Hip's invitro tek [merged]
Posted 20 March 2005 - 08:53 PM
Posted 27 March 2005 - 08:44 AM
i'd say you may be on the right track,
just need a better formulation,
maybe a bit less poo ?
Posted 27 March 2005 - 10:05 AM
the first is the same cake different jar, the second I stirred the casing and misted because it got dry, the third seemed moist still so I let it be, and the fourth is the one in the thread pan casing that I have removed most of the casing from and intend to recase.
Posted 27 March 2005 - 04:28 PM
Posted 17 April 2005 - 04:04 PM
I know that 99% of beginners fail because they deviate from established teks, but in my case I don't think any conventional tek will fit my situation (mainly the lack of PC/stove). My plan is to grow invitro (verm layer on top and bottom) on hominy using the microwave to steralize and I want to use the brazilian strain for its fast colonizing which will hopefully aid in fighting off contams (will Brazilian grow invitro? If not will Z's?).
My whole plan basically hinges on the idea of using hominy as an invitro substrate so if thats not possible I guess this a moot post for the most part. However if it is possible, what do you all think of using hominy as a micro-steralization substrate? I think I have read through most of the archives about micro-teks but that is the main area where I am lacking a lot of info, do any of you do it? Have you? Is it naive of me to think I can pull it off?
Posted 17 April 2005 - 04:53 PM
Hominy on the other hand, does not, i got one jar to fruit invitro through abuse, but in general they won't do it.
A casing is VERY easy though, heres what i did:
Get a WIDE jar, like 6 inches wide kind of wide, and at least 6 tall as well, check the doller store.
Crumble (gently!) the grain (hominy, in this case) and try to get it as level as possible on the bottem of the jar, then take your damp verm (dampen it till you can give it a good squeeze and only get a little drip or two of water out them bottem), and spread maybe 1/4-3/8ths of an inch of that over the top of the grain.
Layer some duct tape around the bottem of the jar so that the grain isn't exposed to light.
Now put the lid back on the jar, and put it in a warmish spot with light (65-75 degrees. 70-75 is best), at LEAST twice a day (preferibly three times) take the lid off and use it to fan the old air out of the jar, 10 seconds of fanning is enough.
In a week or two(or three, or four, sometimes), it should start popping little pins up, if it doesn't keep this up.
Should the verm start to dry out a bit, lightly mist it with water, LIGHTLY! Don't mist if you can see little white round thingies (hyphal knots) or tiny tiny mushrooms (pins), as it can kill them.
Because the lid stays on, you don't lose nearly as much water to evaporation as you do with a normal open casing, so you probably won't have to mist till the first flush is getting largish, but your mileage may vary.
Thats what i did my first grow, which happened to be with hominy. I didn't get a nice even flush like the pics you'll see, but i got a bunch of mushrooms from it.
Posted 23 April 2005 - 07:03 PM
i'd intermix the verm [coir even better] 50/50 with hominy
then put the filter/barrier of verm above like a cake.
then steam it an hour, maybe even two.
'wave sterilization is tricky,
you might get a few thru ok
but also expect to lose several.
steam is better.
Posted 23 April 2005 - 08:22 PM
Posted 03 November 2005 - 10:13 PM
Posted 04 November 2005 - 09:25 AM
Posted 04 November 2005 - 09:30 AM
Posted 07 November 2005 - 06:44 PM
I'm going to scrape off the mushrooms inside a glovebox and use birdseed as spawn for a casing. Can't beleve how many and how big some of these got. Look at the bulge on the top of the jar in the first pic. They started to open, invitro, and are pushing at the tyvek!
Posted 08 November 2005 - 12:33 AM
Here's a few Qt jars of wbs, not yet fully colonized, but pinning.
You can achieve good invitro growth from any grain.
Wbs, rye, brf, corn etc.....
Posted 08 November 2005 - 08:08 AM
i've seen more than enough examples to be quite sure that,
at least with multispore inoculations,
one gets better invitro performance from cakes [rez-effect],
so if you did want to pursue
it's good to mix some vermiculite in with the grain.
Posted 08 November 2005 - 11:32 AM
I also agree that cakes are better for invitro grows. They hold up better imho.
Posted 21 November 2005 - 05:55 PM
A FOAF was scraping the sht (wispy colonized verm w/ a little BRF) from the tops of his PF jars and using an extra ziploc bag to hold the waste. When he's done the bag with the small bit of substrate is set aside for disposal when the birthing procedure is finished. After a couple weeks my FOAF realized he forgot to get rid of that waste, but by the time he reached it there were several mushies growing invitro in the ziploc. They had been sitting in a dark area in a basement at 60-70 deg F.
By my estimate, there was maybe 2-3 Tbsp. colonized material which yeilded about 10g wet. That's not bad for a one-time flush.
I'm thinking why not slice up a cake into rounds and drop each in a seperate ziploc after a 200:1 bleach dunk? They should do well with little/no attention. Say 7-8 ziplocs per cake each yeilding 10g wet each. 7+g Dry all in one harvest. Consolidating all the fruiting to one flush would eliminate dunking between flushes, as well as the need to tend to humidity and light. Light would probably speed things up, but wasn't an issue in the accidental "trial" bag. It seems to me that the key to getting the most out of your spawn is to trick the myc into fruiting as fast as possible so any would-be contams never get a foothold.
I'll try and convince my FOAF to try this with one of his cakes that are just coming out of incubation today. Anyone else have ideas/opinions/useful info on the topic of novel invitro methods?
Posted 21 November 2005 - 10:48 PM
Posted 23 November 2005 - 12:12 AM
I'll post some pics when there is something to see.
The purpose of the experiment is to compare final dry weights and see if the combined weight of the ziploc shrooms is in the neighborhood of what comes off the typical invitro cake. More to come...