dry tissue cloning ?
Posted 16 March 2005 - 12:02 PM
Is it a good idea to dump the peice in h202? I dont get along with h202 very well but I mean this peice of mushroom has been sitting in a jar of dissicant for 6 months with a bunch of coir and vermiculite from the harvest in there with it.
Is a peice the size of a grain of rize about right?
BTW im not using any kind of crazy h202 agar or antibiotic agar. I know that contams will grow too, but was hoping to either cut away the good stuff or do the "hot agar on top" possibly with antibiotic.
I know roger knows about this!
Posted 16 March 2005 - 02:27 PM
Posted 16 March 2005 - 02:53 PM
Posted 16 March 2005 - 06:25 PM
Posted 16 March 2005 - 07:43 PM
maybe drop it in peroxide for a few seconds, let it fizz, then drop that small piece on the agar in front of the flowhood/inside glovebox.
after you get growth cut away any clean myc from contams, and do the same in a new petri.
thats just a guess of what they are going to say to do.
i do remember reading about how you can pour new agar over the contamed agar , and the myc will poke through, you then scrape away the clean myc and put it on a new dish to grow out.
but it really all comes down to someone with first hand experience, and not just a reader like me.
Posted 16 March 2005 - 09:02 PM
Contamination will definitely grow too, but you're learning agar, so jump right in and transfer your healthy mycelium to a new petri dish. Never try to move contamination away from a dish. When contamination shows up, I shut the flow hood off and work in a glovebox. I don't want my flow hood blowing contam spores everywhere. Make a couple of transfers of healthy mycelium away from the original dish, then do the hot agar tek as a final clean. After that, transfer a wedge to rye grain, and away you go. I've used this tek on two year old dry tissue with success.
Posted 16 March 2005 - 09:30 PM
i like the tidbit of info about turning the flowhood off
so when you see contams and myc growing away from it, you move to a glovebox to work . and transfer the seemingly healthy myc from the contamed petri to a new fresh one. how do you prep the glovebox . just bleachwater it down? use an ion generator with it?
this is cool
Posted 17 March 2005 - 01:35 AM
Posted 08 April 2005 - 03:35 PM
Posted 08 April 2005 - 03:38 PM
if it pans out preternaturally.
Posted 08 April 2005 - 07:28 PM
after the tissue is dryed it is my impression that it is hard to revive. from what i've heard even reviving dry tissue on agar normaly results in show growth and little vigor.
Posted 09 April 2005 - 04:55 AM
Posted 09 April 2005 - 10:13 AM
Posted 09 April 2005 - 09:26 PM
RR when re-growing live tissue on agar is it nessacary to hydrate the tissue or do you just stick it on the agar still dry?
Posted 10 April 2005 - 12:31 AM
Posted 10 April 2005 - 07:10 AM
Posted 27 November 2005 - 12:03 PM
tons of Malt extract and potato dextrose and agar powder.
I want to make up some Malt extract agar. would someone be willing to share a winning recipe? Also...i was looking through the archives and found an entry for someone growing dried tissue on agar....how would i do this? I have a piece of cap i saved from some shrooms i had about a year ago...will this grow on agar? will i have to hydrate it....