
spores>Less = better? Thermal Death ? Hydrating ? [merged]
#21
Posted 09 October 2005 - 04:38 PM
#22
Posted 09 October 2005 - 04:46 PM
#23
Posted 09 October 2005 - 05:46 PM
http://mycotopia.net...read.php?t=3558i dont want to hijack the thread or anything, but i 've heard of people using an ultrasonic to disperse spores. can anyone elucidate this or point me to a thread ?
http://mycotopia.net...read.php?t=3663
http://mycotopia.net...read.php?t=3663
#24
Posted 19 November 2005 - 02:33 AM
I find it almost impossible and a big waste of time and money.
Soaking them in water first doesn't lift the spores off it just makes the paper wet. I also tried scraping the spores off with a toothpic and also with an exacto knife but almost nothing came off and the spores were just pushed into the paper.
Considering how expensive these things are it's VERY annoying. Also, I have prints for azurcens and Mexi A and am afraid that they are going to end up a bust just like the pan cyans.
#25
Posted 19 November 2005 - 04:23 PM
Any help is appreciated.
#26
Guest_lost_onabbey_rd_*
Posted 19 November 2005 - 04:48 PM
then throw that into a jar of LC or put it on agar
LOST
#27
Posted 19 November 2005 - 05:38 PM
With this method she gets 4 pouches from each print, and each pouch she makes to hold 20-60ccs of water (depending on size and darkness of the print).
#28
Posted 19 November 2005 - 05:59 PM
This is a very good method. The paper fibers become nill after dilution. I usually do the same except I use a jar and shake the livin' hell out of it several times.Yea foaf cuts a quarter of the print off and puts it in a pouch, adds water via gallon with airport, seals. She takes the pouch and smooshes it around to get the spores from the paper into the water, lets it sit a bit, kneeds it some more, then she fills syringes from the pouch and uses those.
With this method she gets 4 pouches from each print, and each pouch she makes to hold 20-60ccs of water (depending on size and darkness of the print).
Lost has a great method too!
Either way you can cut off any excess paper that does not contain spores first. Helps a little when making syringes. No point in adding extra clogs or a larger home for possible contamination.
#29
Posted 20 November 2005 - 02:41 AM
thanks very much. This is exactly what I was looking for.
Any other methods are welcome as well.
You guys rock. :bow:
#30
Posted 20 November 2005 - 05:49 AM
#31
Posted 10 December 2005 - 12:09 PM
So, should my foaf just trash the whole project and start over with some new syringes, or is there a possibility that the spores will rehydrate within the jars, and start to grow at some point. Maybe just a little delay before the first sign of growth? Thanks.
#32
Posted 10 December 2005 - 01:33 PM
#33
Posted 10 December 2005 - 02:38 PM
and id go ahead and redo it
#34
Posted 10 December 2005 - 09:53 PM
#35
Guest_freakachino_*
Posted 11 December 2005 - 12:18 AM
Lately, I've been making my syringes, and then using my aquarium pump to vibrate the syringes for a couple days to help hydrate and shake em up a bit.
A bit like Hippies Ultrasonic tek for prints and syringes.
You will probably have to reshoot the jars with the hydrated spores. Never know though, we have all seen how much they love to grow! That will be up to you whether you want to redo or not.
Best of luck!
#36
Posted 11 December 2005 - 12:50 AM
#37
Posted 11 December 2005 - 10:54 AM
#38
Posted 11 December 2005 - 11:32 AM
#39
Posted 13 December 2005 - 11:07 PM
A thin layer, I would assume? How long to grow through? I'm guessing the speed of the mushroom myc would give it a big leg up on getting through.
Do you mind if I ask your thoughts on peroxidated and/or antibiotic agars as the second layer?
#40
Guest_dial8_*
Posted 14 December 2005 - 10:35 AM
If you suspect that some mycelium is contaminated you can make up little tubes of straw inoculate one end and the emerging mycelium on the other end should be pretty damn clean. Take this mycelium and transfer to new substrate.