Oven sterilization of whole grains log....
Posted 19 December 2005 - 03:15 PM
This is a square of a Golden Oyster clone on malt agar. It's just started to take to the seed today, along with the other 2. 3 jars done in this fassion total. I'll get to the rest when or if they colonize correctly. Lookin good so far...
Posted 19 December 2005 - 04:43 PM
Posted 19 December 2005 - 05:10 PM
Grains were soaked for 36 hours and drained well.
So how do u sterilize in the oven without em drying up laz...? B pretty cool if it works...
I used foil under the filtered lids to hold in the moisture and heat from the sterilizing process. Sterilized for 90 minutes in the oven on 250 degrees. The foil can stay inside the lids. You won't even know it's there, even when taking the lids off. After the MEA squares were placed in to the jars, I put a syringe through the filter to tear the foil for gas exchange. Simple! You can knock up with spores or LC and tear the foil all at once too....
Posted 19 December 2005 - 06:11 PM
Posted 19 December 2005 - 06:46 PM
Posted 19 December 2005 - 07:51 PM
Posted 19 December 2005 - 09:16 PM
Posted 20 December 2005 - 03:04 AM
Posted 20 December 2005 - 09:39 AM
ive had luck with a shortcut on fractional, and its worked 2 outa the 2 times ive did it...
i simmered the cornfor 30 minutes, drained, packed, steamed for 30 minutes, let cool for like 8 hours, and then steamed for 45 minutes, and then let cool overnite, and then nocked up... havent gotten contams
Posted 20 December 2005 - 10:52 PM
Posted 20 December 2005 - 11:11 PM
Posted 21 December 2005 - 01:32 AM
Posted 21 December 2005 - 07:19 AM
Posted 21 December 2005 - 12:26 PM
250 degrees is 250 degrees no matter how you look at it. Yes, steam is more wholesome, but 250 is 250. No mattter what the source of temps is.....
Not to nitpic, but that is incorrect. Pressure adds a whole new dimension to the physics of heat transfer. Moisture also adds a different dimension. That's why it seems so much colder outside when it's a 'wet cold' and why it seems so much hotter in Miami when it's 90F than it does in Arizona when it's 90F.
Posted 21 December 2005 - 12:38 PM
Posted 21 December 2005 - 12:41 PM
Posted 21 December 2005 - 12:54 PM