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honey tek ??


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#1 Guest_mr._*

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Posted 14 February 2005 - 05:50 PM

I have started my first honey tek. I was just wondering if there is anyway to tell if it is contaminated? Since I have never used/tried the honey tek I don't want to inoculate with contaminated mycelium.

Thanks

#2 Guest_golly_*

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Posted 14 February 2005 - 06:59 PM

Some times It's hard to know for sure until u inoc your grains ..some times they stay semi dormant till given something good to chew on..
Best to try a couple jars before betting the farm--Yup --been burned myself...

#3 elhongo

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Posted 22 April 2005 - 11:04 AM

Hello:

I know this is a "depends on the environment" question, but I have two 1/2 pint jars which are about 6 weeks into puffy growth. They have just been sitting in 70 degree temps for that period of time. I have to go on the road for about 4 months of work. Is there any way, I can slow that growth or even let it sit somewhere for 4 months until I can get into a growing situation?

Thanks

Hongo

#4 Raptor

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Posted 22 April 2005 - 11:12 AM

The closest thing would be to refrigerate it. However, i THINK that living tissue is only good for a month or so refrigerated. Someone else that knows for sure will have to say...

#5 Hippie3

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Posted 22 April 2005 - 11:17 AM

you'll want to cool it to about 40-45 degrees fahrenheit
seal off any air
and light.

#6 Soliver1

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Posted 22 April 2005 - 11:43 AM

4 months is a long time for liquid innoculant,
but if you have no choice then it's the only one you've got.

Let us know how that pans out for you :)

#7 elhongo

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Posted 22 April 2005 - 01:33 PM

Unfortunately, I need to get some new spores outta these honey teks. The spore prints themselves had lasted over two years travelling with me whilst held under incarceration by my overpowering girlfriend. Now, that she is gone, I knocked them out thinking I would probably not get the tek to work, but it worked too good. Now, I have brilliant fuzzy growth(EQ and Hawaii) with no home base. I am thinking that maybe I knock up two sets of neglect jars set for travel with me just before I leave. They could end up in some bizarre places on the road(including air port parking lots), but is worth a shot. I don't think I can travel with the tek jars for much longer(even by car), and may/may not have access to a secure frigerator. I don't trust any of my friends to watch the jars because they have proven to have loose lips. & I don't tell anybody about my hobby.

#8 reefer

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Posted 23 April 2005 - 03:39 PM

I've had great success with honey, and I've kept liquid cultures in the fridge for over 6 months and they worked fine. Just make sure they are sealed well.

#9 green_thumb

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Posted 24 April 2005 - 04:38 PM

i used liquid innoculant (Karo) that had been at room temperature for 1 year for my latest Cambodian adventure. I have petri dishes of South American that have been in the closet for 14 months.

#10 Hippie3

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Posted 24 April 2005 - 04:51 PM

that is incredible.
literally.

#11 maidenofiron

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Posted 09 October 2005 - 03:34 PM

so its been 4 days since i inoculated 2 jars and ever since a teeny bit of mycellial tissue grew on the bottom a few days ago, it has stalled and turned brownish. i am keeping at a little below 80 degrees. also i was wondering if its a problem if the poly-fil from the lid is touching the water? i heard something about the poly-fil getting wet and getting contamed. any one know what might be going on> or is this normal for it to grow slowly??

thanks

#12 Hippie3

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Posted 09 October 2005 - 03:38 PM

the polyfil must be kept dry.
honey will color the mycellia so that's not a big concern itself.
how much honey, how much water ?
how long cooked ?
it's just day 4 so i'd say to see a small cloud or two is about
as good as you can reasonably expect.

#13 maidenofiron

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Posted 11 October 2005 - 04:49 PM

to answer your questions hippie, i put a little less than a teaspoon of honey and a little less than a cup of water, and i cooked it for 30 minutes. id post some pictures but i dont know how too.... help?

#14 Hippie3

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Posted 11 October 2005 - 05:44 PM

use the advanced posting option and scroll down a bit on the page,
you'll see a clickable box titled
manage attachments
click and follow directions to upload from your local computer.

#15 maidenofiron

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Posted 11 October 2005 - 11:53 PM

ok here is what it looks like....

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#16 Hippie3

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Posted 12 October 2005 - 05:09 AM

well, can't tell much,
obviously something's growing in there,
you'll have to test it to determine exactly what...

#17 blackout

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Posted 12 October 2005 - 09:57 AM

It is possible that it is just proteins, they can take a few days to appear depending on the honey.

#18 doodaa man

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Posted 18 April 2006 - 08:41 AM

This is the first time i tried liquid culture.
The honey was knocked up with multi spore PR and the vanilla has pf classic.
Thanks to hip and his thread on basic's of lc in the vaults.
I simmered the jars on the stove. Then knocked up with one cc.
The jars were kept around 85 degrees For two weeks.
I know from other posts and pic's i did not get a lot of growth, But enough to use. I also shot them with one cc of peroxide when i though they were done . Just incase ! IMHO,better safe then sorry !
Now the question.
The mycil in the vanilla has a brown hue to it. Is this normal ? since the vanilla is dark ?
Honey water is clear and the mycil is also.
I know the pic's aren't that great , but does this look ok ? :eusa_pray
I put them in the fridge and plan to see if they will work soon.
Any and all input will be appreciable.

Attached Thumbnails

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  • honey PR.jpg


#19 BuckarooBanzai

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Posted 18 April 2006 - 12:16 PM

Hard to tell from the pics, but FOAF has noticed that the myc in his LCs seems to pick up some pigmentation from the water. The more malt in his LC, the darker the myc will look. It seems to stay pure white and semi translucent in clear LCs, however.

You can always shoot up 2 or 3 test jars to make certain. If every inoculation site contaminates, your LC may be bad.

#20 the_chosen_one

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Posted 18 April 2006 - 02:49 PM

The vanilla can have some sediment in it that will collect in the myc.




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