Posted 10 September 2008 - 03:28 PM
1. sterilize a knife and cut the cap off
2. sterilize a jelly jar with rubbing alcohol (let dry)
3. use plain white typing paper to lay cap on
4. put the jelly jar upside so that it covers the cap and paper
5. wait 24 hours
6. remove jelly jar, remove cap
7. replace jelly jar over spore print
8. let dry for another 24 hours
Posted 10 September 2008 - 03:37 PM
Posted 10 September 2008 - 06:48 PM
It is all easier than you think, man. Just read, read, read and move slow in the glovebox.
Be sure of what you are doing and why, you will be able to do it!
Remember, I had never even heard of cultivating mushrooms indoors 3 years ago. If I can figure this out, most anybody can - I ain't the sharpest spoon in the drawer!
I just don't see how a print could be sterile that comes off of a mushroom that's come off of a cake chillin' in an unsterile environment. How how how.
Posted 11 September 2008 - 09:51 PM
The spores are sterile when they fall, because of the way they grow. Before they fall from the shroom, they are in little sealed sacs, maturing.
As long as you work carefully, in a clean glovebox, very little other than spores will fall on the foil. The spores are sterile when they fall.
Once you have your technique down and have done it a few times, making clean prints is very easy.
Posted 11 September 2008 - 09:55 PM
I'm obviously overthinking. I always do this.
Posted 11 September 2008 - 09:57 PM
The sac is sealed until right before the spore drops, so no matter how dirty the area the mushroom grows, the spore is sterile right up until it drops.
Posted 11 September 2008 - 10:02 PM
Don't know about anybody else, but I've had perfectly usable results just setting the cap on the foil. If you could suspend it, obviously that's cleaner.
I've wondered about suspending in a jar with water in the bottom- maybe they wouldn't stick to the glass that way. Maybe I'll try it some day.
Posted 12 September 2008 - 07:14 AM
Ok ok. I'll be taking some prints soon.