Mex.a on agar
Posted 28 February 2005 - 08:54 AM
mea plates were inoculated on the 18.1.05
with a sporeworks spore-syringe.
two (pic1 and pic2) produced sufficient growth for the first set of transfers on the 1.205 (pic3, pic4 and pic5),
and from these, a further set of transfers was made on the 8.2.05 (pic6, pic7 and pic8).
they were left to form sclerotia,
the first of which were noticed on the 23.2.05 (pic9).
currently, 6 out of the 36 plates transferred to are showing signs of slerotia formation (pic10, pic11, pic12, pic13, pic14 and pic15).
including one plate from the first set of transfers, which i had decided to hold on to (pic16).
an interesting part of the sclerotia activity
is what appears to be small globules of liquid sitting on their surface.
is this emanating from within the sclerotia itself
or due to condensation from the heat of development?
good thing/bad thing?
also, is it possible to tell from where the sclerotia are forming
as to whether a plate is composed of a single isolated substrain, e.g. pic10?
Posted 28 February 2005 - 09:09 AM
Posted 28 February 2005 - 06:17 PM
well, so far, my money's on that plate in pic10.
Posted 28 February 2005 - 07:19 PM
Posted 28 February 2005 - 07:57 PM
Let's say one of his dishes has ten times as much sclerotia forming as the rest. That's a good master. Transfer a single piece of the sclerotia to a new petri dish. Allow it to grow out for a week or so until the dish is half covered with mycelium. Wrap the dish in several layers of newspaper for insulation against temperature swings, and place in refrigerator.
The original dish with all the sclerotia can be cut 8 ways and each wedge is enough for a quart jar of rye grain. For faster colonization, use one petri dish for 4 quarts of rye. Mex a does very well in grain to grain transfers, so each of those 8 jars can be expanded into ten more to give you 80. Each of those can be expanded into ten more to give you 800 quart jars within six weeks, from a single petri dish.
Posted 28 February 2005 - 08:15 PM
if your going to use a dish for four qts am i correct in assuming to cut into smaller pieces as opposed to one big chunk per jar. creating more innoc points and faster colonization. (^^^^^intrigued by sclerotia and agar work)
Posted 10 May 2005 - 07:29 AM
with a little update on my grow:
6 x 600ml jars of coffee-soaked rye grain
were innoculated on the 4 march
with agar wedges taken from the strongest sclerotia producer (pic10).
600ml jars were chosen over quarts,
shown here side-by-side for comparison,
due to their increased surface area-to-volume ratio,
as observations have been made previously
that sclerotia tend to form more against the glass interface.
unfortunately, these jars have been a wash out
due to the myc refusing to grow off of the wedges.
they were shaken after a week or two,
which helped spread innoculation points,
and eventually fully colonised
but even after 2 months from initial point of innoculation
there has been no visible sign of sclerotia,
apart from this single fella!
in contrast, i also decided to do a batch of 12 x 1/2pts,
syringe-injected with the same isolated substrain used in the 600ml jars,
which had been grown out in a dex jar from a cut of agar.
after only 28 days from point of innoculation
sclerotia growth is evident,
although not consistent across all the jars.
here's a comparison of the three strongest jars so far:
growth on day 28 for jar 1, jar 2 and jar 3
growth on day 34 for jar 1, jar 2 and jar 3
Posted 10 May 2005 - 10:51 AM
Posted 11 May 2005 - 07:35 PM
Posted 12 June 2005 - 07:24 AM
isolated substrains which can be seen at the beginning of this thread,
which i've been waiting on
to see if they'll fruit invitro.
well, here are a couple of solitary fellas i found today.
any suggestions as to what these plates would be good for
Posted 12 June 2005 - 07:49 AM
Posted 12 June 2005 - 07:49 AM
multiply that in liquid nute solution
and then stab some jars ?