Hip's invitro tek [merged]
Posted 16 February 2005 - 11:13 AM
Posted 16 February 2005 - 11:19 AM
200 full-pint jars
Posted 16 February 2005 - 11:22 AM
at least not without
first isolating a proven substrain.
Posted 16 February 2005 - 11:30 AM
Posted 16 February 2005 - 11:48 AM
I think I remember seeing a pic posted awhile back of a light fixed to the underside of a rubbermaid lid to initiate pinning/fruiting and provide an automated setup since the light was on a timer. I've searched High and Low but have been unable to find that pic... Does anyone use this setup or know of a cheap light supplier. I'm already familiar with tap lights, I'm interested in automation. Thanks!
Posted 16 February 2005 - 11:58 AM
Posted 16 February 2005 - 12:06 PM
Posted 16 February 2005 - 12:13 PM
holy shit-you the man!!
...I guess you just got a lot of really hungry friends hhuh?
Posted 16 February 2005 - 12:29 PM
I think the fastest way would be to spawn with existing brf cakes...I'm thinking if you sterilize a bunch of 500ml jars of brf mix, you could spawn from 250ml jars, ending up with a bunch of 500ml invitros.
What's your method with 200 500ml jars Candykid420? How long have you had that setup running?
Posted 16 February 2005 - 12:33 PM
if you really wanted true bulk invitro
in a reasonable time-frame
my approach would be
brf/verm mix in mycobags
so you can knead/squeeze them to speed up colonization
then once fully colonized
one could cut away bag
and pack colonized substrate into
whatever rigid container one chooses,
even half gallon jars.
Posted 16 February 2005 - 01:22 PM
Posted 16 February 2005 - 01:28 PM
Posted 16 February 2005 - 04:22 PM
And the mushrooms fruit just as well in 500ml jars than in 250ml, if anything you get more out of the 500ml because they have room to grow longer and fatter. I get at LEAST 3 grams dry off of one 500ml (as opposed to 1-2 grams with 250ml), plus it will flush again.
It just takes a LONG time to make and cook 200 jars (but the process becomes much shorter with the help of four pressure cookers).
I know there is a way for better yields (straw logs, casings) but if you want bulk INIVTRO, then my method is the best way. There is only so much one can harvest from a single invitro jar, you might as well make a ton of them if you want bulk.
Posted 17 February 2005 - 10:14 PM
Nice. I once was up around 40 250ml jars, regular PF cakes; some of them I fruited inside 500ml's, but making the cake that size in the first place makes more sense. I had a smaller PC back then, mind you...wasn't possible.
I used standing metal racks with white sheets draped over them, using ambient light from windows. Of course, I dunk between flushes and bleach dip starting after the second flush.
Four pressure cookers! I love it! I'm tempted to ask what your weekly dry harvest is, but that might be the wrong Q to ask here.
How are you inoculating your 500ml brf jars? I would think the quickest method for colonization would be spawning...what works most quickly for you? Is using spores on large jars very slow? If spores, are you using only 4 inoc points, or more?
May I copy your brain? I am playing with popcorn res effect and soon wbs too but invitro is the most reliable method I've done. Plus I don't want to be scheduled into misting, etc. I would like to see what potential your method yields.
I'm curious as to the subtleties of your operation; 1. Do you label the lids of jars to have an idea later when one should be thrown out - you can't keep tabs on if a jar has fruited in the last 2 wks.
Posted 18 February 2005 - 03:24 AM
Thanks for the reply Hippie3, I'll read up on mycobags.
I suppose a working method I already have would be to use colonized 500ml brf jars as spawn with sterilized 500ml jars of substrate, load into 1.5L jars inside a glovebox...probably much more work though!
At the moment I'm trying invitro with res-effected popcorn. I've got a 2-jar popcorn & verm cake upsidedown in a transparent tupperware container with holes in the lid. I also have four coir-popcorn res cakes with some verm on top and underneath in rectangular tupperware w holes in the lids. That's two 500ml jars of popcorn mixed with verm or coir in a 1:1 ratio, with about an inch of airspace above them in the containers, and a quarter-inch on the sides. Think they'll work?
Neglect teks feel time effiecient to me...:)
Posted 18 February 2005 - 08:21 AM
Posted 20 March 2005 - 08:44 PM
Posted 20 March 2005 - 08:45 PM