Im new to agar, and here are a few of the first plates I did.
This first one is just a scraped TX print to PDA.
Im curious as to why Im not seeing very rhizomorphic growth compared to many I see online? Did I just happen to scrape a portion of the print that didnt contain any strong substrains?
I dont really see any sections that look better then the others as far as isolation goes. Im not planning on isolating, I just wanted to try to germinate some spores on agar with a print that I didnt need for practice before I used my good prints. But if I was to continue with the plate ... I dont see anything worth selecting do you?
Below is a piece of GT mycelium that I saved from extinction .. haha. I got a contaminated syringe from a vendor. I love this particular strain from this vendor it has always produced fantastically for me even from MS. Unfortunately with the syringe being badly contaminated everything it touches turns to shit in a hurry. I happend to see a small whisp of mycelium in one of the jars that I grabbed with a tweezers, dunked in h202, then put on this dish after I swished some h202 on the surface of it.
It looks really fluffy and white, but not rhizomorphic. Now you can see just in the last few days it has jumped to the plate (its 15 days in). Im curious, the growth extending from the center piece looks a bit more rhizo but still quite whispy ... is it possible for this piece .. looking like it does to produce rhizo sections as it grows? In your experience? Or did I happen to just get another weak substrain on this round as well? This makes more sense that it could happen in this case since it was a small piece of mycelium I grabbed, but in the tex print situation above .. not so much I would think out of all the substrains that landed on the plate one should have been rhizo (there was a bunch of spores on the plate from the tex print scraping.)
Edited by Sidestreet, 14 August 2015 - 09:45 AM.
Re-posted images in-line