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Texan Isolates


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#1 fucgubarn

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Posted 01 January 2006 - 05:41 AM

Happy New Year and best of luck for everyone!

1.spores on PDA
2.several transfers to clean it up
3.after cleaning waited for the new petridishes to fully colonize
4.after full colonization of the petris waited a little more for the pin heads to appear
5.when the pin heads grew up, take and put the biggest and coolest ones on new PDA
6.grew some rhizo mycelium
7.made liquid
8.innoculated oat grains in jars
9.pour the colonized oats in the plastic box
10.let recover for 3-4 days
11.cased with a thin layer of vermiculite only

so besicaly its a clone but straight from the petridish

hope you enjoy the pix
thanks :):)

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#2 siam_jim

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Posted 01 January 2006 - 07:07 AM

damn!:cool:
peace happy new year fbarn and all
siam

#3 shedthemonkey

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Posted 01 January 2006 - 10:07 AM

Very Nice! Congrats and thanks for the pix!

#4 waylitjim

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Posted 01 January 2006 - 08:43 PM

Great work as always Fucgubarn! Enjoy the fruits of your labor. ;)

#5 SharkieJones

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Posted 01 January 2006 - 09:20 PM

Very nice as usual.

#6 Elf Salvation

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Posted 02 January 2006 - 01:44 AM

:amazed:

Lookin real good

#7 fucgubarn

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Posted 02 January 2006 - 02:02 AM

thanks :):)
forgot to say thank you to the person who sent me the spores,
thank you!
here is his picture and where it all started from, a wild cubie from Texas
:)

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#8 Lazlo

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Posted 02 January 2006 - 12:20 PM

Man, that specimen looks fat outdoors huh? Very nice as usual Barn...

#9 Guest_dial8_*

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Posted 03 January 2006 - 09:41 AM

Damn fine job, barn. So you took the actual mushroom that grew off the agar and cloned it, right?

#10 fucgubarn

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Posted 03 January 2006 - 03:01 PM

"you took the actual mushroom that grew off the agar and cloned it, right?"

yes,but it was kind of longer than that and not accurately said above,
have saved the pictures from the lost thread and will retell the story in short,

1,2
is the print as it came to me,the right low angle was cut and spores taken from there

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#11 fucgubarn

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Posted 03 January 2006 - 03:04 PM

3,4,5,6,7
is the first transfer,spores to PDA,mold and bacteria also growing :)

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#12 fucgubarn

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Posted 03 January 2006 - 03:07 PM

,9,10,11,12
the second transfer,looking more clean now :)

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#13 fucgubarn

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Posted 03 January 2006 - 03:10 PM

13,14,15,16,17.
third transfer,everything is clean now :)

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#14 fucgubarn

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Posted 03 January 2006 - 03:15 PM

18,19,20,21,22
from petri to liquid :)

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#15 fucgubarn

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Posted 03 January 2006 - 03:16 PM

24,25
from liquid to cakes and colonizing quite well :)

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#16 fucgubarn

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Posted 03 January 2006 - 03:20 PM

26,27,28,29,30
cakes beginning to fruit but i just was not there for them and they all died out of thirst,it was the summer time,
all looked promising,unfortunately i fucked up

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#17 fucgubarn

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Posted 03 January 2006 - 03:24 PM

31,32,33,34,35
ok,back to second transfer,
pins and the inhibition line between substrains

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#18 fucgubarn

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Posted 03 January 2006 - 03:27 PM

36,37,38,39,40,41
the mushrooms from the previous post on PDA

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#19 Guest_dial8_*

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Posted 03 January 2006 - 03:30 PM

Thanks, barn. Well written and documented.

#20 fucgubarn

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Posted 03 January 2006 - 03:30 PM

44,45,46
mycelium grown from the fruitbodyes to liquid,
here you can see one of the petries broken,got a phone call during the working with this,
grandmama was not well and had to just blow everything and run,finaly she was well and the petri not contamined :),cool eh

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