no-heat bleach/lime pasturization
Posted 07 November 2005 - 01:18 AM
After soaking the straw in the bleach water for 24 hours. how long must it sit? Also if it is very damp still.... too wet to spawn... will letting it sit in the sun for a bit be a good idea to remove excess moisture or is there a better way?
Posted 07 November 2005 - 04:54 AM
Posted 07 November 2005 - 06:34 AM
The bag is very compact so I am a little worried about air exchange, bigger bags fom the same manufacturer were looser.
I did stems to get a clone but the stems did have a few spores dropped on them. I am now thinking it may have been better to use a few caps left upside down to drop spores on themselves. I have heard that myc blended too much may not work very well. If I did 4 large caps that would be a shitload of fresh spores in there, so if the live myc didnt work too well the spores should work ok. Plan is to get rapid colonisation before contams get a chance to grow. I could also take unsterile prints on a glass surface and scrape them together, bombard it with about 100 prints worth of spores in one go.
Posted 07 November 2005 - 03:29 PM
Posted 08 November 2005 - 04:59 AM
no signs of growth yet, the bag is nice and humid inside. sitting at about 80F
Posted 10 November 2005 - 12:54 PM
Posted 11 November 2005 - 01:07 PM
I also wonder if there would be any chance of a PF jar working if you just added dry BRF and verm and injected LC as the only moisture for it. May work better with just a shroom slurry due to the lack of sugars.
Posted 13 November 2005 - 08:01 AM
how's the straw bale doing now ?
Posted 14 November 2005 - 07:27 AM
It was removed from the incuabtor, only growth was some seeds that were in with it! My other non-heated poo and lime has also just died on me. It doesnt smell bad but there is no myc now, it has all just gone brown/black.
The straw doesnt smell bad or anything. I am thinking of doing something else with it. Blue Helix was sterilizing bags with manure and WBS. Most say innoculating manure or straw with LC does not work well (I have to agree after my results), but blue helix got good results. I am thinking of mixing the straw with BRF and maybe some manure. This should be able to undergo a simple steaming.
I'm pretty pissed off, but I have plenty of other grows to tide me by.
Posted 14 November 2005 - 08:19 AM
i've found too that mycellial suspensions in liquids
seem to have difficulty in directly making the transition
to straw and other bulk substrates,
i think the shock is enough to give bacteria a head start
Posted 21 January 2006 - 12:59 PM
Can anybody ID these as cubensis? doesnt look like it to me, the caps are perfectly white. I wonder what the chances are that some other spores were on the straw when I bought it.
I will check for blueing.
Posted 22 January 2006 - 11:15 AM
Posted 22 January 2006 - 03:05 PM
The hot tap water works well, but isn't needed if you're soaking for 8+ hours or so. The hotter water knocks off the waxy coating a bit quicker....
You all do know that hot water denatures the active ingredient in bleach, right? It's unclear here, but I might reccomend a hot soak, then cold soak w the bleach, then the lime so the bleach can denature after working it's magic. Mind you I'm new and have never worked with straw but...science says!