
Duke's Rice Technique vs. PF
#1
Posted 02 May 2005 - 06:54 PM
My only worries are that:
1. The moisture content of the pint jar is screwed. It just looks wet, but I guess its no different from the pics in the tutorial.
2. The rice in the PF jar wasn't ground enough to work properly. It still had some small chunks in it because I only used a blender.
I doubt either of these concerns will cause much of a problem.
I will update with progress reports on both of these jars. I am not very experienced, but this will be an invitro grow, so I doubt I can screw up much. I really like the whole grain rice technique, because it's just so easy to prepare the jars. Hopefully this will convince me that it's the way to go.
Take a look at the pics, but I warn you, the camera is nothing special.
Peace.
#2
Posted 02 May 2005 - 07:39 PM
#3
Posted 03 May 2005 - 08:10 AM
Looking forward to your updates :)
Peace...
#4
Posted 03 May 2005 - 12:17 PM
#5
Posted 08 May 2005 - 11:38 AM
Hopefully something promising will show up.
#6
Posted 08 May 2005 - 12:04 PM
just soaking rice, no heat ?
#7
Posted 08 May 2005 - 02:39 PM
#8
Posted 11 May 2005 - 05:57 PM
I wonder if this spore syringe may have been weak, since the PF jar only has mycelium in one of the sites. Hopefully, it will be able to fully colonize.
As for the whole grain rice jar, it looks like the moisture content is correct after all; the mycelium is taking to it very well. I think this will probably colonize faster, because of the better air exchange through the polyfill and the better start it has already. But we'll see.
#9
Posted 12 May 2005 - 09:23 AM
good luck
#10
Posted 14 May 2005 - 03:36 PM
As for the PF jar, growth has started in another spot and is a little bigger than a dime there. It seems healthy.
I may inoculate another one of the whole rice jars I made, because I really want this technique to work for me!
Peace.
#11
Posted 14 May 2005 - 04:54 PM
#12
Posted 16 May 2005 - 04:36 PM
Looks like growth might have picked up again in whole rice jar A, but it's hard to tell. Very possibly may have a contam there (I can't tell if it's green or shadow).
Whole rice jar B is not showing any growth yet, but it's only two days after inoculation.
Also, the light bulb has been replaced with one of proper wattage and they're recieving it 24/7.
#13
Posted 23 May 2005 - 06:07 PM
Things are looking great in all of the jars. The PF jar has exploded in rhizomorphic growth and is almost completely colonized by the healthiest mycelium I've ever seen. The growth in whole rice jar A is apparent again, with rhizomorphs visible. The mycelium is steadily taking over the dried out area (caused by my hot light) and can also be seen far down the edge of the jar, so it is penetrating the rice mass. Growth in whole rice jar B has appeared and looks very healthy.
So, many of my worries are gone and I am looking forward to some pins. I really must say that the Classic strain is outperforming B+ by quite a lot.
Peace.
#14
Posted 30 May 2005 - 01:16 PM
As for the whole rice jars, they're still moving but slowly. Jar A is probably 50% colonized and jar B 30%. I think these are suffering because the substrate is too sticky and the mycelium has trouble penetrating it.
When I harvest the first flush off of the PF jar, I'll include some pics.
#15
Posted 30 May 2005 - 01:29 PM
So it's been a week, time for an update.
Things are looking great in all of the jars. The PF jar has exploded in rhizomorphic growth and is almost completely colonized by the healthiest mycelium I've ever seen. The growth in whole rice jar A is apparent again, with rhizomorphs visible. The mycelium is steadily taking over the dried out area (caused by my hot light) and can also be seen far down the edge of the jar, so it is penetrating the rice mass. Growth in whole rice jar B has appeared and looks very healthy.
So, many of my worries are gone and I am looking forward to some pins. I really must say that the Classic strain is outperforming B+ by quite a lot.
Peace.
Life finds a way. The mycelia really likes to grow if you give it decent conditions. Congrats on success so far. I am a fan of the little cakes (even with a bit chunky flour they do great). :D Looking forward to pix.
#16
Posted 31 May 2005 - 05:35 AM
bummer about yer whole rice jars tho',
wonder if a little gypsum/powdered chalk might help that stickiness ?
I checked the jars yesterday and, lo-and-behold, there are pins! This is in the PF half-pint. About 10-15 small pins are developing, and I can see the darker color of the caps on many. I'm very excited for this jar, and I have plans to clone the biggest fruit into honey water and inocculate the rest of my PF jars with that.
As for the whole rice jars, they're still moving but slowly. Jar A is probably 50% colonized and jar B 30%. I think these are suffering because the substrate is too sticky and the mycelium has trouble penetrating it.
When I harvest the first flush off of the PF jar, I'll include some pics.
#17
Posted 31 May 2005 - 06:54 AM
I checked the jars yesterday and, lo-and-behold, there are pins! This is in the PF half-pint. About 10-15 small pins are developing, and I can see the darker color of the caps on many. I'm very excited for this jar, and I have plans to clone the biggest fruit into honey water and inocculate the rest of my PF jars with that.
As for the whole rice jars, they're still moving but slowly. Jar A is probably 50% colonized and jar B 30%. I think these are suffering because the substrate is too sticky and the mycelium has trouble penetrating it.
When I harvest the first flush off of the PF jar, I'll include some pics.
I'm just guessing, but you cooked the whole grain rice instead of soaking it? I'd bet bacteria has got those whole grain jars from the starches brought out of the cooking process. Ya might wanna try to soak the whole grains instead. Just my .02
Neat thread by the way! :D
#18
Posted 31 May 2005 - 07:08 AM
#19
Posted 31 May 2005 - 06:34 PM
Thanks!Neat thread by the way! :D
As for the whole water content issue, I think the only way to really be sure the rice doesn't have too much water is to cook the rice in water before loading the jars. I think I read someone (Duke?) say that this doesn't give the rice as much water as it can take. However, with the differences between different types and crops of rice, using a predetermined water:rice ratio is bound to fail sometimes. Oh well. I still have hope for these jars. The mycelium is growing although there is even a tiny bit of standing water. I bet they will just take a long-ass time to colonize.
+++
I've included some pics of morels found by a friend, my stealth setup, as well as the PF jar (at least 15-25 pins now) and the whole rice jars. The last pic is of some dandelion beer I'm fermenting right now.
#20
Posted 31 May 2005 - 06:52 PM