Hip's invitro tek [merged]
Posted 08 December 2005 - 04:31 PM
However, any wise mycotopian knows that even if a project takes a turn for the worst, there are valuable lessons to be extracted.
Perhaps the most important aspect of growing invitro with ziplocs is neglect. The test bags that were made up with chunks of PF cakes took off with pins after they were allowed to sit somewhere without being disturbed for at least a couple days. SWIM got a little anxious and decided to peak too much and disturbed some primordia which then stopped maturing.
Another thing to add is perhaps some sort of gas exchange means. Something cheap and readily effective and sterile.... Maybe those little round band-aids could be placed over a hole in the ziploc. It wouldn't take much, just a little help for the cakes anti-trich defenses.
Thai Lipa Yai is not the best invitro strain, but it was all he could spare at the time. Perhaps Texans or B+ next time.
Pins in the ziplocs have been dense little fatties though... hopefully they will put out some good sized ones before contams settle in. I'll drop some pics in another week or so if there's anything interesting to see.
Posted 08 December 2005 - 11:34 PM
Posted 16 January 2006 - 10:57 PM
Posted 16 January 2006 - 11:41 PM
Posted 17 January 2006 - 02:23 AM
Posted 17 January 2006 - 09:19 AM
Posted 17 January 2006 - 09:30 AM
Posted 17 January 2006 - 12:22 PM
do you do this with every strain
you work with...?
Posted 17 January 2006 - 01:11 PM
Posted 18 January 2006 - 02:49 AM
Posted 18 January 2006 - 08:24 AM
This is the last straw; I'm learning agar.
Finding that stuff around here is a bitch...
I suppose that I'll have to break down and order some.