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Panellus Stipticus. Illustrated


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#1 cichlisuite

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Posted 05 February 2009 - 05:29 AM

Hallo, Earthmen!

Not so long ago i got container with Panellus Stipticus mycelium.
I promised to take care and cherish it. I tried to clone it, but unfortunately Pannelus doesn't like :eusa_snoo wheat agar. Other promise was to obtain fruiting.
I have met small difficulties finding necessary substrate with required size of the particles.

So container some time stood without deal and here is that was got there :eek:

Here are some photos. Some of them are double: in light and full darkness taken in one forshortening.

Friends :hugs:, i need some advice how to continue this cycle :eusa_eh: ?
Thanks!

Found related topic: http://mycotopia.net...cent-fungi.html
Some ecological info: http://www.mushroome..._stipticus.html

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Edited by cichlisuite, 05 February 2009 - 05:32 AM.
adjustment

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#2 eatyualive

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Posted 05 February 2009 - 09:58 PM

amazing shots!

#3 cichlisuite

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Posted 06 February 2009 - 05:05 AM

amazing shots!

thanks, but it would be better if i find some apparat with unlimited exposure time. Shots in darkness were taken with following parameters: exp 15 sec, f 2,7, iso800. Unfortuanately, 15 sec is maximum for my compact camera and with iso800 it performs quality below satisfactory :gah: :)


PS
People who use compact Canon cameras, but not satisfied with their possibilities, can increase it using so called CHDK (Canon Hacker’s Development Kit). For example it can learn your compact camera to use 64 sec :headbang: exposure!
for more info see CHDK faq and CHDK wiki

#4 cichlisuite

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Posted 06 February 2009 - 01:10 PM

Heh, boys and girls, here comes 64 seconds exposure with iso100 and iso200. Looks much more nicer :)

Any idea, what organ is it? :eusa_eh:

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#5 MycoViator

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Posted 06 February 2009 - 01:31 PM

These look a lot like marine coral. very very cool stuff.

#6 al-cabar

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Posted 06 February 2009 - 02:01 PM

Krosaf4eG! :eusa_clapcongratulation

#7 cichlisuite

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Posted 06 February 2009 - 02:43 PM

Some morphological details in artistic performance :loveeyes:

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#8 spacecake

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Posted 06 February 2009 - 03:05 PM

Great shots Cichlisuite ! :kewl:

#9 cichlisuite

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Posted 06 February 2009 - 08:13 PM

Here is extremely close up and another file is original size photo (2816x2112pix) special for luminescent fans :)
It taken with iso200 and 64 sec exposure.
In the near future i try to find more capable reflex camera.
upd: perhaps someone interested in Panaeolus papilionaceus/foenisecii prints

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Edited by cichlisuite, 06 February 2009 - 08:21 PM.
upd

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#10 cichlisuite

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Posted 09 February 2009 - 07:56 AM

The substrate of the log is some leafy tree sawdust. It was inoculated in november'08. The Mycelium grew quite slowly.

More magic pictures.
This one with blue flashlight.

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Edited by the_chosen_one, 09 March 2014 - 02:37 PM.


#11 cichlisuite

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Posted 28 February 2009 - 04:33 AM

Here is some update.
I cleared out that this corall is some kind of atypical prefruitbody that forms under conditions of fresh air insufficiency and infraction of light/darkness mode. So I've installed air compressor and put the container on the daylight.
In several days on nearly every offshoot of Panellus corall have formed some kind of premordia.

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Edited by the_chosen_one, 09 March 2014 - 02:38 PM.

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#12 mushhut

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Posted 28 February 2009 - 04:10 PM

Wow amazing pics bro. Are thoes active?

#13 Dr.Hallucination

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Posted 28 February 2009 - 04:45 PM

Beautiful Work:eusa_clap

#14 eatyualive

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Posted 28 February 2009 - 06:11 PM

"archive material"

#15 Myc

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Posted 28 February 2009 - 11:48 PM

Wow amazing pics bro. Are thoes active?


