
3rd Transfer Texan, Which Looks Best to You?
#1
Posted 08 June 2005 - 07:33 PM
#2
Posted 08 June 2005 - 07:58 PM
looks ropier to me
#3
Posted 08 June 2005 - 08:01 PM
#4
Posted 08 June 2005 - 08:44 PM
#5
Guest_Peter Cottontail_*
Posted 08 June 2005 - 09:20 PM
RR
#6
Posted 08 June 2005 - 09:32 PM
#7
Posted 08 June 2005 - 10:06 PM
#8
Posted 08 June 2005 - 11:07 PM
How would one select the best sector to isolate?
#9
Posted 09 June 2005 - 06:30 AM
I see at least a hundred substrains on each dish. One and three have more substrains than two does, that is why two looks more rhizo. I would suggest using far less spores next time so you can isolate easier. The only real way to tell would be to continue isolating until you have zero sectors. Unfortunately, that would require several hundred petri dishes, then each would have to be grown out. A few of those substrains would definitely kick serious butt. Try picking a few of the sectors and isolate until you have a single sector on a dish. Fruit each single sector isolate to determine the best one, since there is no way you can fruit them all.
RR
perhaps you would define what you mean by
sector
and explain how to identify differences, visual clues
so folks can better understand
why you see hundreds
when they see uniformity ?
#10
Posted 09 June 2005 - 10:10 PM
#11
Guest_Peter Cottontail_*
Posted 09 June 2005 - 11:23 PM
I use an inoculating loop and barely touch it to the sporeprint. When stamets decribed strain isolation in GGMC, he put an inoculating loop worth of spores into a test tube and shook it up. He then dipped the loop back into the water and streaked the spores onto agar. This is much more diluted than a spore syringe and only gives you a few dozen substrains instead of hundreds, making your isolation work much easier. When it comes to spores, less is more.
RR
#12
Posted 10 June 2005 - 12:26 AM
#13
Posted 10 June 2005 - 06:45 AM
archive material
#14
Guest_Peter Cottontail_*
Posted 10 June 2005 - 08:06 AM
RR