
invitro, agar, etc.
#1
Posted 09 August 2008 - 09:47 AM
I am following a detailed plan right now. This is just a side project to that.
What I am about to show you is a mycelium culture on a agar substitute in the bottom of a half pint jar. It is a test jar for a LC. The strain is Z from a syringe.
What happened is, I was following my plan and testing my LC(2 strains 2 jars each with 1cc of LC each) on an agar substitute(basically powdered long grain brown rice and water pressure cooked into a little cake.) Then a couple days after I inoculated I left town for a couple weeks. While I was away my husband noticed that the agars were not colonizing with anything. So, he reinoculated one jar each of the two strains. The Z took off and the other strain contaminated. BTW, the other 2 jars eventually colonized with myc.
What we have been doing is opening up the jar a few times a day, keeping the myc hydrated, and exposing it to light to see if it will pin. As far as I can tell the jar has looked about the same as in the pictures as it did about a week ago when I got home.
What is everyones thoughts? I think the myc looks really cool and it would be even cooler if I could have a sample of shrooms before my main project is producing.
#2
Posted 09 August 2008 - 10:04 AM
But ya won't get too much!
#3
Posted 09 August 2008 - 11:10 AM
If you are interested I can keep you updated or grab some pics :P
#4
Posted 09 August 2008 - 01:44 PM
BuckarooBanzai, I was reading up on some of the agar threads and seen some agars that pinned all on there own. I think if I don't decide to try a tissue culture with the other agars I will leave them alone and just observe their progress.
#5
Posted 09 August 2008 - 02:55 PM
But each time it was a plate that contained an isolate of a strain I was working with at the time.
So it was the strongest rhizomorphic myc that fruited.
But as BB stated there's no need to keep opening up the jar, IMO all you're doing is increasing your chances for contams.
Good Luck to you 2
#6
Posted 09 August 2008 - 02:58 PM
#7
Posted 10 August 2008 - 05:24 PM
I have a similar jar going, BRF cooked in a 1/2 pt jar. Transfered some B+ spores, grew for a week or two so untill cake was fully colonised. Dunked it just yesterday and now it is sitting in a jar (4 holes in lid) under lights. Hoping it will pin soon :)
If you are interested I can keep you updated or grab some pics :P
The cake has pinned!! Found 4 nice looking fruits pokin out of the cake :) pictures will come when they grow larger
#8
Posted 10 August 2008 - 05:37 PM
The cake has pinned!! Found 4 nice looking fruits pokin out of the cake :) pictures will come when they grow larger
Sweet, can't wait:eusa_clap
#9
Posted 11 August 2008 - 07:42 AM
#10
Posted 11 August 2008 - 09:16 AM
nice pics cns...
#11
Posted 11 August 2008 - 11:37 AM
#12
Posted 11 August 2008 - 11:52 AM
Examples below.

from this thread:
http://mycotopia.net...ar-pinning.html
#13
Posted 11 August 2008 - 12:05 PM
#14
Posted 11 August 2008 - 12:13 PM
It's a helpful way for me to test the fruiting/pinning type of isolate or multispore mycelium I'm working with.
I don't think there can be 100% guarantee put on the fact that the agar dish is going to pin though.
#15
Posted 11 August 2008 - 01:07 PM
#16
Posted 11 August 2008 - 08:14 PM
#17
Posted 12 August 2008 - 06:00 AM
It's great to be back Freaky :rasta:
I have a lot of plates pin, but it's also a sign of a good strain to let run IMO
But as stated before most of the plates are 3rd or 4th transfer of rhizo myc
#18
Posted 12 August 2008 - 08:20 AM
#19
Posted 12 August 2008 - 10:39 AM
#20
Posted 14 August 2008 - 04:09 PM
It kind of just looks like a shadow in the pic and it's not really much darker in person. There is little bits of powdered grain stuck to the inside sides of the jar making it hard to see. I have never opened this jar since sterilization.
To me it looks like a dark blueish or grayish discoloration of the myc. If you couldn't tell it is right in the middle of the bottom of the jar.
Also, I added a pic of the bottom of the jar. I thought that is might have grown a contam underneath the myc which happened to us before. I only see myc growing through what I guess are pockets of air cause I don't think the myc can grow through this stuff. But where you see the white spots on the bottom is directly under the dark spot.
I have a hard time believing this LC is contaminated, but what is going on?
If it is just bruising how did it get bruised?