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Hip's invitro tek [merged]


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#1 Guest_magic_*

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Posted 13 February 2005 - 06:27 AM

Would it allow for better growth if som of the Vermiculite layer were let off to make more room for the fruits to fill?


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#2 Guest_rodger_*

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Posted 13 February 2005 - 07:23 AM

It's common for invitro growers to open the jar up after full colonization to dump the verm barrier out. Don't leave it out from the start as that's your filter to protect from contaminants.

#3 Guest_hippie3_*

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Posted 13 February 2005 - 08:56 AM

there really is no need to make more room,
invitro will still form fruits
in a full jar.
i just shake off the loose verm after first harvest

#4 cutty

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Posted 13 February 2005 - 10:39 AM

there really is no need to make more room,
invitro will still form fruits
in a full jar.
i just shake off the loose verm after first harvest


Its amazing how the fruits just force thier way against the glass and make room for themselves.

#5 Lazlo

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Posted 13 February 2005 - 01:59 PM

The cake will shrink a little bit because it's loosing moisture.

#6 Guest_mycopelandia_*

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Posted 15 February 2005 - 10:38 PM

I want to do a bulk invitro grow. Ive read something about popcorn invitro and amazing results, can anyone give me a link to a popcorn invitro tek? I haven't found one that explains it yet.. if there is one. But anyways, what tek is the way to go for best yeilds from invitro?

#7 Lazlo

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Posted 15 February 2005 - 10:45 PM

I would think BRF in 1/2 gallon widemouths for bulk invitro. Damn, i just thought of that! Sounds pretty cool huh! Damn, i might have to give that a shot. I'm just throwin you an idea.Posted Image

(Message edited by looper on February 16, 2005)

#8 Guest_perpetually_*

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Posted 15 February 2005 - 10:48 PM

well ususally grains do fairly poorly invitro from what i've heard.. i'd be interested in this too if anything turns up...
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#9 cutty

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Posted 15 February 2005 - 10:56 PM

I do many invitro grows,and I find that the BRF tek works the best IMO,I have tried grains and popcorn and I find the yields arent as Plentiful.

#10 Lazlo

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Posted 15 February 2005 - 11:02 PM

Whole grains alone don't hold water like the BRF. I just ordered some 1/2 gallon tapered wide mouths and i'm going to give it a shot. With ground up bird seed though.

(Message edited by looper on February 16, 2005)

#11 cutty

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Posted 15 February 2005 - 11:12 PM

<blockquote><hr size=0><!-quote-!><font size=1>quote:</font>

I would think BRF in 1/2 gallon widemouths for bulk invitro. Damn, i just thought of that! Sounds pretty cool huh! Damn, i might have to give that a shot. I'm just throwin you an idea. <!-/quote-!><hr size=0></blockquote>A foaf has 2 (1.5 Litre) Mason jars half way colo'd right now with BRF + Verm.I'll get him to let one go invitro and post pics.These jars do take a while to colo though.It will probably take atleast another month to finish colo.

#12 Lazlo

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Posted 15 February 2005 - 11:27 PM

I would think with karo i can get them colonized in 2 weeks. If the formula works with the WBS. I don't see why it won't. How long did your buddy pc the jars?

#13 cutty

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Posted 15 February 2005 - 11:36 PM

<blockquote><hr size=0><!-quote-!><font size=1>quote:</font>

How long did your buddy pc the jars?<!-/quote-!><hr size=0></blockquote>90 minutes...He nocc'd em up with multispore 10cc in each jar.I know 10cc is alot but hes tryin to get the jars to colo a lil faster.Its already been a month and there about half colo now.

#14 Lazlo

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Posted 15 February 2005 - 11:43 PM

Yeah, with the karo i should be able to coat most of the cake with it, speeding up the colonization considerably. Awesome! Thanks Cutty.

#15 cutty

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Posted 16 February 2005 - 12:23 AM

<blockquote><hr size=0><!-quote-!><font size=1>quote:</font>

Yeah, with the karo i should be able to coat most of the cake with it, speeding up the colonization considerably. Awesome! Thanks Cutty.<!-/quote-!><hr size=0></blockquote>Np bro, Yeah I would agree with ya, Karo or any liquid culture would definetely speed up colo.

#16 Guest_rodger_*

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Posted 16 February 2005 - 03:10 AM

I've had some success with rye berries and invitro growing. If you use quart jars of rye, they'll fully colonize in two weeks, and within a month to six weeks you'll have good results

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#17 Lazlo

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Posted 16 February 2005 - 09:40 AM

Very nice. What strain is that? And how do you get your fruit out of there?

#18 Guest_rodger_*

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Posted 16 February 2005 - 09:57 AM

Just reach in and dig em out with a fork.
PR's on top and BR on the bottom.

#19 Guest_strangeland_*

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Posted 16 February 2005 - 10:23 AM

Curry had some nice results with a substrate of 50/50 millet/verm.

Check out the tek here:
http://mycotopia.net...tml ?1098884276

If you want results like Rodgers jars above,
strain isolation on agar is the way to go.
Isolate a strain that easily fruits on agar and it should do great invitro.



#20 Lazlo

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Posted 16 February 2005 - 10:49 AM

I'll skip the agar. For clones i like karo. I do want to try out Stephen Peele's hot agar tek cleaning up prints.




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