114 replies to this topic

[bluehelix]

Description
Grow log of some Panaeolus Cyanescens Cambodian trays plus one novelty container. Based on prior grows, projected harvest is day 26.

Day-by-day Log
Day 1 - Created Panaeolus Cyanescens Cambodian 20ml spore solution in glovebox using single print. Inoculated 600ml liquid culture (LC) with 1ml.
LC is made of, per weight, 2% dextrose, 2% light malt, 96% water.

Day 9 - LC very dense and ready (see pic). This is day 8 after LC started. Innoculated fruiting mix which was 3 large spawn bags half full.

Standard Fruiting Formula

• Manure 75 oz
• WBS 18.8 oz
• water 188 oz
• vermiculite 2.5 liter

Final moisture content confirmed with microwave sample dehydration at ~65% +/- 1% (ideal) Spawn bags were pressure cooked for a full 4 hours for sterilization (normal time).

Conditions for substrate run: 75F with bags spaced about 3" apart on wire racks to prevent over heating.

Day 12 (morning) - Laid trays about 1" to 1.5" deep. Put trays in without any casing or plastic wrap. Circulation is off, except when humidifier switches on. Conditions set to 84F @ 100%

Day 12 (12-hours later during evening) - Trays were laid about 12 hours ago and have filmed over with white mycelium already (see pic). Put 1/4" to 1/2" casing soil on. Casing was at saturation and carefully applied and broken up with a fork for perfect cover consistency and permeability.

Standard Casing Formula (since parts are per volume, these are approximations)

• 1 part vermiculite
• 1 part peat
• 1 part coco coir
• 1/15th part (approximate) aragonite crushed coral
• Enough powdered calcium carbonate and a tiny bit of calcium hydroxide to bring Ph to 7.2

Casing pasteurized at 160F.

Chamber conditions for casing run: 80F with fog-like conditions (ultrasonic mistings most hours)

Day 14 - Casing ~70% colonized

Day 15 - Casing ~100% colonized (see pic)

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[Hippie3]

hi 'helix-
nice to see you again.
:bow:

[eatyualive]

looking good bh good luck.

[shroom_seeker]

i love this pic..

Good luck with this grow!

[Shroomette]

Bluehelix that is such a great grow log......I got some good ideas from it......very nice!!! thanks for sharing. it's awesome:eusa_clap:eusa_clap:eusa_clap

[bluehelix]

shroom_seeker, if you liked that, then check the attachment here out!

Hippie3, hello! Great to see you are still around and doing well!

Thanks for the good wishes from all of you. I have a good feeling about this run. I should have pins in 7 days if things go as I expect they will. This is my first cambodian grow, so I'm excited!

Attached Files

•  lc_day8.avi   1.69MB   635 downloads

[bluehelix]

Today is day 6 after casing and I see massive knotting and already a hundred pins or so. I have never had Pan Cyan trays fruit this rapidly after casing! If the knotting is any indication, I expect thousands of fruits, if not in the first flush, then in the second one. I'll take a picture once things are a little further along, but for now I'll just describe the knotting as if someone took a salt shaker and sprinkled salt all over the trays. The trays with less overlay show the knotting better since they are clearly visible, but in either case, if you look you can see hundreds if not thousands of knots.

Periodic ultrasonic misting was halted and the relative humidity dropped to 97%. I generally don't go much below this in my chamber because the very high internal circulation promotes enough evaporation for the pins to develop normally even at these high relative humidities which would be a problem with less circulation. Plus if I do drop it to 93% or so, the casings will dry out due to the usually high internal circulation.

[eatyualive]

very nice. ive not had cyans do that either. pan. cambodginiensis is the only one besides tropicalis that had that super speed for me at pinning.

nice log. can't wait to see the pics..

[fgl]

Today is day 6 after casing and I see massive knotting and already a hundred pins or so. I have never had Pan Cyan trays fruit this rapidly after casing! If the knotting is any indication, I expect thousands of fruits, if not in the first flush, then in the second one. I'll take a picture once things are a little further along, but for now I'll just describe the knotting as if someone took a salt shaker and sprinkled salt all over the trays. The trays with less overlay show the knotting better since they are clearly visible, but in either case, if you look you can see hundreds if not thousands of knots.

