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invitro bags: need some guidance


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#21 limeade

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Posted 22 August 2008 - 01:50 PM

those would colonize 5 times faster if spawned to and re sealed, instead of inoculatng with spore or LC, but yes, keep it updated!

Live I use a half cup of spent grounds per block of coir.

#22 crazy1

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Posted 22 August 2008 - 03:39 PM

Didn't have spawn, just an LC, so I figured wtf and went with it.
I do most of my cakes and bags out of LC.
Have good luck with em too.

hey do you add that coffee when mixing for the soak before pasteurizing

#23 limeade

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Posted 22 August 2008 - 06:57 PM

Didn't have spawn, just an LC, so I figured wtf and went with it.
I do most of my cakes and bags out of LC.
Have good luck with em too.

hey do you add that coffee when mixing for the soak before pasteurizing


I add the coffee DURING pasteurization.
You can use cakes as spawn bro, makes great spawn!
I wouldn't do supercakes for spawn as those seem to be used for straight fruiting and your bulk sub will have all that it needs!

#24 BuckarooBanzai

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Posted 23 August 2008 - 12:02 AM

Cakes are fantastically useful in this hobby, even if you never fruit them.

They make excellent spawn - a stand up vegetable grater will reduce a cake to a million points of inoculation in a very short time.

Cakes are also a very cheap and easy way to test an LC or questionable syringe.

#25 limeade

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Posted 23 August 2008 - 12:16 AM

cakes make great gifts for like minded friends, especially done ornately in a fishbowl on a bed of chosen marbles, i like choosing a bowl with a lid. i handed out a few for christmas last year, had it timed to where by new years 1st flush was coming up right on time for fresh doses on new years. The old hippies i gave one two thought it was just the best gift ever, especially since it kept giving, he used the prints from them to start LC's and then grains to do a lot of outdoor work on his farm.

#26 crazy1

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Posted 23 August 2008 - 07:41 AM

yup Buck cakes do make great spawn.
This is the mega cake I shredded up like you said to a bulk sub.

Posted Image

The cheese grater works like a charm

#27 BuckarooBanzai

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Posted 23 August 2008 - 09:05 AM

Gah - I know what you mean. I'm jacking his thread talking about cakes and have yet to mention my own fondness for spawn bags! Hell, I even wrote up a Tek about it.

Back on topic, damnit!

Total apologies Livewire!

BB-Nug Tek - popcorn/coir/castings in spawn bags:
http://mycotopia.net...allon-jars.html

Invitro BB-Nug with her bag pulled off:
Posted Image


And man-o-man do I love those presealable spawn bags. So much less hassle. I don't use the injector port types, though. A blob of silicone with some duct tape over it makes a great inoculation port. Don't forget the duct tape, 'cause the silicone blob will sometimes pop right off the spawn bag without some support.

And don't put the duct tape on before the PC run. Duct tape melts...makes a sticky mess.

#28 limeade

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Posted 23 August 2008 - 09:34 AM

NICE BONZAI, while i know you supplied the link, and i will check it out, i believe i saw those while searching wormcastings, those have wormcastings right?

#29 BuckarooBanzai

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Posted 23 August 2008 - 09:39 AM

Dig it. Castings and coir. I like to add poly, others like verm, some just use the castings/coir. I think the poly makes the best water reservoir - and I hate screwing with the dunk...

#30 limeade

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Posted 23 August 2008 - 09:41 AM

Hah that's a most excellent tek!!

I just read through it!


this is what this thread needed, i simply follow hippie3's repost of hydr0420's method, i like the grains colonizing within the bag though, that's interesting! it eliminates a process for sure!

while we all got off topic a little with cakes, lets re assure anyone that is not wanting to step into grains, but want to go bulkish, that you can continue doing cakes, and use a food grater to grate it up, and use your cakes as spawn! There isn't a need to do any special cake formula either, since it's just being used as spawn, the most basic flour/verm cake can be used.

So cakes do have a little bit o place in this thread, just as spawn though!

and bonzai im just guessing because all things are possible, but have you tried a grain to grain type transfer into those bags? i bet that'd shave a day or two off the time!

#31 BuckarooBanzai

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Posted 23 August 2008 - 10:08 AM

No attempt at a G2G. I don't like to open the bag until I'm doing the first harvest.

I inoculate the popcorn layer with about 90-120mL of hot/fresh growing LC. No need to worry about over wetting, all the coir/castings under the corn soak it up. I always move the needle around a lot, soaking the top layer of corn and getting plenty to drip down the sides of the bag. 60mL syringes are our friends.

The popcorn runs FAST. Usually 4 days at 86F. After mixing, the nugget is usually done in another 5-6 days (depending on volume). I start giving 'em light after day 3 if they are past 80% colonized.

And it takes 'em a little longer, but I don't do any squeezing for fresh air exchange. I love those friggin' filter patches. They are a thing of beauty.

#32 gsmith1981

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Posted 23 August 2008 - 11:15 AM

ya i kinda been checking bucks invitro bags. i just have the roughest time with popcorn. everytime ive tried it the project went green. wish i had better luck at it. even with faht clone jars i have to use wbs.

#33 crazy1

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Posted 31 August 2008 - 09:30 AM

OK here's a grow I"m doing invitro.
The other bags don't seem to moving too fast.
So the other day when I was breaking up the supercake with sand in it I figured I'd start another invitro bag.
This was mixed at about 50/50 spawn to pasteurized compost with 20% or so verm in it.
This was inoculated 5 days ago.

I know it ain't the best pic but you can see it lol

http://mycotopia.net...=1&d=1220195737

Attached Thumbnails

  • invitro1.jpg


#34 gsmith1981

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Posted 31 August 2008 - 10:19 PM

:eusa_claplooking good crazy

#35 crazy1

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Posted 01 September 2008 - 06:18 AM

Thanx Live, I'll keep it updated as things progress.
And since this is being done invitro, it has been given light from day 1.
As I know some have been asking this in other threads.

#36 gsmith1981

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Posted 01 September 2008 - 07:48 PM

maybe thats what i done wrong with pe bag. im gonna try that i just started new tc cause i had so much going on at the moment i never got to finish the thread. in fact didnt finish the grow.

#37 crazy1

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Posted 02 September 2008 - 07:29 AM

Hey Live, I really don't think that not exposing it to light from day 1 would have any adverse effects on the grow.
It just speeds up the time to pin.

#38 crazy1

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Posted 10 September 2008 - 06:23 PM

OK we have LIFT OFF!!!! hahahaha

wOOt ! :headbang:
Here is a pic of the one made with the supercake with sand in the mix.

http://mycotopia.net...stc=1&d=1221091

I know the pic aint the best but enjoy.

Attached Thumbnails

  • 2invitro.jpg


#39 limeade

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Posted 10 September 2008 - 06:59 PM

haahh yeah that looks good!

with sand there would really be no need for a phbuffer i'm thinking.

when i use coffee grinds, (i know this is late) I just toss in a few tablespoons, not much really!

#40 crazy1

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Posted 10 September 2008 - 07:09 PM

thanx lime, I didn't have to adjust the ph at all really.
The compost it was spawned to was neutral as it was aged well.

Man I've NEVER had luck with coffee at all man.
Goes green on me every time i there's coffee in it.




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