Pan Cyan clone experiment
Posted 04 December 2005 - 10:17 AM
temp has been around 78f....
Posted 05 December 2005 - 10:51 AM
Posted 05 December 2005 - 05:25 PM
Golly, that growth looks nice. Are you worried at all about the moisture holding in the jars while the tissue spreads out?
Are you going to shake it at all?
Do you think this process would work better if the tissue sample was cut into pieces and distributed throughout the jar? Though the pan cyan stems are soooo small that may be hard to accomplish.
Just some questions I thought we could all discuss while we wait :D
Posted 05 December 2005 - 06:32 PM
I like this method because it's easier to identify contamination on grains than in LC. There is enough air in the sealed jar to complete the process so no filter is needed...
If u wanted to simplify this method even further ,u could just drop the stem sections in full Qts of grain and colo the jars with no need for the LC step. Of course i still need to verify the dependability of the 35%H202 tec
but so far 6 of 6 are kickin...
Posted 05 December 2005 - 07:49 PM
So great Golly!!!! I also have some dried tissue from some pans I may try to rehydrate and try something like this with. I think its great you're opening up new simple alternatives to cloning. Its great too you can extract the liquid mycelium without having to let it finish colonization.
I am enjoying this thread a lot!!!!
Posted 06 December 2005 - 04:01 PM
Posted 06 December 2005 - 06:11 PM
Just in case this doesn't work -there is a regular SA clone in the other side of the jar...we'll see...
Posted 06 December 2005 - 06:16 PM
Posted 06 December 2005 - 11:28 PM
Sorry for the mis spelling Fahtster, :o
Posted 07 December 2005 - 02:32 AM
Posted 07 December 2005 - 02:35 AM
Posted 07 December 2005 - 05:17 PM
I'm pretty certain now that this 35%H2o2 clone method is sound..The main drawback however is as hippie stated, that this peroxide is not easy to comeby....On the "gardian of eden" website - it runs 10 bucks a pint +shipping....Sooo I'm going to make up a bunch of clone jars and see what we can substitute thats more commonly available...
I do think that the water rinse bath is essential for allowing immediate growth to begin...
Like a tripple whammy,,, bleach dunk - 3%peroxide dunk - rinse dunk..
Any input is welcome ...
With the growing interest in pan cyan culture and the need to isolate for good fruiting...It would be great to have a simple clone method..
Posted 07 December 2005 - 05:35 PM
Nice one bringing back Fahts tek,
and applying it to Pan cyans.
Posted 07 December 2005 - 06:33 PM
I think possibly just a dip in 3% h2o2 with a h2o rinse might be sufficient. Of course only by testing will we know. Thanks for your work with this Golly.
Posted 07 December 2005 - 07:01 PM
Referring back to the first post ..all jars injected with "faht's LC" are colonizing well ...I may have to slow em down cause i'll be splittin for the holidayzzz
Plan to spawn New years eve so we can compare the isolate against my usual scattered fruiting results...
Posted 07 December 2005 - 07:22 PM
Posted 07 December 2005 - 07:38 PM
These are pan cyans too, have you tried to get clean sterile tissue from inside a pan cyan stem? Its not too easy IMHO.
Posted 07 December 2005 - 09:34 PM
where as it is much easier to spot contams on a solid substance like grain.