Periodic ultrasonic misting was halted and the relative humidity dropped to 97%. I generally don't go much below this in my chamber because the very high internal circulation promotes enough evaporation for the pins to develop normally even at these high relative humidities which would be a problem with less circulation. Plus if I do drop it to 93% or so, the casings will dry out due to the usually high internal circulation.

So you think is a good idea to let the casing colonized to almost 100% before moving the trays to fruiting conditions?

[bluehelix]

So you think is a good idea to let the casing colonized to almost 100% before moving the trays to fruiting conditions?

No. These are so fast that I would not recommend having the casing colonize at all before moving to the fruiter. If you look at this log, for example, the casing was mostly colonized in 48 hours in the fruiting chamber, but I did keep the temperature a little higher and the air circulation off during that time. My feeling for these is that they are really, really fast. The best runs of Pan Cyans I've had included speed and fog-like conditions (not just 100% RH but mistings) before the first pin set. Between each pin set, you should soak the casing again with heavy mistings or dunks.

[bluehelix]

Here are the pins on day 7 since the casing. Looks like this first flush is going to be small, like the Goliath grow I did a couple months back. I see all the knots, though, so I am sure the second flush will be the big one. This time I'll toss the trays after the big one because the rest of the flushes combined were no more than 10% of the big one and the second biggest in my Goliath grow (this is mostly how cubensis is for me too).

The fruits look like they are going to be very tall, small, and look a little different color than other strains I've grown. The caps look sort of woody with a very dark margin rather than a more pallid light grey-brown cap with no particular darkening at the margin (Goliath and Viets even had a lighter margin than the rest of the cap at this stage). They look potent to me probably because the dark margin looks almost like a bluing reaction. I guess I'll see!

I brought the humidity down just a click to 96% due to the fact that I still see a lot of condensation on the walls.

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[HUMBLE STUDENT]

Thats a nice cluster there! nice pan grow I have been trying with no sucess is this your first pan grow? anyways nice write up and enjoy your harvest

[golly]

Looks promising Blue...Really like the way that colo'd trays surface turned out - just the right amount of myc and the rough texture..:thumbup:

[bluehelix]

psylence, I am not sure what's normal with these, but they do have reddish brown caps before they get big. The interesting thing for me about the color is the dark margin of the cap. I haven't seen that before.

golly, yeah, it really did well. I really like the casing mix I have finally gone with for these. it would suck for cubensis, but the coco coir encourages the mycelium to overlay which is a good thing for pan cyans.

It does look like this is an early flush of sorts, like what I had with my Goliath first flush. The number of mycelium knots is unbelievable! If only 30% of them turn into pins for the big flush, it'll be a very dense flush coming up. This first flush, though, is sort of shabby as you can see in the pictures. I'm sure something much bigger is coming...

[Foster]

Nicely done there bluehelix!:eusa_clap This is pan cyan cambodia correct, not cambodiginiensis? They are a beautiful specimen. They sure are fast. Gotta love that. I had the same experience with cambo, not sure which one, but spawned and cased at same time, 3 days later pin. Keep updating :rasta:

[bluehelix]

Nicely done there bluehelix!:eusa_clap This is pan cyan cambodia correct, not cambodiginiensis? They are a beautiful specimen. They sure are fast. Gotta love that. I had the same experience with cambo, not sure which one, but spawned and cased at same time, 3 days later pin. Keep updating :rasta:

To be honest I don't know for sure. I am not even sure whom gave me the print because I'd had it for a few years. It was labeled "Pan Cambo" so I assumed that meant Cambodian, not cambodiginiensis. What do you think it is? I have a high powered microscope. How can I tell?

[Foster]

Heh, Id just be guessing, but at least I'd have a 50% chance of being correct. That'd be a good question for Workman. In fact, I believe I've asked him before, but am not sure what thread it was in. If you can get a nice shot of the spores he may be able to tell.:bow:

Mine had almost a green tint at pinning, some a lil orange. You can see the fruits in my avatar. Some turned almost gray/blue at maturity.