39 replies to this topic

[fucgubarn]

Happy New Year and best of luck for everyone!

1.spores on PDA
2.several transfers to clean it up
3.after cleaning waited for the new petridishes to fully colonize
4.after full colonization of the petris waited a little more for the pin heads to appear
5.when the pin heads grew up, take and put the biggest and coolest ones on new PDA
6.grew some rhizo mycelium
7.made liquid
8.innoculated oat grains in jars
9.pour the colonized oats in the plastic box
10.let recover for 3-4 days
11.cased with a thin layer of vermiculite only

so besicaly its a clone but straight from the petridish

hope you enjoy the pix
thanks :):)

Attached Thumbnails

• 
• 
• 
• 
• 
• 
• 
• 
• 
• 
• 
• 

[siam_jim]

damn!
peace happy new year fbarn and all
siam

[shedthemonkey]

Very Nice! Congrats and thanks for the pix!

[waylitjim]

Great work as always Fucgubarn! Enjoy the fruits of your labor. ;)

[SharkieJones]

Very nice as usual.

[Elf Salvation]

:amazed:

Lookin real good

[fucgubarn]

thanks :):)
forgot to say thank you to the person who sent me the spores,
thank you!
here is his picture and where it all started from, a wild cubie from Texas
:)

Attached Thumbnails

• 

[Lazlo]

Man, that specimen looks fat outdoors huh? Very nice as usual Barn...

[fucgubarn]

"you took the actual mushroom that grew off the agar and cloned it, right?"

yes,but it was kind of longer than that and not accurately said above,
have saved the pictures from the lost thread and will retell the story in short,

1,2
is the print as it came to me,the right low angle was cut and spores taken from there

Attached Thumbnails

• 
• 

[fucgubarn]

3,4,5,6,7
is the first transfer,spores to PDA,mold and bacteria also growing :)

Attached Thumbnails

• 
• 
• 
• 
• 

[fucgubarn]

,9,10,11,12
the second transfer,looking more clean now :)

Attached Thumbnails

• 
• 
• 
• 

[fucgubarn]

13,14,15,16,17.
third transfer,everything is clean now :)

Attached Thumbnails

• 
• 
• 
• 
• 

[fucgubarn]

18,19,20,21,22
from petri to liquid :)

Attached Thumbnails

• 
• 
• 
• 
• 

[fucgubarn]

24,25
from liquid to cakes and colonizing quite well :)

Attached Thumbnails

• 
• 

[fucgubarn]

26,27,28,29,30
cakes beginning to fruit but i just was not there for them and they all died out of thirst,it was the summer time,
all looked promising,unfortunately i fucked up

Attached Thumbnails

• 
• 
• 
• 
• 

[fucgubarn]

31,32,33,34,35
ok,back to second transfer,
pins and the inhibition line between substrains

Attached Thumbnails

• 
• 
• 
• 
• 

[fucgubarn]

36,37,38,39,40,41
the mushrooms from the previous post on PDA

Attached Thumbnails

• 
• 
• 
• 
• 
• 

[fucgubarn]

44,45,46
mycelium grown from the fruitbodyes to liquid,
here you can see one of the petries broken,got a phone call during the working with this,
grandmama was not well and had to just blow everything and run,finaly she was well and the petri not contamined :),cool eh

Attached Thumbnails

• 
• 
•