But by no means could I provide such clarity of a picture through 1/4" think glass that is both concave and moist.

:eusa_clap

#6 Rhizoo

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Posted 20 January 2008 - 08:16 AM

Thanks everyone :)

A question on the jar of frisco you had put the sterilized wood on. Do you hope to fruit directly in the jar?


I havent decided yet. It's tempting, but I'm using this basically for propagation. The popcorn layer below is multi-spore, and by the woodchip layers then a selection towards a fast colonizing substrain happens which I will extract from the top.

But by no means could I provide such clarity of a picture through 1/4" think glass that is both concave and moist.


In the Regeneration-TEK I already described my workflow a little bit. For these pictures I used a superzoom like yours in the maximum tele-position together with a close-up lens. For the last four pics I used a newly bought close-up lens which is achromatic (Raynox DCR-250) and gives much clearer pics than my first (and very cheap) one.

The last close-ups have a width of around 1cm. The trick behind a close-up lens or a real macro lens is that you can place the camera further away than with the usual "macro" switch (which is just changing the AF range) so the geometry is not that distorted by the concave surface. And of course you have to use he largest aperture number available to get the maximum depth of sharpness. But even then if you look closely you can see the the borders are not that sharp as the middle part. Furthermore the pics only work because the hyphae are growing directly on the glass wall, so moisture is not a problem. Further inside the jar there is very nice growth but it cannot be photographed that well, also because the glass walls have a varying thickness which blurs the view.

#7 eatyualive

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Posted 20 January 2008 - 11:13 AM

wow, incredible pics, that camera is awesome!

#8 kocos

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Posted 21 January 2008 - 03:32 AM

Yeah man, your skills with that cam are beyond words. Amazing macro photography.

#9 hyphaenation

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Posted 21 January 2008 - 04:04 PM

Thanks for the excellent closeups. I think these and your other photos will help many.

#10 bear

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Posted 21 January 2008 - 04:12 PM

Beautiful photography...and such clean cultures :)

#11 the_chosen_one

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Posted 21 January 2008 - 06:39 PM

breath taking! :heart:

>>> ARCHIVE MATERIAL <<<



i am particularly interested in the work with the baeo :D
where did you acquire the spores?

#12 MrMycelium

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Posted 21 January 2008 - 06:58 PM

Here we go again, I loved your last thread. Pix are awesome as always. :bow:

#13 joystik

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Posted 23 January 2008 - 11:33 AM

My god!! I got myself a nikon d80 kit with a 18-200mm nikkor lens. I cant take a pic like those you did. Damn, i gotta learn some more.

Awsome pics.
And awsome cultures.


JoY.

#14 Rhizoo

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Posted 23 January 2008 - 02:54 PM

i am particularly interested in the work with the baeo :D
where did you acquire the spores?


Oh, these are from the FSRE - seems they still have some :D. I hope they are identified correctly; we'll see in autumn ;)

My god!! I got myself a nikon d80 kit with a 18-200mm nikkor lens. I cant take a pic like those you did. Damn, i gotta learn some more.


Yeah, when I got my camera a year ago I was not able to take such pics as I do now. And even now I can find something in them which could be done better ;) But now I'm often reaching the limitations of my camera - most of you have much better equipment than me in your hands, so at least you have much more room for improvement :lol:

@kocos: I'm doing these macros of petris the following way:
I use a simple cardboard box whit a hole cut into the top (and a opening at a side to access everything). The lens goes through the hole, the petri is placed underneath (use some books etc. to adjust the distance). A cheap halogen desk lamp is also placed into the box to illuminate the petri (do not forget to adjust the white balance of the camera, or even better use the custom setting). The camera is set to maximum aperture number, lowest ISO number, maximum tele (together with the close-up lens this gives the highest magnification (you could also try a retro-adapter with a normal lens, but never tried this since I don't have a SLR)). And of course I use the self-timer.

The next pictures were also made this way:



Ps. cyanescens "Woodworker", growing from a woodchip
(WW isolated this strain from a wild specimen in europe. In his threads this isolate has shown very nice indoor fruiting properties :) )

http://mycotopia.net...=1&d=1201116371





http://mycotopia.net...=1&d=1201116371


Attached Thumbnails

  • rhizo_cyanww_petri_1a.jpg
  • rhizo_cyanww_petri_1b.jpg


#15 greenie

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Posted 23 January 2008 - 03:17 PM

Got a question for you mr rhizoo: if it was gonna be someones first time growin woodlovers, and they had syringes, what would you reccomend that they do with them? ie inject wbs bags, agar etc?

#16 little white rabbit

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Posted 23 January 2008 - 03:26 PM

nice work rhizoo. your outdoor grows really are great :)

and your pics are even better :bow:



can't wait for fall to see the results of this grow ;)

#17 the_chosen_one

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Posted 23 January 2008 - 03:40 PM

Oh, these are from the FSRE - seems they still have some :D. I hope they are identified correctly; we'll see in autumn ;)


thanks! :) yeah FSRE is usually pretty good. i used to go through the OMS.. the mushroom people/bob harris and company, but they have been steering away from selling psilo-spores for the past few years. too bad, they are also the only source i have found for p. strictipes.. a var i would really love to play with!

many good vibes for you and your autumn beds :meditate:

#18 drtask

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Posted 23 January 2008 - 03:43 PM

i'm simply in awe.... those pics are phenomenal dude. nice work!
i cant wait to see how these all turn out!
keep us posted. peace :rasta:

#19 Rhizoo

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Posted 30 January 2008 - 03:22 PM

Got a question for you mr rhizoo: if it was gonna be someones first time growin woodlovers, and they had syringes, what would you reccomend that they do with them? ie inject wbs bags, agar etc?


Since I'm a beginner with sterile work myself, I can't really recommend a technique. But it is always good to minimize risk by doing it several different ways at once ;)




Friscosa, now with the glass jar filled completely with wood

http://mycotopia.net...=1&d=1201720835



http://mycotopia.net...=1&d=1201720835

Attached Thumbnails

  • rhizo_friscosa_corn2chp_5a.jpg
  • rhizo_friscosa_corn2chp_5b.jpg


#20 eatyualive

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Posted 30 January 2008 - 05:33 PM

woah its snowing mycelia over there.:headbang:




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