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Pesa pins: invitro saves the day
Started By
Guest_perpetually_*
, Feb 15 2005 12:36 PM
43 replies to this topic
#21
Guest_lost_onabbey_rd_*
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Posted 18 February 2005 - 03:55 PM
ok guys... here is some more candy... pics of last night right before bed... and the pics from this morning.. also a pic of the other pesa casings first little pins :) gonna hopefully get a few good prints. and without further adue...
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#22
sinthetic
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Posted 18 February 2005 - 04:38 PM
wow the amount of growth between last nite and today is spectacular!!
I like the veils staying in one peice when they attached, looks purty
Nice flush!!
I like the veils staying in one peice when they attached, looks purty
Nice flush!!
#23
Guest_dial8_*
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Posted 18 February 2005 - 05:00 PM
Yea, me love the way the veils look in tact too. Just looks so cozy under those caps. :D
#24
mushrush
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Mycophiliac
Posted 18 February 2005 - 05:04 PM
beauty! yum yum......
#25
taoistshredder
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Posted 18 February 2005 - 05:20 PM
Woah - how did I miss this? GORGEOUS!!! It's nice to see the few that love that beautiful open-cap look...I have a bunch of fatass PESAs invitro right now that will sadly not look at lovely...I'll just have to admire yours (until I get my rye spawnbags together hehehe). Be-au-ti-ful!
#26
Guest_lost_onabbey_rd_*
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Posted 18 February 2005 - 11:53 PM
TS... actually this clone came from an pesa invitro jar.. it was the only pesa jar from that run to make it... i'm thinking the print was comtamed b/c out of 4 PF jars and 2 rye jars.. all of them contamed.. i left the contamed pf jars to fruit invitro in hopes of getting something i could clone.. well one of the jars had this fruit growing 3/4 of the way around it.. so it got cleaned and cloned :).. i've very happy as these are some of the most astetically pleasing mushrooms i have ever seen :)
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#27
Guest_thafunkyone_*
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Posted 19 February 2005 - 12:22 AM
Nice pics and nice boomers lost...good stuff...
#28
Guest_lost_onabbey_rd_*
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Posted 19 February 2005 - 02:27 AM
here are the boomers from the casing... that's 106g in my hand.. total weight was 206 without a few caps set aside for printing :).. casing is being dunked and towel dryed thanks guys for all the kind words :) .. now who's ready for round 2!!!
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#29
Lazlo
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Posted 19 February 2005 - 02:34 AM
Those daddies will knock your socks off!!! I love that strain.
#30
nerve
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Mycotopiate
Posted 19 February 2005 - 02:42 AM
Dare ya to eat the handful hehe
Great job.
Great job.
#31
sinthetic
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Posted 19 February 2005 - 03:02 AM
i'd like to see even larger fruits on the next-go-round :)
#32
Guest_lost_onabbey_rd_*
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Posted 19 February 2005 - 03:07 AM
i'm looking forward to the second flush too :).. cake will be rebirthed today before i go to bed.. a nice 4 hour dunk should do it just fine :)
so by the time the other casing is finished flushing it should be pinning up again. :D
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so by the time the other casing is finished flushing it should be pinning up again. :D
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#33
destroy_erase_improve
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Posted 19 February 2005 - 08:31 AM
that handful looks GREAT! i love when they have the lil myc fluff at the bottom and you can press it really slightly and leave cool marks on them. so tell us, how are the stems? hollow, solid? they look really good man. nice growlog
#34
cutty
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Posted 19 February 2005 - 08:43 AM
I dunno how I missed those updated pics, Nice Fruits ya got there....This time I'll pay attention for round 2.
#35
Guest_lost_onabbey_rd_*
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Posted 19 February 2005 - 01:18 PM
stems were pretty thick... i could feel that they started to get a little hallow near the top though.. total wet weight was 206g.. without 4 caps set aside for printing.... that's 106g that i have in my hand.. the second casing is starting to show some little chubby pins so :) .. more pesa porn on the way in a few days :)
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#36
Guest_lost_onabbey_rd_*
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Posted 27 February 2005 - 11:54 PM
well ya... i've had some test the past few weeks so i haven't had time for pics.. but here are a few more... some pics of the second flush from the casing pictured above... weight was 104g wet.. as you can see i cased it after the dunk... i'm hoping the 3rd flush turns out better...
next are some pics of the second casing i was talking about ^^^.. the first flush was 106g wet.. it was dunked and towel dryed... this time no casing was added... trying to figure out which i like better... this is pinning for the second flush... all the pins seem to be on this side one side of the sub..
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next are some pics of the second casing i was talking about ^^^.. the first flush was 106g wet.. it was dunked and towel dryed... this time no casing was added... trying to figure out which i like better... this is pinning for the second flush... all the pins seem to be on this side one side of the sub..
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#37
Hippie3
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Posted 28 February 2005 - 08:10 AM
actually this clone came from an pesa invitro jar.. it was the only pesa jar from that run to make it... i'm thinking the print was comtamed b/c out of 4 PF jars and 2 rye jars.. all of them contamed.. i left the contamed pf jars to fruit invitro in hopes of getting something i could clone.. well one of the jars had this fruit growing 3/4 of the way around it.. so it got cleaned and cloned
utterly amazing.
i've long known that
even partially contam'd jars
often still fruited invitro
but you're the first i've heard
to actually use that fact
to your advantage.
you found a way
without any agar,
to get a good tissue sample
from a bad print.
out-standing.
#38
Guest_lost_onabbey_rd_*
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Posted 28 February 2005 - 03:36 PM
well thank you hipp :)
once i saw the jars going bad i set them in a cooler in the closet for a week or so... i would take them out and expose to light every few days... within a month i saw the first pins appear... all of the jars actually started to pin right next to the contamed areas first... which i though kind of strange...
it bears mentioning that not only did i get a clean tissue sample from that jar.. but by the time i harvested the invitro fruits the myc had taken over the contamed areas... and with a little bleach dunk i got a 2nd flush..
the methiod i used for cloning was just a simple karo cloning tek with a quick h2o2 dunk of the myc material in an clean bathroom... open air on the counter.. i let the fruit dry for a day in open air so it was kinda tough... then wiped the whole outside of the fruit off with alcholo before starting..
i am currently running some dirty PR spores through in hopes of similar results.. i will keep you up dated on how it goes
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once i saw the jars going bad i set them in a cooler in the closet for a week or so... i would take them out and expose to light every few days... within a month i saw the first pins appear... all of the jars actually started to pin right next to the contamed areas first... which i though kind of strange...
it bears mentioning that not only did i get a clean tissue sample from that jar.. but by the time i harvested the invitro fruits the myc had taken over the contamed areas... and with a little bleach dunk i got a 2nd flush..
the methiod i used for cloning was just a simple karo cloning tek with a quick h2o2 dunk of the myc material in an clean bathroom... open air on the counter.. i let the fruit dry for a day in open air so it was kinda tough... then wiped the whole outside of the fruit off with alcholo before starting..
i am currently running some dirty PR spores through in hopes of similar results.. i will keep you up dated on how it goes
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#39
sinthetic
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Posted 28 February 2005 - 03:51 PM
wow sounds like a jar survival story. Nice pics, those are very 'pretty' stems on those mushies.
#40
shedthemonkey
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Posted 01 March 2005 - 12:17 AM
Awesome Lost! I learned a lot from this thread!! YOU THE MAN!!
