tek for syringe making? [merged]
Posted 02 March 2005 - 04:51 PM
I know many make syringes by using a glass, scalpel and syringe. Most will put the water in a glass, scrape the spores into the water, stir it up and then suck up the spores in a syringe. When you do this the spores want to float on top of the water and stick together and stick to the sides of the glass.
If you aren't using jetdry, which would help the spores not stick together, I recommend putting the spores in the glass FIRST.
Scrape the spores into the dry glass. Then you can squirt all your water in on top of the spores. It seems to break them apart much more effectively and distribute them throughout the water more evenly, keeping large chunks from sticking to the sides of the glass.
Posted 02 March 2005 - 05:17 PM
Posted 02 March 2005 - 05:51 PM
set that glass on a source of ultrasound
[like a jewelry cleaner or a humidifier]
and shake those spore clumps apart
Posted 02 March 2005 - 05:54 PM
Posted 02 March 2005 - 05:58 PM
so the sound can conduct better
Posted 02 March 2005 - 06:22 PM
I've tried putting jars of water on top of the transducer elements, but it doesn't seem to agitate the water, so I don't think it's doing much of a job.
Posted 03 March 2005 - 10:26 AM
Another idea - a shot in the dark - lay your syringe on your cat. I recently read that a cats purr causes almost the same vibrations as ultrosonic that is used for pain. You can actually hold a purring cat and it will help reduce pain. Maybe it will break up those spore clumps too! :)
Posted 03 March 2005 - 11:06 AM
just a note, tell your wife or husband that your doing this ahead of time.
Posted 19 October 2005 - 10:07 AM
Posted 19 October 2005 - 11:31 AM
Leave baggy open for air exchange while printing.
When ready to make syringe.
Simply take new syringe , fill with sterile water , inject water into the baggy , mix with your fingers OUTSIDE the baggy , Suck water back into syringe.
I find this ti be the easiest way for me.
Posted 19 October 2005 - 11:38 AM
Posted 19 October 2005 - 12:07 PM
If its a big print and very dark, you could get 10.
It all depends.
Posted 19 October 2005 - 12:38 PM
Posted 19 October 2005 - 12:39 PM
Posted 19 October 2005 - 12:42 PM
Posted 19 October 2005 - 01:07 PM
scissors.. one large.. one small
2 half pint jars
2x3 ziplock bags.. you can get them on ebay for about $5
ISO.. paper towels
large tupperware.. i use a 6qt.. about 6x12
gloves, mask, oust
(optional) shot glass and decedent
ok.. first thing is first.. take your tyvek and large scissors and cut the tyvek in to about 1.5 - 2in squares.. i like to cut about 25 or 30 each time..
now put all of your squares in 1 of the half pint jars.. then put on a double layer of tyvek and the band with foil on top.. no solid lid
then put your small scissors and tweezers in the other half pint jar with the same lid set up
pc those for 20 mins (or whatever) i normally just throw them in with what ever i'm pc'ing at the time.. i know it's a bit extreme but i like to make sure my stuff is sterile
now when it comes time to print..
put on your mask.. so you don't breath bacterial all over everything
gather your stuff on a cleared table
tupperware, foil, 1/2 pint jars from pc, thumbtacks, gloves, oust
wipe off a good working area with ISO..
put on your gloves and clean them with ISO
wipe down the tupperware with ISO.. make sure to get the lid too
then take a piece of foil off the roll and lay it inside the tupperware.. wipe it down as well
also take a minute to wipe down your thumb tacks and set them aside
you want everything good and clean..
now while i go to retrieve the caps i spray a good dose of oust into the room
get your fruiting substrate and set it off to the side a bit
take a quick moment to determine which caps and how many you are going to print.. you can go ahead and put your thumbtacks in the tops of the ones you've chosen
once you got it figured out.. re clean your gloves with ISO
take the lid off both the half pint jars..
take your tweezers and set out a piece of tyvek for each cap you plan on printing.. if they are small caps i put two on one piece of tyvek.. if they are large caps I use 2 pieces of tyvek..
take your small scissors and cut the caps off.. make sure to hold onto the thumb tack while doing this..
now just transfer the caps one by one to their respective tyvek squares
once all the caps are transferred put the lid on the tupperware..
find a good clean dark spot to let it sit for a 12-24 hours
(i put the lid on tight while carrying it, then take it off and leave it slightly ajar once it's sitting where it will stay)
once your caps are done printing
set things up exactly as you did for the first time as most caps can be printed more then one time.. go ahead and lay out the tyvek and put the lid on the tupperware
go get the other tupperware from it's printing spot .. clean your gloves again
right after cleaning your gloves take out your ziplocks and go ahead and crack the seals.. as this will be much harder to do with tweezers in hand later on
now take the lids off of both the tupperwares
then just pick the caps up by they thumbtacks and transfer them to the new tyvek..
take your tweezers and wipe them with ISO.. then flame them with a lighter for a second.. wipe them with ISO again..
now in one hand hold the tweezers .. and in the other hold your ziplock baggie
take the tweezers and use them to open the ziplock.. since you cracked the seal earlier just put them 1/4in inside the bag and move them back and forth along the seal
now pick up your print and place it in the bag
i like to leave the bags cracked open and put them back in the original tupperware with a small amount of decedent for 24 hrs.. but this is entirely optional..
if you chose not to do this go ahead and seal the bags right after you place the prints in them.. if you go the decedent route.. just seal them up with a gloved hand when you remove them after drying..
the reason i adapted this tek was for it's simplicity and ability to do in open air.. it also makes making syringes much quicker and easier, which can also be done in open air with good clean procedure.. which means you can do the same thing with shooting up jars and or myco bags... just be sure to wipe everything down with ISO or bleach and give the air a good shot of oust before starting.. one can actually go through the hole process with no glovebox.. just need a good clean draft free room, gloves, mask, ISO and paper towels..