I was interested to see what the majority of the community did as far as incubating/directly sending to the fruiting chamber freshly cased substrates.
I choose not to. My substrate is colonized and I like to lay that layer and stick it in the fruiting chamber. Its not that it saves anymore time or anything, I just find I get a more even colonization and I don't have to patch or any of that. Overlay is also non-exsistent for me using this method.
So I just wanted to hear some others methods and maybe why they incubate/fruit immediately the fresh cased substrates. Makes for good conversation :)
Do you incubate your freshly cased cubensis substrates?
Started By
Guest_freakachino_*
, Nov 22 2005 03:11 PM
21 replies to this topic
#2
711
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Mycophage
Posted 22 November 2005 - 03:22 PM
FOAF always incubates a lil to allow the myc to grow a lil into the casing layer.
#3
Guest_dial8_*
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Posted 22 November 2005 - 03:42 PM
I voted no. I only incubate if my room temperature is really low for some reason.
#4
Posted 22 November 2005 - 04:56 PM
Had to say yes, although I never put much thought to it. What I do is cover it with foil to choke it for a few days. This stimulates the myc to grow and look for air. That's usually been my primary concern. Now that I think of it, choking works both ways warmer or cooler at room temp with little time difference.
There's some conversation freaky! "TCO chokes his babies!":evil:
There's some conversation freaky! "TCO chokes his babies!":evil:
#5
Omega 11
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Mycophiliac
Posted 22 November 2005 - 05:40 PM
This is interesting. So once you add the casing to the grow chamber, do you allow them light? Or do you wait some period of time before introducing light?
#6
Posted 22 November 2005 - 06:00 PM
After being covered for about 3-5 days I tear off the foil covering and put them in the chamber with light and lots of air. The rhizo should continue to fill the casing layer for a little while and pins will soon follow.This is interesting. So once you add the casing to the grow chamber, do you allow them light? Or do you wait some period of time before introducing light?
#7
Guest_freakachino_*
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Posted 22 November 2005 - 06:21 PM
TCO, I used to do that too, then stopped. I guess I figured most of mine are bulk subs so they've spent time building co2 to induce pinning up until the point I case. When I case is when the fruiting conditions get introduced. Sometimes I even like to take bulk substrates and let them sit open air for a bit, then case, then fruiting chamber.
So now my question is, does the incubation allow for a better pinset because of the "choking" or co2 build up? I've been getting decent pinsets from trying both ways, so I guess I just quit incubating the casing layer. Maybe straight casings and bulk substrate casings would require different casing incubation parameters.
I hope this is making for good conversation :D I like hearing everyones different thoughts and reasons for doing what they do!
So now my question is, does the incubation allow for a better pinset because of the "choking" or co2 build up? I've been getting decent pinsets from trying both ways, so I guess I just quit incubating the casing layer. Maybe straight casings and bulk substrate casings would require different casing incubation parameters.
I hope this is making for good conversation :D I like hearing everyones different thoughts and reasons for doing what they do!
#8
Guest_Peter Cottontail_*
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Posted 22 November 2005 - 06:22 PM
I do as chosen does, except for only 48 hours. The mycleium barely begins to colonize the casing layer so it can absorb moisture, but doesn't have time for overlay.
RR
RR
#9
Guest_freakachino_*
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Posted 22 November 2005 - 06:29 PM
Thats interesting Roger, I'm glad you added to the thread. So like most of the written casing teks say 3-5 days of casing layer incubation, this may be too much. Which can cause for the need of patching, and often bring overlay.
A 48 hour casing incubation sounds like it'd be just right to acheive the co2 build up without having it feed too much on the casing layer.
Also, I have been laying wax paper over them in the fruiting chamber and changing every couple days. Maybe this is enough for a build up to find fresh air?
A 48 hour casing incubation sounds like it'd be just right to acheive the co2 build up without having it feed too much on the casing layer.
Also, I have been laying wax paper over them in the fruiting chamber and changing every couple days. Maybe this is enough for a build up to find fresh air?
#10
Posted 22 November 2005 - 06:43 PM
Ok, I'll admit I peek a little after day 2 or 3! Lol!!! I used to peek at my X-mas presents too!
Just enough choking time to see some of that air starved fuzzy myc start popping up. Too long is not good. Once uncovered it will thicken up. Damn, I've been doing it the same way so long I really don't think about the co2 levels, it's mainly gut feel for me. Something I forgot to add....this is mainly for grain casings...if it makes any difference. The key is what Rodger is saying, the moisture consumption is critical.
Wax paper should be fine freaky, even my saddistic ways give them a little air. It's not a total choking. Just enough to make them scared.:lol:
Just enough choking time to see some of that air starved fuzzy myc start popping up. Too long is not good. Once uncovered it will thicken up. Damn, I've been doing it the same way so long I really don't think about the co2 levels, it's mainly gut feel for me. Something I forgot to add....this is mainly for grain casings...if it makes any difference. The key is what Rodger is saying, the moisture consumption is critical.
Wax paper should be fine freaky, even my saddistic ways give them a little air. It's not a total choking. Just enough to make them scared.:lol:
#11
spacecowboy
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in a major life change-jj
Posted 22 November 2005 - 07:43 PM
I do pretty much the same as TCO too, but cover it loosely whith foil to keep in the mositure instead of starving it of air as it is incubating at 78-82F (same as the grain jar spawn run). I also do it for 3-5 days, I think it helps the grain spawn to recover from the shock of being cased.
