
Buckaroo's Cloning Tek...{merged}
#21
Posted 07 January 2006 - 01:51 AM
Ah, heck, I'm mixing ideas. I gotta go read some stuff about tempering and oxidation and hardness.
#22
Posted 07 January 2006 - 10:39 AM
#23
Guest_freakachino_*
Posted 08 January 2006 - 07:23 PM
#24
Posted 09 January 2006 - 11:17 AM
Suckerfree - could you elaborate on the electrical/rainbow process? Or just give me a technique or link...
#25
Guest_freakachino_*
Posted 09 January 2006 - 04:50 PM
Do you like half pints or pints for your liquid culture clone jars? I've only used half pints but am thinking pints might work a little better and give more mycelium. Might add a bit more time though.........
#26
Posted 09 January 2006 - 08:02 PM
I started with half pints, but I only use pints now. I fill the jar about 1/2 full so I have plenty of air for growth without needing any kind of venting. How fast your LC will colonize seems to be more a factor of how much tissue you start with and how often you swirl the jar than size. The pint vs. 1/2 pint question seems more a matter of how much LC you want to end up with (his eyes glaze over as he imagines using 1/2 gallon jars for LCs).
10mL of thick clone water swirled 3-5 times a day will colonize a pint in a week or so. Bluehelix has show that with a mechanical stirrer, that time frame can be shortened to 2-3 days.
About 5-6 days in, when the jar is %50-%60 colonized, I shake the living CRAP out of it. This aerates the water and breaks up the mycelium for better "syringe suck up" later on.
Once I have a good container of clone water in distilled water, I use a small amount of it to start LCs and toss the clone original in the refrigerator. The LC starts the next generation and I maintain a "young" master culture to work from.
How do you handle long term culture storage? What's the oldest culture you have successfully worked from?
#27
Posted 15 February 2006 - 02:15 PM
I am a rather simple person with no agar in the cupboards, what would you recommend? Karo? Marbles? Blending? Praying? Cap cuttings? Stem cuttings?
I'm utterly confused :special:
#28
Guest_dial8_*
Posted 15 February 2006 - 02:41 PM
After it has cooled and you have selected the fruit you want to clone you need to do the rest of the work in a glove box. With fruit, karo jar, small dish for hydrogen peroxide, scapel, and tweezers inside the clean glove box you can begin work. With latex gloves on I like to pull the base of the stem in half long ways to reveal the inner tissue. I then take my scapel and cut a small portion of the inner tissue near the base of the stem. Then with tweezers I take the tissue drop it in the dish with hydrogen peroxide for 5 seconds and then transfer to the karo jar (no need to rinse the h2o2 off). I usually make at least 3 jars just to be on the safe side.
#29
Posted 15 February 2006 - 02:45 PM
Thanks so much for your help.

#30
Guest_dial8_*
Posted 15 February 2006 - 02:55 PM
No problem, man.
Here is a link that may help. [broken link]
I do not cut and peel like nan describes in this link. Like I said, I pull the stem open along the mushroom's long axis to reveal the inner tissue. Not only is this approach cleaner, imo, but it also leaves the inner tissue frazzled so more mycelium will come into contact with the karo water.
Edited by Sidestreet, 02 October 2016 - 03:44 PM.
removed broken link
#31
Posted 15 February 2006 - 03:07 PM
can you then take some of this solution after its' incubation period (how many days do you let it sit?) and inject a syringe-full into another Karo jar or do you always start with a fresh piece of innards for each jar of solution?
Again, thank you-thank you:hippie:
#32
Guest_dial8_*
Posted 15 February 2006 - 03:16 PM
I usually let mine incubate for 14 to 20 days. If at that time I am not ready t use it i stick it in the fridge. They can stay good in the fridge for quite a while.
#33
Posted 15 February 2006 - 03:42 PM
#34
Guest_dial8_*
Posted 15 February 2006 - 03:50 PM
#35
Posted 15 February 2006 - 04:02 PM
#36
Posted 15 February 2006 - 09:36 PM
#37
Posted 16 February 2006 - 01:21 AM
Inner tissue blended in a hydrogen peroxide / water solution -----> innoculation of sterilized jars -------> easiest way to clone that I've found.
#38
Posted 16 February 2006 - 03:37 AM
I have 26 casings in fruiting chambers at the moment and so came the question about cloning for my next project...I'm wanting to try the Straw Log.
I've always multi-spored it and so it's time to do something different. I can't wait to see which strain comes up first and how aggressively.
I may try both the Karo and Cardboard teks for comparison. Either one seems easy enough!
I appreciate all your inputs. It's great to come home from work and have your hobby questions answered:hippie:
#39
Posted 18 February 2006 - 09:35 AM
i've heard of cultures 2 years old being revived
but they grew out with diminished vigor.
archive material
#40
Posted 18 February 2006 - 01:56 PM
long-term storage?
I was thinking with a little liquid or substrate,
allowed to dry in a jar with a poly or tyvek filter,
that it might last indefinitely.
Then it could be rehydrated and used to
innoculate. Would drying the culture damage
its future potential?