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Pan Cyan clone experiment


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#21 golly

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Posted 04 December 2005 - 10:17 AM

After 36hours the new clones are progressing nicely -beginning to fluff up.
temp has been around 78f....

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#22 Elf Salvation

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Posted 04 December 2005 - 11:12 AM

:space:
Got somthin good there Golly.

#23 golly

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Posted 05 December 2005 - 10:51 AM

Ok..I'm going to push the envelope a little further and try re-animating a crispy dried panaeolus stem section -I've no idea if it will work but there's a snowstorm comin and i need something to do...:smokin:

#24 Guest_freakachino_*

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Posted 05 December 2005 - 05:25 PM

:lol: Those snowstorms give me lots of time to work too :lol:

Golly, that growth looks nice. Are you worried at all about the moisture holding in the jars while the tissue spreads out?
Are you going to shake it at all?
Do you think this process would work better if the tissue sample was cut into pieces and distributed throughout the jar? Though the pan cyan stems are soooo small that may be hard to accomplish.

Just some questions I thought we could all discuss while we wait :D
Awesome thread!

#25 golly

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Posted 05 December 2005 - 06:32 PM

Thanx...Once the grains in contact with the stem section colo [about 4 days] i will shake em up for a rapid finish ...For a liquid extraction u don't have to wait for complete coverage ...around day 7-9 is good....
I like this method because it's easier to identify contamination on grains than in LC. There is enough air in the sealed jar to complete the process so no filter is needed...
If u wanted to simplify this method even further ,u could just drop the stem sections in full Qts of grain and colo the jars with no need for the LC step. Of course i still need to verify the dependability of the 35%H202 tec
but so far 6 of 6 are kickin...

#26 Guest_freakachino_*

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Posted 05 December 2005 - 07:49 PM

:bow:

So great Golly!!!! I also have some dried tissue from some pans I may try to rehydrate and try something like this with. I think its great you're opening up new simple alternatives to cloning. Its great too you can extract the liquid mycelium without having to let it finish colonization.

I am enjoying this thread a lot!!!!

#27 fahtster

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Posted 06 December 2005 - 04:01 PM

nice golly! :) nice to you see that tek in action. and you were right, it's "faht" hehe. s'all good yo. very curious to see this tek with the pans and the fahtty iso tek others are doing with the pans. :) keep up the great work!

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#28 golly

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Posted 06 December 2005 - 06:11 PM

Thnx faht -you da man..! ..So here's the crispy dried pan cyan stem,rehydrated in a double dunk of water/H2o2 ..then dropped into a qt jar [the twisty thing]
Just in case this doesn't work -there is a regular SA clone in the other side of the jar...we'll see...

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#29 golly

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Posted 06 December 2005 - 06:16 PM

And an update on the other 3 clones started 71 hours ago...they should be ready to shake in another day...

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#30 Guest_freakachino_*

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Posted 06 December 2005 - 11:28 PM

Looking great Golly!!!


Sorry for the mis spelling Fahtster, :o

#31 fahtster

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Posted 07 December 2005 - 01:39 AM

s'all good freak. :) no worries.

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#32 Elf Salvation

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Posted 07 December 2005 - 02:32 AM

Nice, just began somthing similar with airport style lids, one inch wbs in the bottom of three quarts. Multi spore spore, however, I just love the rtv silicone for added sterility. Elephant dung syringe had been in fridge and had some myc growth. I think this will be more fruit full than Lc experiments.

#33 Elf Salvation

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Posted 07 December 2005 - 02:35 AM

Could even grind clone tissue after cleaning and shoot from syringe.:cool:

#34 golly

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Posted 07 December 2005 - 05:17 PM

At day 4 the clones are ready to shake ,for a fast finish...
I'm pretty certain now that this 35%H2o2 clone method is sound..The main drawback however is as hippie stated, that this peroxide is not easy to comeby....On the "gardian of eden" website - it runs 10 bucks a pint +shipping....Sooo I'm going to make up a bunch of clone jars and see what we can substitute thats more commonly available...
I do think that the water rinse bath is essential for allowing immediate growth to begin...
Like a tripple whammy,,, bleach dunk - 3%peroxide dunk - rinse dunk..
Any input is welcome ...
With the growing interest in pan cyan culture and the need to isolate for good fruiting...It would be great to have a simple clone method..

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#35 waylitjim

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Posted 07 December 2005 - 05:35 PM

Great work Golly!
Nice one bringing back Fahts tek,
and applying it to Pan cyans.
:bow:

#36 Guest_freakachino_*

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Posted 07 December 2005 - 06:33 PM

Thats looking great!

I think possibly just a dip in 3% h2o2 with a h2o rinse might be sufficient. Of course only by testing will we know. Thanks for your work with this Golly.

#37 golly

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Posted 07 December 2005 - 07:01 PM

Yeah..there is some logic that would imply that dunking in 35%H2o2 for three seconds, would be the equivelent of dunking in 3%H202 for 36 seconds ...that is on my to do list.....
Referring back to the first post ..all jars injected with "faht's LC" are colonizing well ...I may have to slow em down cause i'll be splittin for the holidayzzz
Plan to spawn New years eve so we can compare the isolate against my usual scattered fruiting results...

#38 HatchetMan

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Posted 07 December 2005 - 07:22 PM

Wouldnt colonisation be quicker if you made those stems into a liquid culture and nocced them up with a syringe?

#39 Guest_freakachino_*

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Posted 07 December 2005 - 07:38 PM

HatchetMan, that is what he's doing, only he's not using LC to start it. He's using the grains to start the tissue growth so he can inject sterile water to withdraw out the cloned tissue mycelium water to inject into his substrate jars.

These are pan cyans too, have you tried to get clean sterile tissue from inside a pan cyan stem? Its not too easy IMHO.

#40 Guest_lost_onabbey_rd_*

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Posted 07 December 2005 - 09:34 PM

the reason he isn't using LC is as he said.. LCs are hard to see contams in..
where as it is much easier to spot contams on a solid substance like grain.
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