#12
golly
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modapotato
Posted 22 November 2005 - 07:57 PM
I think we may have different definitions of the term "incubate".. to me it is returning the cased tray to the same warm conditions used to generate spawn ,for faster growth...
I never purposefully do that, as temp is governed by the room it's in...
Usually i cover w foil for 3 or 4 days mostly to conserve the moisture level of the casing but do not provide any special heated environment...
I never purposefully do that, as temp is governed by the room it's in...
Usually i cover w foil for 3 or 4 days mostly to conserve the moisture level of the casing but do not provide any special heated environment...
#13
tripinstein
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Posted 23 November 2005 - 04:52 AM
I cover with foil and poke just a few holes on top and put back in incubator for like 48 hours. This normally is enough time for the mycelia to recover and to have good signs of growth on the casing layer for me. Sometimes it takes longer but not usually.
I peek too, TCO! I have to be the worlds worst. I can't go a day without looking at all the various projects I have going no matter what stage they are in. Its like an obsession, really. What scientist doesn't monitor his work frequently?
I peek too, TCO! I have to be the worlds worst. I can't go a day without looking at all the various projects I have going no matter what stage they are in. Its like an obsession, really. What scientist doesn't monitor his work frequently?
#14
Posted 23 November 2005 - 11:16 AM
I peek too, TCO!
Glad I'm not the only one Tripi!!!
This is a really cool thread freaky! Stuff I really don't put much thought to it's become so second nature. That's the thing I love about this place, keep ya current and keeps ya thinking!
#15
Omega 11
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Posted 23 November 2005 - 01:58 PM
Yeah, this thread is a nice one. It's answering a lot of questions I've had, thank you TCO, Freakachino, Roger. You all seem very educated on this. One other thing I was wondering is do you usually patch the first few signs of myc in the casing, or leave them to the chamber to continue growth? thanks for all the helpful info.
#16
Guest_freakachino_*
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Posted 23 November 2005 - 02:13 PM
Glad its a good thread for most :)
I just thought it'd be an interesting topic as I never thought much about how I was doing it either and figured some other ideas/ways would be good to read about.
Also, after reading the good comments in this thread I have decided to only incubate my straight grain casings (grains that were not spawned to a bulk substrate). This way the mycelium can recover a bit, then be put to fruit in the chamber. I will go back to doing this to see what advantages it may bring me :)
I will keep not incubating bulk substrate casings though because I find they don't need it. The mycelium doesn't need a recovering process because its already recovered enought to eat the bulk substrate and then its not consuming so much of the casing layer. This just works for me so Im not changing that.
Omega, to answer your question, no I don't patch. I know a lot of folks who do, I just don't. I find if I lay the casing layer good enough it will eat through pretty consistently. To me patching just adds more time etc. I mean, you want it to poke through the casing, if you keep covering it it will just delay pinning even more. The argument to that is a more even pinset because it can come through the casing layer evenly. But, if its multi-spore and not an isolated strain, you don't know how fruiting is going to be anyway. So thats just my opinion of course :)
I just thought it'd be an interesting topic as I never thought much about how I was doing it either and figured some other ideas/ways would be good to read about.
Also, after reading the good comments in this thread I have decided to only incubate my straight grain casings (grains that were not spawned to a bulk substrate). This way the mycelium can recover a bit, then be put to fruit in the chamber. I will go back to doing this to see what advantages it may bring me :)
I will keep not incubating bulk substrate casings though because I find they don't need it. The mycelium doesn't need a recovering process because its already recovered enought to eat the bulk substrate and then its not consuming so much of the casing layer. This just works for me so Im not changing that.
Omega, to answer your question, no I don't patch. I know a lot of folks who do, I just don't. I find if I lay the casing layer good enough it will eat through pretty consistently. To me patching just adds more time etc. I mean, you want it to poke through the casing, if you keep covering it it will just delay pinning even more. The argument to that is a more even pinset because it can come through the casing layer evenly. But, if its multi-spore and not an isolated strain, you don't know how fruiting is going to be anyway. So thats just my opinion of course :)
#18
gema
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XPLORER
Posted 23 November 2005 - 03:34 PM
for cubes I dont bc I get cobweb but for pans I might.
#19
wovotom
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Mycophiliac
Posted 27 November 2005 - 11:29 PM
yes i am currently doing it at the moment
#20
maidenofiron
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Mycotopiate
Posted 30 November 2005 - 12:58 PM
This is really interesting cos i thought from the basic casing tek i read in the archives it says to incubate the casing afterwards, but it never said for how long. so what i did was to "choke" a bunch of my casings and waited untill there was maybe 5-10% fuzzy myc poppin out untill i uncovered them. I then covered up what was pokein out. but heres the thing...i uncovered the casings like 4 days ago and they havent grown at all since they were uncovered. someone mentioned that it wasnt good to "choke" the casings for too long, which i didnt know, and they must have been covered for 5-6 days and i think the reason for that was because i couldnt get the heat up past 70 degrees and so the myc was growin slowly(?). now i can get the heat up to mid 80s but they dont seem to be growing anymore...so am i just being impatient or is there something i should be doing, or did i screw it in the "choking" process? thanks guys!
