MAy bee one could run a needle through a stem(for cloning) to extract clean center and have water in syringe to blast tissue into grain
I think over counter 3% H2O2 should work well. I and looking forward to finding out.
Pan Cyan clone experiment
Started By
golly
, Nov 30 2005 06:35 PM
57 replies to this topic
#42
Texas_Bob
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Mycophage
Posted 08 December 2005 - 01:01 PM
Looking Good Golly!! I gotta see how this clone preforms for you.
#43
golly
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modapotato
Posted 08 December 2005 - 05:18 PM
The 3% peroxide has generally been unreliable in past experiments by me and others...The succsess with the stronger H2o2 alows for the easy transfer of large stem pieces to rapidly grow out on the grain for a liquid extraction ,,which can then noc up many quarts...
The whole process should produce fully colonized qts in 18 days...
As for blenderizing the stem tissue - i believe the peroxide would kill [oxidize] the tiny fragments...
You could of course drop the stem tissue directly in to a LC jar after the dunk but i prefer faht's method myself....
The whole process should produce fully colonized qts in 18 days...
As for blenderizing the stem tissue - i believe the peroxide would kill [oxidize] the tiny fragments...
You could of course drop the stem tissue directly in to a LC jar after the dunk but i prefer faht's method myself....
#44
Guest_pcsillypj_*
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Posted 08 December 2005 - 05:31 PM
i hate lc's..i think i'm gonna
do my first cyan clones with this
method..:D
do my first cyan clones with this
method..:D
#45
golly
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Posted 08 December 2005 - 05:33 PM
This is pretty cool - 48hrs and the crispy dried clone sample is now fluffing up ..I'm amazed...The pic's a bit dark but the vertical strand is the stem...:dance:
Attached Thumbnails
#46
Lazlo
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old hand
Posted 08 December 2005 - 08:10 PM
i hate lc's..
Shame on you! Lol! Just kidden....
Golly. If that grain doesn't contaminate, you'll be one bad motha.....:bow:
#47
Guest_xxxsevxxx_*
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Posted 08 December 2005 - 08:11 PM
iodine is the chemical I use when doing clones . its more reliable then peroxide and its a soft chemical that doesnt seem to harm the mushroom tissue what so ever . i use a 25% povidone iodine , I dilute to around 40-60 or 50%-50% with clean bottled water . the reason i dilute is the iodine i have is stronger then avg . the stuff that is in the stores is 10% so you might not need to dilute yours like i do mine .
later VII
later VII
#48
Lazlo
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old hand
Posted 08 December 2005 - 08:12 PM
Ahhhh!!! You're using food grade peroxide for cleansing? Good move.....
#49
Lazlo
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old hand
Posted 08 December 2005 - 08:14 PM
I use iodine (Betadine) as well Seven. Great stuff! Yet steap in price....
#50
golly
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modapotato
Posted 08 December 2005 - 10:26 PM
Seven, can u splain the method u would use with iodine -do u just dunk and drop in the LC jar...? Do u also just use internal tissue...?
#51
Guest_freakachino_*
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Posted 09 December 2005 - 06:36 PM
I too would like to know more of the iodine use. Do you rinse it in sterile water after dunking in the iodine?
#52
Guest_xxxsevxxx_*
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Posted 09 December 2005 - 08:04 PM
i just use iodine swaps that i stole from the hospital after my surgery . u just prepare the mushrooms for surgery so to speak. just like when the doctors cut us open . it doesnt matter if the iodine gets into the cloned material . im not sure if i whould dunk the whole stem or cap into a soltion of iodine . but hey anythings worth trying once or twice . all post more on this soon when i have a mushroom to clone . its simple stuff really .
later VII
later VII
#53
golly
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Posted 10 December 2005 - 09:58 AM
Just started the next round of speriments:....15 clones..
3 in 10% chlorine dip 20 seconds...
3 in iodine dip..[over the counter 2% tincture with 45% alcohol]
3 in 3% peroxide for 45 seconds.
3 in 3% peroxide 20 seconds then chlorine dip 5 seconds.
3 in 71% iso alcohol 15 seconds..
All pieces were followed by there own individual sterile water bath [swirling for 8 seconds and dropped on grain as b4 [1/2 inch crosscut stem sections]
will have pics in 3 and 6 days....
:smokin:
3 in 10% chlorine dip 20 seconds...
3 in iodine dip..[over the counter 2% tincture with 45% alcohol]
3 in 3% peroxide for 45 seconds.
3 in 3% peroxide 20 seconds then chlorine dip 5 seconds.
3 in 71% iso alcohol 15 seconds..
All pieces were followed by there own individual sterile water bath [swirling for 8 seconds and dropped on grain as b4 [1/2 inch crosscut stem sections]
will have pics in 3 and 6 days....
:smokin:
#54
Guest_freakachino_*
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Posted 10 December 2005 - 01:15 PM
Golly, thanks for trying different solutions. Will help us see the possibilities with all the different ones! Great work!
#55
golly
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Posted 10 December 2005 - 01:24 PM
just thought i would try some easily available items to see what happens..
For mysef I'm completey sold on the35%H2o2....
For mysef I'm completey sold on the35%H2o2....
#56
golly
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modapotato
Posted 15 December 2005 - 05:04 PM
Okeedokee...after 51/2 days the results are in....
The worst results go to the Iodine/alc dip [not pictured] with no growth at all and an overly sweet smell....
The alcohol dip showed some very weak growth but also had that sweet bacterial smell...
The bleach and bleach/h2o2 combo showed some growth on all pieces...
The best performance was the 3%h2o2 dip ...niether this or the bleach had any detectable contam smell....
So someone may want to further investigate the 3% solution, as it does appear to be promising -using the water bath rinse...
These samples were also 3rd flush clones which are proly less vigorous than ones taken from the first......
The worst results go to the Iodine/alc dip [not pictured] with no growth at all and an overly sweet smell....
The alcohol dip showed some very weak growth but also had that sweet bacterial smell...
The bleach and bleach/h2o2 combo showed some growth on all pieces...
The best performance was the 3%h2o2 dip ...niether this or the bleach had any detectable contam smell....
So someone may want to further investigate the 3% solution, as it does appear to be promising -using the water bath rinse...
These samples were also 3rd flush clones which are proly less vigorous than ones taken from the first......
Attached Thumbnails
#57
Guest_xxxsevxxx_*
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Posted 16 December 2005 - 07:46 PM
These samples were also 3rd flush clones which are proly less vigorous than ones taken from the first...... third flush clones are as vigorous as first flush just typiclly not as clean . id like to see this expierment copied using agar teks .
later VII
later VII
#58
golly
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Posted 04 January 2006 - 06:21 PM
The progress so far...after having to slow everything down while away fer the holidays -i spawned the clone material to a wormpoo/peat/coir mix and let em sit for 9 days while i was gonzo ...
Birthed 7 days ago from plastic flower pots... so they are like Pan cakes hee...Anyway, pinning density is significantly better with the clones than my past multispore attempts...
I did lose a couple of cakes to mold cause they were in limbo so long... I'm def gonna use this clone method in future [35%H2o2]...Just started some Ausi pans for comparison....
Birthed 7 days ago from plastic flower pots... so they are like Pan cakes hee...Anyway, pinning density is significantly better with the clones than my past multispore attempts...
I did lose a couple of cakes to mold cause they were in limbo so long... I'm def gonna use this clone method in future [35%H2o2]...Just started some Ausi pans for comparison....