They just glow. Although not poisonous, probably not pleasant to eat based upon reports I've read.

Nice job cichlisuite!
I have a bag of these on the dresser. It's interesting to go to sleep pondering the wonders of these fungi.
I'll have to check out this canon download.
Is it safe? No problems with your camera?

#16 datsun

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Posted 01 March 2009 - 12:38 AM

myc: the last time I looked at the chdk setup it lets you boot onto it, not modifying your cameras bios at all, depending on the camera you have there are TONS of options you can unlock, TIME LAPSE photos like all day, saw a guys bicycle trip miles and miles , wonderful program. I am definetly going to buy a canon camera next time to try this.

[Direct Link]





amazing stuff.

#17 bluethumb

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Posted 01 March 2009 - 01:06 AM

:bow:

#18 Workman

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Posted 01 March 2009 - 02:15 AM

Amazing pictures. And yes, the coral-like formations are from insufficient light and/or lack of fresh air. The same thing happens with CO2 sensitive oyster mushrooms.
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#19 cichlisuite

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Posted 02 March 2009 - 09:24 AM

mushhut, Dr.Hallucination., eatyualive, Myc, bluethumb, Workman, thanks for your comments :)

I'll have to check out this canon download.
Is it safe? No problems with your camera?

as datsun have said CHDK don't modify BIOS of the camera, it's loads from the memory card and exists only in operative memory. so no changes to camera's software doesn't occur. it's 100% safe. the only problem which can appear at entering too short exposure (<1/5000 sec) when this value borders technical possibility of camera's shutter, but it's depends on model. anyway i don't think that such extremly short exposures you ever need :) perhaps only to shot solar protuberances :)

the "pins" continue to grow and here some pics in the dark. as you see bioluminescence is irregular. As it seemed to me it depends not only from arrival of the oxygen, but also from time of the day.

And here is very interesting article "The Role of Superoxide Dismutase in Regulating the Light Emission of Luminescent Fungi" by Osamu Shimomura:

The Role of Superoxide Dismutase in Regulating the Light Emission of Luminescent Fungi
OSAMU SHIMOMURA

Marine Biological Laboratory Woods Hole, MA 02543, USA

Luminescent fungi spontaneously emit light during certain stages of their life cycles. Most of them are luminous during a part of their mycelial stage, but not many of them are luminous when they form fruiting bodies. In the case of Panellus stipticus, both the mycelium and the fruiting body can be luminous, and the emission of light takes place when its luciferin is aerobically oxidized in the presence of the superoxide anion (O2) and a cationic surfactant. It is highly likely that the luminescence reactions of all kinds of luminous fungi are basically the same as that of P. stipticus. In order to determine the factor that makes a fungus luminous or non-luminous, we studied the relations between the light emission of fungi at various growth stages and the contents of luciferin, its precursor, superoxide dismutase (SOD), and catalase, on six species of luminescent fungi: Armillariella mellea, Mycena citricolor, Mycena lux-coeli, Omphlotus olearious, Panellus stipticus, and Pleurotus japonicus. The analysis of the data suggested that the fungi generally contain the components necessary for light emission, but also contain very large amounts of SOD which destroy O2–. If an appreciable amount of SOD is distributed at the site of light emission, the luminescence reaction is prevented. For the reaction to take place, it is essential that the SOD activity at the site is sufficiently low or inhibited, despite the high content of SOD in the whole tissue. Thus, the level of SOD activity at the site of light emission appears to be a limiting factor in regulating the luminescence of fungi.

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Edited by the_chosen_one, 09 March 2014 - 02:39 PM.


#20 Guest_mystic_*

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Posted 02 March 2009 - 12:28 PM

WOWOWWOWOWOWOWOWOOWOWOOWOW very cool. I have came back to this thread at least 10 times. Just neat inspiring to say the least.

Edited by the_chosen_one, 09 March 2014 - 02:39 PM.





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