Next step after cardboard cloning?
Posted 03 April 2005 - 05:50 PM
And while this newbie is asking questions; is it possible or practical to clone direct to say a grain substrate? Or is it necessary to clone in liquid or agar first before expanding spawn?
Where are flow hoods necessary? for what steps in cultivation? are they necessary for grain expansion to more grain or straw? Would a heavy shower of negative ions do the job equally well?
I put an ionizer in our kitchen and after a few moths it dawned on me that we never had food spoiling in the fridge as we had before. Surely this must be useful for mushroom cultivation.
Posted 03 April 2005 - 06:14 PM
Posted 03 April 2005 - 09:13 PM
When I pick wild mushrooms for cloning, I always take along some moist cardboard. This is much easier and more forgiving in the woods than agar. On a two or three day trip, it's not unusual for the fruits to begin sprouting mycelium into the cardboard. When I get home, some of this fresh mycelium is transferred to agar to give any contaminants present a chance to grow, so I can then transfer away from them. Were you to clone straight to grains or liquid, the contaminants are as likely to grow as the mushroom tissue. Good luck!
Posted 13 April 2005 - 08:03 AM
So these didn't have the same period of H2O2 on them, and are potentially contaminated. I cross my fingers and hope that the reputation of Oysters as being vigorous and aggressive colonizers lets me get away with it.
I placed the transfered bits of cardboard face down as I was thinking this would speed colonisation- is this correct? or are they better left face up in the air?
........Aside: It has occurred to me that since I lack petri dishes, these sterile bags are an adequate substitute, tho a little fiddly.
I took the plastic bag which contained the cultures that had and hadn't been H2O2 dipped, and as best I could scraped away the visible original mushroom tissue. This was to prevent rot/contamination.
It had turrned slushy. I guess this tissue, differentiated as it is as fruit tissue, is not up to staying healthy as spawning tissue.
I added new pieces of H2O2 treated cardboard to the original culutres in jars to give them something to grow onto now that I have cut big bits away.
- Was this the right thing to do? at worst I suppose it may contaminate, or just waste time if it doesn't colonise.
I also cut cardboard from the piece colonised by the piece of mushroom cap
I put one cut off piece directly on fresh coffee grounds/filter paper in a fresh plastic bag, and others on more cardboard in bags.
However, since these pieces grew simply from a slice of cap, whats the chance that they also contain culture of germinated spores and not pure culture? I shall keep everything seperate and isolated.
I put a dozen or so pieces of thick grey cardboard into 3% H2O2, and put them in a plastic bag for later. When I need to start a new culture, I'll just rinse them off with cool boiled water first.
Well thats all so far.
Do mycelia "run out of steam" or something? is it due to a medium not providing sufficient nutrients? This is happening to my wild Oyster culture what is a good all round nutrient to add? I was thinking of beer broth[ 1 part beer, 3 parts water, PCed] or dissolved multi or B vitamin. Can I spray that on the medium? or dunk it?
I have had a few brainwaves that I will keep to myself for now and try out later. If they work you will read it here first.
Please add your comments.
Posted 14 April 2005 - 06:32 AM
since these pieces grew simply from a slice of cap, whats the chance that they also contain culture of germinated spores and not pure culture?
Posted 14 April 2005 - 06:33 AM
Do mycelia "run out of steam" or something? is it due to a medium not providing sufficient nutrients? This is happening to my wild Oyster culture what is a good all round nutrient to add?
no beer unless you boil off the alky
i'd try a dilute malt extract
but may well increase contam risks
Posted 14 April 2005 - 03:59 PM
Posted 05 June 2005 - 08:51 AM
I am still a beginner but gaining experience slowly.
I have for the second time cloned store bought Oysters with a view to growing them.
So far they are only growing as mycelia on cardboard.
How long should the mycelia grow before cutting it away from the original clone sample and growing it on to another medium? The original samples were taken on 30 May, a monday and today its 6 days, a sunday, 5,june.
I used a different cardboard than the first time and it seems not to have grown so fast. I tried water on the cardboard, and MultiB vitamin solution, and a combination.
How should I grow out the samples? put on further cardboard with other nutrients?
I have taken some old muesli- perhaps I should use fresh?- and its a mix of wheat, oats, and barley seeds, rolled, and put it in boiling water and let it cool down . I was going to let it soak for 24 hours then should I PC it?
I also chopped up some straw from a nearby farm. I wont wet it till I am sure I am ready to begin spawning.
I am just not sure what steps to take and when, and I don't want to waste my efforts. The last time I did this the mycelia didn't survive the transfer to more cardboard. I suspect it was due to a lack of nutrient which is why I used MultiB vitamin solution this time.
any hints or directions would be graetfully received.
Posted 05 June 2005 - 10:22 AM
Posted 05 June 2005 - 05:37 PM
I will make it a point to cut from the leading edge. I should cut the leading edge of the cardboard, yes? and then to agar, and then cut the leading edge to a new agar.
I think I noticed from my first attepts that the bigger the initial sample, the faster and further the mycelium grew. Must be coz it had a bigger reserve of nutrients.
I had attempted to transfer the growth directly to Coffee grounds with filter papers but it just didn't happen, and I didn't know why then but as mentioned, now suspect it was lack of nutrients.
I don't have petri dishes. I have jam jars. I can get Agar, Malt/extract and Karo from the local Asian supermarket.
Any particular tek or class of tek i should follow?
Would a liquid culture attempt be too optimistic at this stage?
hope you like the questions 8-) !
I am enjoying adapting and improvising...sure is a challenge
Posted 05 June 2005 - 06:02 PM